Dual-Analyte Sensing with a Molecularly Imprinted Polymer Based on Enhancement-Mode Organic Electrochemical Transistors.

Novel enhancement-mode organic electrochemical transistors (OECTs) have been prepared by poly(3, 4-ethylenedioxythiophene)-poly(styrenesulfonate) de-doped polyethylenimine on the multi-walled carbon nanotube-modified viscose yarn. The fabricated devices exhibit low power consumption with a high transconductance of 6.7 mS, rapid response time < 2 s, and excellent cyclic stability. In addition, the device has washing durability and bending and long-term stability suitable for wearable applications. Biosensors based on enhancement-mode OECTs for the selective detection of adrenaline and uric acid (UA) are developed by using molecularly imprinted polymer (MIP)-functionalized gate electrodes. The detection limits of adrenaline and UA analysis are as low as 1 pM, with the linear ranges of 0.5 pM to 10 μM and 1 pM to 1 mM, respectively. Moreover, the sensor based on enhancement-mode transistors can efficiently amplify the current signals according to the modulation of the gate voltage. The MIP-modified biosensor has high selectivity in the presence of interferents and desirable reproducibility. Additionally, due to the wearable nature of the developed biosensor, this sensing tool has the capability of being integrated with fabrics. Therefore, it has successfully been applied in textiles for the determination of adrenaline and UA in artificial urine samples. The excellent recoveries and rsds are 90.22-109.05% and 3.97-6.94%, respectively. Ultimately, these sensitive, low-power, wearable, and dual-analyte sensors help to develop non-laboratory tools for early disease diagnosis and clinical research.

[1]  M. Brandl,et al.  Layer-by-layer sensor architecture of polymers and nanoparticles for electrochemical detection of uric acid in human urine samples , 2021, Materials Today Chemistry.

[2]  G. Aiello,et al.  Electrochemical detection of uric acid and ascorbic acid using r-GO/NPs based sensors , 2021, Electrochimica Acta.

[3]  O. Kwon,et al.  Carbon fiber coating with MWCNT in the presence of polyethyleneimine of different molecular weights and the effect on the interfacial shear strength of thermoplastic and thermosetting carbon fiber composites , 2020, Carbon Letters.

[4]  A. Salleo,et al.  Enhancement‐Mode PEDOT:PSS Organic Electrochemical Transistors Using Molecular De‐Doping , 2020, Advanced materials.

[5]  George D. Spyropoulos,et al.  Enhancement-mode ion-based transistor as a comprehensive interface and real-time processing unit for in vivo electrophysiology , 2020, Nature Materials.

[6]  M. Thelakkat,et al.  The Key Role of Side Chain Linkage in Structure Formation and Mixed Conduction of Ethylene Glycol Substituted Polythiophenes. , 2020, ACS applied materials & interfaces.

[7]  A. Sag,et al.  The Effect of Urine pH and Urinary Uric Acid Levels on the Development of Contrast Nephropathy , 2019, Kidney and Blood Pressure Research.

[8]  Ying Sun,et al.  Layer-by-layer self-assembly film of PEI-reduced graphene oxide composites and cholesterol oxidase for ultrasensitive cholesterol biosensing , 2019, Sensors and Actuators B: Chemical.

[9]  A. Salleo,et al.  Mechanisms for Enhanced State Retention and Stability in Redox‐Gated Organic Neuromorphic Devices , 2018, Advanced Electronic Materials.

[10]  Armantas Melianas,et al.  Organic electronics for neuromorphic computing , 2018, Nature Electronics.

[11]  T. Jaramillo,et al.  A Universal Platform for Fabricating Organic Electrochemical Devices , 2018, Advanced Electronic Materials.

[12]  X. Crispin,et al.  Complementary Logic Circuits Based on High‐Performance n‐Type Organic Electrochemical Transistors , 2018, Advanced materials.

[13]  M. Marinella,et al.  A non-volatile organic electrochemical device as a low-voltage artificial synapse for neuromorphic computing. , 2017, Nature materials.

[14]  J. Wilson,et al.  Enzymeless biosensor based on β-NiS@rGO/Au nanocomposites for simultaneous detection of ascorbic acid, epinephrine and uric acid , 2016 .

[15]  Aram Amassian,et al.  N-type organic electrochemical transistors with stability in water , 2016, Nature Communications.

[16]  Zhihui Dai,et al.  An Improved Ultrasensitive Enzyme-Linked Immunosorbent Assay Using Hydrangea-Like Antibody-Enzyme-Inorganic Three-in-One Nanocomposites. , 2016, ACS applied materials & interfaces.

[17]  H. Al‐Lohedan,et al.  The synthesis of desired functional groups on PEI microgel particles for biomedical and environmental applications , 2015 .

[18]  Jing Guo,et al.  Rigid and Flexible Organic Electrochemical Transistor Arrays for Monitoring Action Potentials from Electrogenic Cells , 2015, Advanced healthcare materials.

[19]  X. Crispin,et al.  Poly(ethylene imine) Impurities Induce n‐doping Reaction in Organic (Semi)Conductors , 2014, Advanced materials.

[20]  P. Leleux,et al.  High transconductance organic electrochemical transistors , 2013, Nature Communications.

[21]  Wei Wang,et al.  Analysis of the interaction between tropomyosin allergens and antibodies using a biosensor based on imaging ellipsometry. , 2013, Analytical chemistry.

[22]  Z. Dursun,et al.  Simultaneous determination of ascorbic acid, epinephrine and uric acid at over-oxidized poly(p-aminophenol) film modified electrode , 2013 .

[23]  P. Leleux,et al.  In vivo recordings of brain activity using organic transistors , 2013, Nature Communications.

[24]  Zhifeng Fu,et al.  Highly sensitive trivalent copper chelate-luminol chemiluminescence system for capillary electrophoresis detection of epinephrine in the urine of smoker. , 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[25]  Shoufang Xu,et al.  Recent advances in molecular imprinting technology: current status, challenges and highlighted applications. , 2011, Chemical Society reviews.

[26]  R. Penner,et al.  Enhanced thermoelectric metrics in ultra-long electrodeposited PEDOT nanowires. , 2011, Nano letters.

[27]  George G. Malliaras,et al.  Influence of Device Geometry on Sensor Characteristics of Planar Organic Electrochemical Transistors , 2010, Advanced materials.

[28]  S. A. John,et al.  Selective electrochemical epinephrine sensor using self-assembled monomolecular film of 1,8,15,22-tetraaminophthalocyanatonickel(II) on gold electrode , 2008 .

[29]  Lei Ye,et al.  Molecular imprinting: Synthetic materials as substitutes for biological antibodies and receptors , 2008 .

[30]  Rongxiu Li,et al.  Development and characterisation of molecularly imprinted polymers based on methacrylic acid for selective recognition of drugs. , 2007, Biomaterials.

[31]  D.A. Johns,et al.  Analog integrated circuit design [Book Review] , 2000, IEEE Circuits and Devices Magazine.

[32]  P. Hernández,et al.  Cyclic voltammetry determination of epinephrine with a carbon fiber ultramicroelectrode. , 1998, Talanta.

[33]  Klaus Mosbach,et al.  Drug assay using antibody mimics made by molecular imprinting , 1993, Nature.

[34]  Jingkun Xu,et al.  Fabrication of PEDOT:PSS/rGO fibers with high flexibility and electrochemical performance for supercapacitors , 2021, Electrochimica Acta.

[35]  K. Tadi,et al.  Interfacing Electrochemically Reduced Graphene Oxide with Poly(erichrome black T) for Simultaneous Determination of Epinephrine, Uric Acid and Folic Acid , 2018 .

[36]  R. Goyal,et al.  Electrochemical and peroxidase-catalyzed oxidation of epinephrine , 2012 .

[37]  B. Halliwell,et al.  Action of biologically-relevant oxidizing species upon uric acid. Identification of uric acid oxidation products. , 1990, Chemico-biological interactions.