Speckle interferometry applied to pharmacodynamic studies: evaluation of parasite motility

The work reported here describes the application of the optical technique known as dynamic speckle interferometry to evaluate the motility of nematode parasites exposed to different anthelmintic drugs. This technique, a well proven tool for assessing the time evolution of different phenomena, is here successfully used to quantify parasite motility in pharmacodynamic assays. The characterization of the pharmacological properties of anthelmintic drugs is critical to optimize their use in parasite control. Besides, the evaluation of nematode motility is a relevant indicator of the pharmacodynamic effect of anthelmintic drugs. The application of this approach to study the motility of Haemonchus contortus (used as a model of nematode parasites) larvae exposed to different drugs is presented, showing its usefulness.

[1]  Héctor Rabal,et al.  Bio-speckle assessment of bruising in fruits , 2003 .

[2]  R. Martin,et al.  Modes of action of anthelmintic drugs. , 1997, Veterinary journal.

[3]  G. Tribillon,et al.  Biological Activity Measurement on Botanical Specimen Surfaces Using a Temporal Decorrelation Effect of Laser Speckle , 1989 .

[4]  C. Friis,et al.  A new in vitro assay of benzimidazole activity against adult Oesophagostomum dentatum. , 1997, International journal for parasitology.

[5]  Héctor Rabal,et al.  Display of local activity using dynamical speckle patterns , 2002 .

[6]  Yoshihisa Aizu,et al.  2 – Bio-speckles , 1996 .

[7]  J. D. Baggot,et al.  The absorption, distribution and elimination of anthelmintic drugs: the role of pharmacokinetics. , 1994, Journal of veterinary pharmacology and therapeutics.

[8]  T. Geary,et al.  Mechanistic approaches to quantitate anthelmintic absorption by gastrointestinal nematodes. , 1993, Parasitology today.

[9]  P. Martin,et al.  Larval paralysis as an in vitro assay of levamisole and morantel tartrate resistance inOstertagia , 2005, Veterinary Science Communications.

[10]  R. Sirohi.  Speckle Metrology , 1993, Optical Methods of Measurement.

[11]  J. Dainty.  Laser speckle and related phenomena , 1975 .

[12]  Héctor Rabal,et al.  Speckle time evolution characterization by the co-occurrence matrix analysis , 1999 .

[13]  F. Borgsteede,et al.  Facts and reflections IV. Resistance of parasites to anthelmintics. A workshop in the C.E.C. animal pathology programme held at the Central Veterinary Institute, Lelystad, The Netherlands, 9-10 December 1982. Sponsored by the Commission of the European Communities. , 1983 .

[14]  J. Walley.  Tetramisole (dl 2,3,5,6-tetrahydro-6-phenyl-imidazo (2,1-b) thiazole hydrochloride--Nilverm) in the treatment of gastro-intestinal worms and lungworms in domestic animals. 1. Sheep and goats , 1966, Veterinary Record.

[15]  G. Bourdoiseau.  Resistance to anthelmintics , 1992 .

[16]  M Adel,et al.  Fast algorithm for texture discrimination by use of a separable orthonormal decomposition of the co-occurrence matrix. , 1997, Applied optics.