Candidate genes and QTLs for fruit ripening and softening in melon

Different factors affect the quality of melon fruit and among them long shelf life is critical from the consumer’s point of view. In melon, cultivars showing both climacteric and non-climacteric ripening types are found. In this study we have investigated climacteric ripening and fruit softening using a collection of near-isogenic lines (NILs) derived from the non-climacteric melon parental lines PI 161375 (SC) and “Piel de Sapo” (PS). Surprisingly, we found that QTL eth3.5 in NIL SC3-5b induced a climacteric-ripening phenotype with increased respiration and ethylene levels. Data suggest that the non-climacteric phenotypes from PI 161375 and “Piel de Sapo” may be the result of mutations in different genes. Several QTLs for fruit flesh firmness were also detected. Candidate genes putatively involved in ethylene regulation, biosynthesis and perception and cell wall degradation were mapped and some colocations with QTLs were observed. These results may provide additional data towards understanding of non-climacteric ripening in melon.

[1]  C. Gessler,et al.  Mapping quantitative physiological traits in apple (Malus × domestica Borkh.) , 2003, Plant Molecular Biology.

[2]  J. Pech,et al.  Ripening-associated biochemical traits of Cantaloupe Charentais melons expressing an antisense ACC oxidase transgene , 1997 .

[3]  P. Arús,et al.  Identification of quantitative trait loci involved in fruit quality traits in melon (Cucumis melo L.) , 2004, Theoretical and Applied Genetics.

[4]  Oda,et al.  Cloning of an auxin-responsive 1-aminocyclopropane-1-carboxylate synthase gene (CMe-ACS2) from melon and the expression of ACS genes in etiolated melon seedlings and melon fruits. , 2000, Plant science : an international journal of experimental plant biology.

[5]  S Rozen,et al.  Primer3 on the WWW for general users and for biologist programmers. , 2000, Methods in molecular biology.

[6]  P. Arús,et al.  Single-nucleotide polymorphisms detected in expressed sequence tags of melon (Cucumis melo L.). , 2004, Genome.

[7]  J. Doyle,et al.  Isolation of plant DNA from fresh tissue , 1990 .

[8]  P. Arús,et al.  Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375 , 2005, Theoretical and Applied Genetics.

[9]  Mondher Bouzayen,et al.  Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon. , 2007, Journal of experimental botany.

[10]  D. Brummell,et al.  Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants , 2001, Plant Molecular Biology.

[11]  J. Giovannoni,et al.  Fruit ripening mutants yield insights into ripening control. , 2007, Current opinion in plant biology.

[12]  J. Pech,et al.  Molecular and Genetic Characterization of a Non-Climacteric Phenotype in Melon Reveals Two Loci Conferring Altered Ethylene Response in Fruit1 , 2002, Plant Physiology.

[13]  Raffaele Testolin,et al.  Mapping With a Few Plants: Using Selective Mapping for Microsatellite Saturation of the Prunus Reference Map , 2005, Genetics.

[14]  H. V. van Leeuwen,et al.  Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana , 2003, Plant Molecular Biology.

[15]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[16]  C. Kuhlemeier,et al.  Growth and development. The diversity of plant development. , 2007, Current opinion in plant biology.

[17]  C. Dunnett.  A Multiple Comparison Procedure for Comparing Several Treatments with a Control , 1955 .

[18]  Paxton Payton,et al.  Use of genomics tools to isolate key ripening genes and analyse fruit maturation in tomato. , 2002, Journal of experimental botany.

[19]  Graham J King,et al.  A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening , 2006, Nature Genetics.

[20]  R. E. Pitt,et al.  RHEOLOGY OF APPLE AND POTATO TISSUE AS AFFECTED BY CELL TURGOR PRESSURE , 1986 .

[21]  M. Sonoda,et al.  Isolation and characterization of three DREB/ERF-type transcription factors from melon (Cucumis melo) , 2006 .

[22]  P. Arús,et al.  MELOGEN: an EST database for melon functional genomics , 2007, BMC Genomics.

[23]  A. Monfort,et al.  Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.) , 2005, Theoretical and Applied Genetics.

[24]  P. Arús,et al.  Construction of a reference linkage map for melon. , 2001, Genome.

[25]  Adel A. Kader,et al.  Post Harvest Technology of Horticultural Crops , 1991 .

[26]  T. Miki,et al.  Nucleotide Sequence of a cDNA for 1-Aminocyclopropane-1-Carboxylate Synthase from Melon Fruits , 1995, Plant physiology.

[27]  M. Causse,et al.  Genetic analysis of organoleptic quality in fresh market tomato. 1. Mapping QTLs for physical and chemical traits , 2001, Theoretical and Applied Genetics.

[28]  K. Higashi,et al.  Stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon. , 1999, Plant physiology.

[29]  M. Causse,et al.  QTL analysis of fruit quality in fresh market tomato: a few chromosome regions control the variation of sensory and instrumental traits. , 2002, Journal of experimental botany.

[30]  J. Vrebalov,et al.  A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus , 2002, Science.

[31]  R. Berka,et al.  Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly. , 1998, Plant physiology.

[32]  J. Pech,et al.  Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits , 1996, Nature Biotechnology.

[33]  P. Arús,et al.  Identification of Melon Fruit Quality Quantitative Trait Loci Using Near-isogenic Lines , 2008 .

[34]  D. Zamir,et al.  An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. , 1995, Genetics.

[35]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[36]  J. Ecker,et al.  Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. , 1998, Genes & development.

[37]  M. Devaux,et al.  Physiological relationships among physical, sensory, and morphological attributes of texture in tomato fruits. , 2007, Journal of experimental botany.

[38]  Cornelius S. Barry,et al.  Ripening in the tomato Green-ripe mutant is inhibited by ectopic expression of a protein that disrupts ethylene signaling , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Mikihiro Yamamoto,et al.  Expression of ACC Synthase and ACC Oxidase Genes in Melons Harvested at Different Stages of Maturity , 1999 .

[40]  J. Labavitch,et al.  The linkage between cell wall metabolism and fruit softening: looking to the future , 2007 .

[41]  M. Vicente,et al.  Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon , 2000, Theoretical and Applied Genetics.

[42]  J. Pech,et al.  Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.) , 1996, Molecular and General Genetics MGG.

[43]  J. Labavitch,et al.  Cell turgor changes associated with ripening in tomato pericarp tissue. , 1991, Plant physiology.

[44]  W. Haddon,et al.  Biochemical Diversity among the 1-Amino-cyclopropane-1-Carboxylate Synthase Isozymes Encoded by the Arabidopsis Gene Family* , 2003, Journal of Biological Chemistry.

[45]  P. Arús,et al.  Estimating the Genetic Architecture of Fruit Quality Traits in Melon Using a Genomic Library of Near Isogenic Lines , 2007 .

[46]  Hideyuki Takahashi,et al.  The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers. , 2000, Plant & cell physiology.