Effect of nitrogen supply rate on disease resistance in tomato depends on the pathogen

The aim of this study was to investigate the effect of tissue nitrogen concentration, as a consequence of nitrogen supply rate, on the susceptibility of tomato plants to three pathogens. We varied tissue N concentration by supplying N at different rates by adding nitrate in different, exponentially increasing amounts to the nutrient solution on which the tomato plants were grown. Separate experiments were carried out to test susceptibility of tomato plants to the bacterial speck-causing Pseudomonas syringae pv tomato, to the wilt agent Fusarium oxysporum f.sp. lycopersici and to tomato powdery mildew caused by Oidium lycopersicum. The effect of tissue N concentration appeared to be highly pathogen-dependent: there was no effect on susceptibility to F. oxysporum, but susceptibility to P. syringae and O. lycopersicum increased significantly with increasing N concentration. We have previously demonstrated the opposite for susceptibility to Botrytis cinerea: decreasing susceptibility with increasing N concentration. The apparent contradictory effects are discussed in relation to the effect of N supply on both the nutritional value of the plant tissue to the pathogen and on the concentration of resistance-related compounds. We conclude that the effect of changing both characteristics on disease susceptibility is highly pathogen-specific and is probably dependent on differences in resource requirements of the pathogen or the sensitivity of the pathogen to plant resistance reactions or on both these factors.

[1]  A. Collmer,et al.  The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and tomato are encoded by an operon containing Yersinia ysc homologs and elicit the hypersensitive response in tomato but not soybean. , 1995, Molecular plant-microbe interactions : MPMI.

[2]  H. Komada,et al.  Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. , 1975 .

[3]  Nancy E. Stamp,et al.  DIFFERENTIAL RESPONSES OF GROWTH AND TWO SOLUBLE PHENOLICS OF TOMATO TO RESOURCE AVAILABILITY , 1996 .

[4]  A. Engelhard,et al.  Soilborne Plant Pathogens: Management of Diseases with Macro- and Microelements , 1990 .

[5]  A. Taylor,et al.  Spore Yield of Pathogens in Investigations of the Race-Specificity of Host Resistance , 1976 .

[6]  M. Noordeloos,et al.  Studies in plant pathogenic fungi—II. On some powdery mildews (Erysiphales) recently recorded from the Netherlands , 1989 .

[7]  R. Dixon,et al.  Stress-Induced Phenylpropanoid Metabolism. , 1995, The Plant cell.

[8]  P. Lindhout,et al.  Screening wild Lycopersicon species for resistance to powdery mildew (Oidium lycoperiscum) , 2004, Euphytica.

[9]  R. Niks,et al.  The resistance to powdery mildew (Oidium lycopersicum) in Lycopersicon species is mainly associated with hypersensitive response , 1998, European Journal of Plant Pathology.

[10]  Gert B. Eijkel,et al.  Relative growth rate correlates negatively with pathogen resistance in radish: the role of plant chemistry , 1996 .

[11]  T. Ingestad,et al.  Nitrogen Stress in Birch Seedlings. I. Growth Technique and Growth , 1979 .

[12]  M. Jeger,et al.  Nitrogen availability and susceptibility of tomato leaves to Botrytis cinerea , 1999, Plant and Soil.

[13]  R. Hutson,et al.  Phytoalexins and tyloses in tomato cultivars infected with Fusarium oxysporum f.sp. lycopersici or Verticillium albo-atrum , 1980 .

[14]  F. Woodward,et al.  The effects of host carbon dioxide, nitrogen and water supply on the infection of wheat by powdery mildew and aphids , 1993 .

[15]  R. Cook,et al.  Tomato powdery mildew. , 1988 .

[16]  R. Whitbread,et al.  Effect of elevated concentrations of CO2on infection of barley byErysiphe graminis , 1996 .

[17]  B. Jensen,et al.  Nitrogen-induced changes in colony density and spore production of Erysiphe graminis f.sp. hordei on seedlings of six spring barley cultivars , 1997 .

[18]  D. Huber,et al.  Nitrogen form and plant disease. , 1974, Annual review of phytopathology.

[19]  M. Madkour,et al.  Variation in sensitivity to tomatine and rishitin among isolates ofFusarium oxysporumf.sp.lycopersici, and strains not pathogenic on tomato , 1996 .

[20]  C. H. Beckman,et al.  The Nature of Wilt Diseases of Plants , 1987 .

[21]  A. Boudet,et al.  Enhanced resistance of tomato plants to Fusarium by controlled stimulation of their natural phenolic production , 1978 .

[22]  P. Spencer-Phillips.  Function of Fungal Haustoria in Epiphytic and Endophytic Infections , 1997 .

[23]  A. Engelhard,et al.  Management of fusarium wilt of vegetables and ornamentals by macro- and microelement nutrition , 1989 .

[24]  K. Murakami,et al.  Relationship between Mineral Nutrition and Plant Disease , 2004 .

[25]  D. Herms,et al.  The Dilemma of Plants: To Grow or Defend , 1992, The Quarterly Review of Biology.

[26]  F. Stuart Chapin,et al.  Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory , 1983 .

[27]  E. Hoffland,et al.  Nitrogen Availability and Defense of Tomato Against Two-spotted Spider Mite , 2000, Journal of Chemical Ecology.

[28]  S. Blankenship,et al.  Drip fertigation with nitrogen and potassium and postharvest susceptibility to bacterial soft rot of bell peppers , 1994 .

[29]  D. Cuppels,et al.  Disease Development and Changes in the Natural Pseudomonas syringae pv. tomato Populations on Field Tomato Plants. , 1999, Plant disease.

[30]  B. Danieli,et al.  3-Hydroxyacetophenone in carnations is a phytoanticipin active against Fusarium oxysporum f. sp. dianthi , 1996 .

[31]  N. Matsuyama.  Effect of Nitrogenous Fertilizer on Biochemical Processes that Could Affect Lesion Size of Rice Blast , 1973 .

[32]  J. Whipps,et al.  Characteristics and host range of tomato powdery mildew , 1998 .