Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) on healing in 3-wall intrabony defects in dogs.

BACKGROUND Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier is being evaluated as a candidate therapy for periodontal regeneration. The objective of this study was to evaluate regeneration of alveolar bone and cementum, and associated root resorption and ankylosis following surgical implantation of rhBMP-2/ACS in a canine clinical model. METHODS Bilateral 3-wall intrabony periodontal defects were surgically induced in the premolar region in the maxilla and mandible in 8 young adult Korean mongrel dogs. The defects in each animal received rhBMP-2/ACS (rhBMP-2 at 0.2 mg/ml, total implant volume/defect approximately 0.1 ml) or buffer/ACS, or served as sham-operated controls. Surgeries were sequenced for each animal to provide postmortem observations following 8- and 24-week healing intervals. Treatment outcomes were evaluated using clinical, radiographic, and histometric parameters. RESULTS Surgical implantation of rhBMP-2/ACS resulted in accelerated enhanced bone formation in the 3-wall intrabony periodontal defects but in no apparent enhancement of cementum regeneration. rhBMP-2/ACS did not appear to be associated with aberrant healing events such as root resorption and ankylosis under these simulated clinical conditions. CONCLUSIONS Surgical implantation of rhBMP-2/ACS may be used safely to support regeneration of alveolar bone in intrabony periodontal defects in dogs without aberrant events such as root resorption or ankylosis complicating the regenerative procedure. rhBMP-2/ACS does not appear to have a significant effect on cementum regeneration and formation of a functional periodontal ligament in this model.

[1]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of transforming growth factor-beta 1 on alveolar bone and cementum regeneration. , 2000, Journal of clinical periodontology.

[2]  F. Hughes,et al.  Effects of occlusal loading on ankylosis, bone, and cementum formation during bone morphogenetic protein-2-stimulated periodontal regeneration in vivo. , 1999, Journal of periodontology.

[3]  L. Trombelli,et al.  Periodontal repair in dogs: histologic observations of guided tissue regeneration with a prostaglandin E1 analog/methacrylate composite. , 1999, Journal of clinical periodontology.

[4]  J. Wozney,et al.  Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment. , 1999, Journal of clinical periodontology.

[5]  K. Selvig,et al.  Periodontal wound healing and regeneration. , 1999, Periodontology 2000.

[6]  U. Wikesjö,et al.  Effect of a calcium sulfate implant with calcium sulfate barrier on periodontal healing in 3-wall intrabony defects in dogs. , 1998, Journal of periodontology.

[7]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of recombinant human transforming growth factor-beta1 on guided tissue regeneration. , 1998, Journal of clinical periodontology.

[8]  F. Hughes,et al.  The effect of root surface demineralization on bone morphogenetic protein-2-induced healing of rat periodontal fenestration defects. , 1998, Journal of periodontology.

[9]  F. Hughes,et al.  Effect of two delivery systems for recombinant human bone morphogenetic protein-2 on periodontal regeneration in vivo. , 1998, Journal of periodontal research.

[10]  W. Giannobile,et al.  Recombinant human osteogenic protein-1 (OP-1) stimulates periodontal wound healing in class III furcation defects. , 1998, Journal of periodontology.

[11]  J. Wozney,et al.  Recombinant Human Bone Morphogenetic Protein-2 Promotes Wound Healing in Rat Periodontal Fenestration Defects , 1997, Journal of dental research.

[12]  I. Ishikawa,et al.  Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs. , 1997, Journal of periodontology.

[13]  J. Wozney,et al.  Periodontal repair in dogs: evaluation of rhBMP-2 carriers. , 1996, The International journal of periodontics & restorative dentistry.

[14]  U. Wikesjö,et al.  Periodontal regenerative potential of space-providing expanded polytetrafluoroethylene membranes and recombinant human bone morphogenetic proteins. , 1995, Journal of periodontology.

[15]  J. Wozney,et al.  Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration. , 1995, Journal of periodontology.

[16]  U. Wikesjö,et al.  Periodontal repair in dogs: space provision by reinforced ePTFE membranes enhances bone and cementum regeneration in large supraalveolar defects. , 1994, Journal of periodontology.

[17]  P. McMillan,et al.  Periodontal repair in dogs: expanded polytetrafluoroethylene barrier membranes support wound stabilization and enhance bone regeneration. , 1993, Journal of periodontology.

[18]  K. Selvig,et al.  Significance of early healing events on periodontal repair: a review. , 1992, Journal of periodontology.

[19]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of a composite graft protocol on healing in supraalveolar periodontal defects. , 1992, Journal of periodontology.

[20]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of root surface treatment with stannous fluoride or citric acid on root resorption. , 1991, Journal of periodontology.

[21]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of wound stabilization on healing. , 1990, Journal of periodontology.

[22]  U. Wikesjö,et al.  Periodontal repair in dogs: effect of allogenic freeze-dried demineralized bone matrix implants on alveolar bone and cementum regeneration. , 1998, Journal of periodontology.

[23]  M. Heliotis,et al.  Induction of cementogenesis by recombinant human osteogenic protein-1 (hop-1/bmp-7) in the baboon (Papio ursinus). , 1996, Archives of oral biology.

[24]  I. Ishikawa Regenerative therapy in periodontal diseases. Histological observations after implantation of rhBMP-2 in the surgically created periodontal defects in adult dogs , 1994 .

[25]  U. Wikesjö,et al.  Periodontal repair in dogs. Healing patterns in large circumferential periodontal defects. , 1991, Journal of clinical periodontology.

[26]  U. Wikesjö,et al.  Periodontal repair in dogs. Effect of heparin treatment of the root surface. , 1991, Journal of clinical periodontology.

[27]  R. Genco,et al.  Repair of periodontal furcation defects in beagle dogs following reconstructive surgery including root surface demineralization with tetracycline hydrochloride and topical fibronectin application. , 1988, Journal of clinical periodontology.