论文信息 - Five‐membered mercaptoheterocyclic ligands for thiophilic adsorption chromatography

Five‐membered mercaptoheterocyclic ligands for thiophilic adsorption chromatography

A series of five‐membered mercaptoheterocyles containing at least two heteroatoms in their ring structure was investigated for their ability to specifically adsorb antibodies. The structures that contain at least one double bond adsorbed antibodies, while the saturated structure showed no protein interaction under the experimental conditions studied. The influence of various lyotropic salt on adsorbance was studied.

A. Schwarz

[1] M. Wilchek,et al. Novel heterocyclic ligands for the thiophilic purification of antibodies. , 1995, Journal of chromatography. B, Biomedical applications.

[2] J. Porath,et al. Amphiphilic agarose-based adsorbents for chromatography. Comparative study of adsorption capacities and desorption efficiencies. , 1995, Journal of chromatography. A.

[3] M. Wilchek,et al. Novel sulfone-based thiophilic ligands for the high-performance liquid chromatographic purification of antibodies☆ , 1994 .

[4] L. Franco-Fraguas,et al. Thiophilic interaction chromatography of sweet potato β-amylase , 1992 .

[5] K. Knudsen,et al. Sulfone-aromatic ligands for thiophilic adsorption chromatography: purification of human and mouse immunoglobulins. , 1992, Analytical biochemistry.

[6] M. Wilchek,et al. A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies. , 1989, Analytical biochemistry.

[7] J. Porath,et al. A new kind of thiophilic electron-donor-acceptor adsorbent , 1988 .

[8] J. Porath,et al. Thiophilic adsorption ‐ a new method for protein fractionation , 1985, FEBS letters.