Evaluation of procedures to acclimate a microbial fuel cell for electricity production

A microbial fuel cell (MFC) is a relatively new type of fixed film bioreactor for wastewater treatment, and the most effective methods for inoculation are not well understood. Various techniques to enrich electrochemically active bacteria on an electrode were therefore studied using anaerobic sewage sludge in a two-chambered MFC. With a porous carbon paper anode electrode, 8 mW/m2 of power was generated within 50 h with a Coulombic efficiency (CE) of 40%. When an iron oxide-coated electrode was used, the power and the CE reached 30 mW/m2 and 80%, respectively. A methanogen inhibitor (2-bromoethanesulfonate) increased the CE to 70%. Bacteria in sludge were enriched by serial transfer using a ferric iron medium, but when this enrichment was used in a MFC the power was lower (2 mW/m2) than that obtained with the original inoculum. By applying biofilm scraped from the anode of a working MFC to a new anode electrode, the maximum power was increased to 40 mW/m2. When a second anode was introduced into an operating MFC the acclimation time was not reduced and the total power did not increase. These results suggest that these active inoculating techniques could increase the effectiveness of enrichment, and that start up is most successful when the biofilm is harvested from the anode of an existing MFC and applied to the new anode.

[1]  C. Criddle,et al.  The impact of fermentative organisms on carbon flow in methanogenic systems under constant low-substrate conditions , 2001, Applied Microbiology and Biotechnology.

[2]  J L Stirling,et al.  Anodic reactions in microbial fuel cells , 1983, Biotechnology and bioengineering.

[3]  D. R. Bond,et al.  Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments , 2002, Science.

[4]  Bruce E Logan,et al.  Extracting hydrogen and electricity from renewable resources. , 2004, Environmental science & technology.

[5]  L. B. Wingard,et al.  Bioelectrochemical fuel cells , 1982 .

[6]  Byung Hong Kim,et al.  Direct electrode reaction of Fe(III)-reducing bacterium, Shewanella putrefaciens , 1999 .

[7]  W. Verstraete,et al.  Biofuel Cells Select for Microbial Consortia That Self-Mediate Electron Transfer , 2004, Applied and Environmental Microbiology.

[8]  Byung Hong Kim,et al.  Enrichment of microbial community generating electricity using a fuel-cell-type electrochemical cell , 2004, Applied Microbiology and Biotechnology.

[9]  D. Lovley,et al.  Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells , 2003, Nature Biotechnology.

[10]  Amnat Chidthaisong,et al.  Specificity of chloroform, 2-bromoethanesulfonate and fluoroacetate to inhibit methanogenesis and other anaerobic processes in anoxic rice field soil , 2000 .

[11]  L. Stookey.  Ferrozine---a new spectrophotometric reagent for iron , 1970 .

[12]  Byung Hong Kim,et al.  A microbial fuel cell type lactate biosensor using a metal-reducing bacterium, Shewanella putrefaciens , 1999 .

[13]  Andrew D. Wheatley,et al.  Anaerobic digestion : a waste treatment technology , 1990 .

[14]  D. Fortin,et al.  Quantitative characterization of biotic iron oxides by analytical electron microscopy , 2002 .

[15]  M. Wagner,et al.  Abundance and Phylogenetic Affiliation of Iron Reducers in Activated Sludge as Assessed by Fluorescence In Situ Hybridization and Microautoradiography , 2002, Applied and Environmental Microbiology.

[16]  Hong Liu,et al.  Production of electricity during wastewater treatment using a single chamber microbial fuel cell. , 2004, Environmental science & technology.

[17]  D. Lovley,et al.  Organic Matter Mineralization with Reduction of Ferric Iron in Anaerobic Sediments , 1986, Applied and environmental microbiology.

[18]  A. Spormann,et al.  Biochemistry of acetate catabolism in anaerobic chemotrophic bacteria. , 1989, Annual review of microbiology.

[19]  C. Leang,et al.  OmcB, a c-Type Polyheme Cytochrome, Involved in Fe(III) Reduction in Geobacter sulfurreducens , 2003, Journal of bacteriology.

[20]  Kelly P. Nevin,et al.  Mechanisms for Accessing Insoluble Fe(III) Oxide during Dissimilatory Fe(III) Reduction by Geothrix fermentans , 2002, Applied and Environmental Microbiology.

[21]  Largus T Angenent,et al.  Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste. , 2002, Water research.

[22]  Hong Liu,et al.  Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. , 2004, Environmental science & technology.

[23]  Bruce E. Logan,et al.  Microbial Fuel Cells , 2006 .

[24]  S. Chaturvedi,et al.  Protein S Deficiency, Activated Protein C Resistance and Sticky Platelet Syndrome in a Young Woman with Bilateral Strokes , 1999, Cerebrovascular Diseases.

[25]  D. Lovley,et al.  Characterization of a membrane-bound NADH-dependent Fe(3+) reductase from the dissimilatory Fe(3+)-reducing bacterium Geobacter sulfurreducens. , 2000, FEMS microbiology letters.

[26]  Baikun Li,et al.  Bacterial adhesion to glass and metal-oxide surfaces. , 2004, Colloids and surfaces. B, Biointerfaces.

[27]  P. McDermott,et al.  Characterization of Multiple-Antimicrobial-Resistant Salmonella Serovars Isolated from Retail Meats , 2004, Applied and Environmental Microbiology.

[28]  Hong Liu,et al.  Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell. , 2005, Environmental science & technology.

[29]  Bruce E Logan,et al.  Cathode performance as a factor in electricity generation in microbial fuel cells. , 2004, Environmental science & technology.

[30]  D. Lovley,et al.  Novel Mode of Microbial Energy Metabolism: Organic Carbon Oxidation Coupled to Dissimilatory Reduction of Iron or Manganese , 1988, Applied and environmental microbiology.

[31]  Byung Hong Kim,et al.  Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses. , 2003, FEMS microbiology letters.

[32]  P. Chiu,et al.  2-Bromoethanesulfonate Affects Bacteria in a Trichloroethene-Dechlorinating Culture , 2001, Applied and Environmental Microbiology.

[33]  D. R. Bond,et al.  Electricity Production by Geobacter sulfurreducens Attached to Electrodes , 2003, Applied and Environmental Microbiology.