Capillary electrophoretic immunoassays.

Capillary electrophoretic immunoassay (CEIA) has recently emerged as a new analytical technique. CEIA, when combined with sensitive detection methods such as laser induced fluorescence (LIF), offers several advantages over conventional immunoassays. CEIA can perform rapid separations with high mass sensitivity, simultaneously determine multiple analytes and is compatible with automation. The objective of this review is to describe the applications of CE in antibody related studies, focussing especially on recent developments of CEIA technique. The principles for competitive and non-competitive CEIA are described with examples. Several detection methods and various applications are summarized and future developments in CEIA are speculated. CEIA has many potential applications, especially if the throughput is improved by using either multicapillary array or microchips with multiple channels.

[1]  Robert T. Kennedy,et al.  Rapid Immunoassays Using Capillary Electrophoresis with Fluorescence Detection , 1993 .

[2]  Edward S. Yeung,et al.  Differences in the chemical reactivity of individual molecules of an enzyme , 1995, Nature.

[3]  Fu-Tai A. Chen.  Characterization of charge-modified and fluorescein-labeled antibody by capillary electrophoresis using laser-induced fluorescence Application to immunoassay of low level immunoglobulin A , 1994 .

[4]  L. Martin.  Antibody fragment separations by capillary zone electrophoresis. , 1996, Journal of chromatography. B, Biomedical applications.

[5]  T. Pritchett,et al.  Capillary Electrophoresis–Based Immunoassays , 1995, Bio/Technology.

[6]  Kazuyuki Nakamura,et al.  Complete separation of anti‐hapten antibodies by two‐dimensional affinity electrophoresis , 1989, Electrophoresis.

[7]  F. Regnier,et al.  Liquid chromatography based enzyme-amplified immunological assays in fused-silica capillaries at the zeptomole level. , 1993, Analytical chemistry.

[8]  B. Karger,et al.  Affinity probe capillary electrophoresis: analysis of recombinant human growth hormone with a fluorescent labeled antibody fragment. , 1994, Analytical chemistry.

[9]  N. Danielson,et al.  Determination of minute enzymatic activities by means of capillary electrophoretic techniques. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[10]  E. Yeung,et al.  Variability of intracellular lactate dehydrogenase isoenzymes in single human erythrocytes. , 1994, Analytical chemistry.

[11]  Thomas M. Li,et al.  High-performance capillary electrophoresis as a fast inprocess control method for enzyme-labelled monoclonal antibody conjugates , 1991 .

[12]  G. Whitesides,et al.  Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis. , 1995, Analytical chemistry.

[13]  R. G. Nielsen,et al.  Separation of antibody-antigen complexes by capillary zone electrophoresis, isoelectric focusing and high-performance size-exclusion chromatography. , 1991, Journal of chromatography.

[14]  F. Regnier,et al.  Electrophoretically mediated microanalysis of leucine aminopeptidase in complex matrices using time-resolved laser-induced fluorescence detection. , 1993, Analytical chemistry.

[15]  E. Yeung,et al.  Laser-based particle-counting microimmunoassay for the analysis of single human erythrocytes. , 1994, Analytical chemistry.

[16]  Fu-Tai A. Chen,et al.  Characterization of proteins by capillary electrophoresis in fused‐silica columns: Review on serum protein analysis and application to immunoassays , 1994, Electrophoresis.

[17]  F T Chen,et al.  Analysis of structural specificity in antibody-antigen reactions by capillary electrophoresis with laser-induced fluorescence detection. , 1994, Journal of chromatography. A.

[18]  F. Regnier,et al.  Synthesis and evaluation of epoxy polymer coatings for the analysis of proteins by capillary zone electrophoresis , 1992 .

[19]  R. Kennedy,et al.  Selective preconcentration for capillary zone electrophoresis using protein G immunoaffinity capillary chromatography , 1995, Electrophoresis.

[20]  T. Pritchett,et al.  Capillary electrophoresis-based immunoassays , 1995 .

[21]  T. Scheper,et al.  Analysis of immunoglobulin G using a capillary electrophoretic affinity assay with protein A and laser‐induced fluorescence detection , 1995, Electrophoresis.

[22]  W. Heineman,et al.  Capillary Enzyme Immunoassay with Electrochemical Detection for the Determination of Atrazine in Water , 1995 .

[23]  H. Yamazaki,et al.  A novel immunofluorescence capillary electrophoresis assay system for the determination of chloramphenicol in milk , 1994 .

[24]  M. Lee,et al.  Analysis of monoclonal antibody chimeric BR96-doxorubicin immunoconjugate by sodium dodecyl sulfate-capillary gel electrophoresis with ultraviolet and laser-induced fluorescence detection. , 1995, Analytical biochemistry.

[25]  I. Krull,et al.  Characterization of immunochemical reaction for human growth hormone with its monoclonal antibody by perfusion protein G affinity chromatography and capillary zone electrophoresis. , 1996, Biomedical chromatography : BMC.

[26]  T. Okuyama,et al.  Capillary electrophoresis of immunoglobulin using flat capillary tubing , 1991 .

[27]  D. Schmalzing,et al.  Solution-phase immunoassay for determination of cortisol in serum by capillary electrophoresis. , 1995, Clinical chemistry.

[28]  F. Regnier,et al.  Ultramicro enzyme assays in a capillary electrophoretic system. , 1992, Journal of chromatography.

[29]  W. Heineman,et al.  Electrochemical enzyme immunoassay using sequential saturation technique in a 20-μl capillary: digoxin as a model analyte , 1994 .

[30]  R. Freitag,et al.  Fluorescein isothiocyanate-labeled protein G as an affinity ligand in affinity/immunocapillary electrophoresis with fluorescence detection. , 1994, Analytical chemistry.

[31]  W. Thormann,et al.  Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum , 1995, Electrophoresis.

[32]  K. Nakamura,et al.  Separation of monoclonal antibodies from antihapten antisera by two-dimensional affinity electrophoresis. , 1992, Journal of Chromatography A.

[33]  R. Evangelista,et al.  Feasibility studies for simultaneous immunochemical multianalyte drug assay by capillary electrophoresis with laser-induced fluorescence. , 1994, Clinical chemistry.

[34]  F. Regnier,et al.  Capillary electrophoresis-based immunoassay for cortisol in serum. , 1995, Analytical chemistry.

[35]  D. Schmalzing,et al.  Microchip electrophoretic immunoassay for serum cortisol. , 1996, Analytical chemistry.

[36]  S. Charman,et al.  Analysis of bovine immunoglobulin G by capillary gel electrophoresis. , 1994, Journal of pharmaceutical and biomedical analysis.

[37]  R. Kennedy,et al.  Capillary electrophoresis-based immunoassay to determine insulin content and insulin secretion from single islets of Langerhans. , 1995, Analytical chemistry.

[38]  S. Michaelsen,et al.  High-performance capillary electrophoresis for the determination of trypsin and chymotrypsin inhibitors and their association with trypsin, chymotrypsin and monoclonal antibodies. , 1993, Journal of chromatography. A.

[39]  T. Phillips,et al.  Immunoaffinity capillary electrophoretic analysis of cyclosporin in tears. , 1994, Biomedical chromatography : BMC.

[40]  X. Liu,et al.  Capillary electrophoretic enzyme immunoassay for digoxin in human serum. , 1995, Analytical chemistry.

[41]  N H Heegaard,et al.  Determination of antigen-antibody affinity by immunocapillary electrophoresis. , 1994, Journal of chromatography. A.

[42]  R T Kennedy,et al.  On-line competitive immunoassay for insulin based on capillary electrophoresis with laser-induced fluorescence detection. , 1996, Analytical chemistry.

[43]  S. Pentoney,et al.  Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence. Application to homogeneous digoxin immunoassay. , 1994, Journal of chromatography. A.