Design of a CNFET array for sensing and control in P450 based biochips for multiple drug detection

Bio-detection specially dedicated to distributed diagnostics is emerging as a quite important application for Nano-bioelectronics. Bio-detection is required to be highly sensitive in order to succeed in sensing small amount of bio-markers in patient's blood sample. Carbon Nanotubes (CNTs) provides devices in the scale of the target molecules, thereby opening up possibilities to sense few bio-markers. Moreover, bio-detection is also required to be highly specific in order to succeed in disease discrimination. FET technology provides control in multi-panel biochip to enhance specificity. The aim of the present paper is to design an array of Carbon Nanotube Field Effect Transistors (CNFETs) to provide nano-biosensing based on cytochromes P450. In particular, a novel CNFET biosensor array design is proposed which is robust to the imperfections in the CNTs thereby achieving high level of sensitivity. The proposed CNFET based design assures the improved specificity by means of a multi-gate controller at the nano-scale. The proposed application in distributed diagnostics is on detection of drugs in multi-components samples by multiplexing different P450 probes.

[1]  Jiunn H Lin,et al.  Pharmacokinetic and pharmacodynamic variability: a daunting challenge in drug therapy. , 2007, Current drug metabolism.

[2]  J. Miners,et al.  Electrochemical characterisation of the human cytochrome P450 CYP2C9. , 2005, Biochemical pharmacology.

[3]  Evan Shave,et al.  Preparative-scale isoelectric trapping separations using a modified Gradiflow unit. , 2002, Journal of chromatography. A.

[4]  A. Daly,et al.  Individualized drug therapy. , 2007, Current opinion in drug discovery & development.

[5]  G. Grüner,et al.  Integration of cell membranes and nanotube transistors. , 2005, Nano letters.

[6]  R. Martel,et al.  Carbon nanotube field effect transistors - fabrication, device physics, and circuit implications , 2003, 2003 IEEE International Solid-State Circuits Conference, 2003. Digest of Technical Papers. ISSCC..

[7]  Edward H. Kerns,et al.  Editorial [Hot Topic: High Throughput In Vitro ADME/Tox Profiling for Drug Discovery(Guest Editor: Edward H. Kerns)] , 2008 .

[8]  J. Rogers,et al.  High-performance electronics using dense, perfectly aligned arrays of single-walled carbon nanotubes. , 2007, Nature nanotechnology.

[9]  Danielson Pb,et al.  The cytochrome P450 superfamily: biochemistry, evolution and drug metabolism in humans. , 2002 .

[10]  P. Corey,et al.  Incidence of Adverse Drug Reactions in Hospitalized Patients , 2012 .

[11]  Qian Wang,et al.  Toward Large Arrays of Multiplex Functionalized Carbon Nanotube Sensors for Highly Sensitive and Selective Molecular Detection. , 2003, Nano letters.

[12]  Sandro Carrara,et al.  Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors. , 2008, Biosensors & bioelectronics.

[13]  P. Avouris,et al.  Engineering Carbon Nanotubes and Nanotube Circuits Using Electrical Breakdown , 2001, Science.

[14]  U. Fuhr,et al.  Evaluation of probe drugs and pharmacokinetic metrics for CYP2D6 phenotyping , 2007, European Journal of Clinical Pharmacology.

[15]  George Grüner.  Carbon nanotube transistors for biosensing applications. , 2005 .