High-speed widefield photoacoustic microscopy of small-animal hemodynamics.

Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest.

[1]  Junjie Yao,et al.  Fast voice-coil scanning optical-resolution photoacoustic microscopy. , 2011, Optics letters.

[2]  Vasilis Ntziachristos,et al.  Hybrid multiphoton and optoacoustic microscope. , 2014, Optics letters.

[3]  Vasilis Ntziachristos,et al.  Looking and listening to light: the evolution of whole-body photonic imaging , 2005, Nature Biotechnology.

[4]  Qifa Zhou,et al.  Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution. , 2011, Optics letters.

[5]  P. Kadowitz,et al.  Studies on the mechanism of hydrocortisone potentiation of vasoconstrictor responses to epinephrine in the anesthetized animal , 1972 .

[6]  A. J. Jerri.  The Shannon sampling theorem—Its various extensions and applications: A tutorial review , 1977, Proceedings of the IEEE.

[7]  Jin Young Kim,et al.  Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner , 2015, Scientific Reports.

[8]  Vasilis Ntziachristos,et al.  Fast scanning coaxial optoacoustic microscopy , 2012, Biomedical optics express.

[9]  Lihong V. Wang,et al.  High-speed label-free functional photoacoustic microscopy of mouse brain in action , 2015, Nature Methods.

[10]  Junjie Yao,et al.  Photoacoustic microscopy , 2013, Laser & photonics reviews.

[11]  V. Ntziachristos,et al.  Video rate optoacoustic tomography of mouse kidney perfusion. , 2010, Optics letters.

[12]  Ryan L Shelton,et al.  Volumetric imaging of erythrocytes using label-free multiphoton photoacoustic microscopy. , 2014, Journal of biophotonics.

[13]  R. Lockey,et al.  Epinephrine: the drug of choice for anaphylaxis. A statement of the World Allergy Organization , 2008, Allergy.

[14]  Hao Zhang,et al.  Imaging of hemoglobin oxygen saturation variations in single vessels in vivo using photoacoustic microscopy , 2007 .

[15]  Fei Gao,et al.  Advanced photoacoustic and thermoacoustic sensing and imaging beyond pulsed absorption contrast , 2016 .

[16]  A. Nimmerjahn,et al.  Stepwise Recruitment of Transcellular and Paracellular Pathways Underlies Blood-Brain Barrier Breakdown in Stroke , 2014, Neuron.

[17]  A. Abbasi,et al.  Effect of different epinephrine concentrations on local bleeding and hemodynamics during dermatologic surgery. , 2008, Acta dermatovenerologica Croatica : ADC.

[18]  Vasilis Ntziachristos,et al.  Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging. , 2009, Optics express.

[19]  J. Engdahl,et al.  Drug therapy in cardiac arrest: a review of the literature. , 2016, European heart journal. Cardiovascular pharmacotherapy.

[20]  Daniel Razansky,et al.  Real-time optoacoustic brain microscopy with hybrid optical and acoustic resolution , 2014 .

[21]  Carmen A. Puliafito,et al.  Laser-scanning optical-resolution photoacoustic microscopy , 2009, BiOS.

[22]  D. Hermann,et al.  Post-acute delivery of memantine promotes post-ischemic neurological recovery, peri-infarct tissue remodeling, and contralesional brain plasticity , 2017, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[23]  Lihong V Wang,et al.  Photoacoustic tomography and sensing in biomedicine , 2009, Physics in medicine and biology.

[24]  Lei Xi,et al.  Large-field-of-view optical resolution photoacoustic microscopy. , 2018, Optics express.

[25]  Vasilis Ntziachristos,et al.  Multispectral Opto-acoustic Tomography (MSOT) of the Brain and Glioblastoma Characterization , 2013, NeuroImage.

[26]  Y. Li,et al.  XBP1 (X-Box–Binding Protein-1)–Dependent O-GlcNAcylation Is Neuroprotective in Ischemic Stroke in Young Mice and Its Impairment in Aged Mice Is Rescued by Thiamet-G , 2017, Stroke.

[27]  Lei Xi,et al.  Portable optical resolution photoacoustic microscopy (pORPAM) for human oral imaging. , 2017, Optics letters.

[28]  Lihong V. Wang,et al.  Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging. , 2010, Optics letters.

[29]  Lihong V. Wang,et al.  Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs , 2012, Science.

[30]  Jin Young Kim,et al.  High-speed and high-SNR photoacoustic microscopy based on a galvanometer mirror in non-conducting liquid , 2016, Scientific Reports.

[31]  J. Laufer,et al.  In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy , 2009, Physics in medicine and biology.

[32]  F. Abboud,et al.  Epinephrine facilitates neurogenic vasoconstriction in humans. , 1988, The Journal of clinical investigation.

[33]  P. Bartolomeo,et al.  Right spatial neglect after left hemisphere stroke , 2004, Neurology.

[34]  Lihong V. Wang,et al.  Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries. , 2008, Optics letters.

[35]  M. Intaglietta,et al.  Variations of rhythmic diameter changes at the arterial microvascular bifurcations , 1985, Pflügers Archiv.

[36]  Wei Shi,et al.  In vivo near-realtime volumetric optical-resolution photoacoustic microscopy using a high-repetition-rate nanosecond fiber-laser. , 2011, Optics express.

[37]  Jan Laufer,et al.  Three-dimensional noninvasive imaging of the vasculature in the mouse brain using a high resolution photoacoustic scanner. , 2009, Applied optics.

[38]  Oxana V. Baranova,et al.  Neuron-Specific Inactivation of the Hypoxia Inducible Factor 1α Increases Brain Injury in a Mouse Model of Transient Focal Cerebral Ischemia , 2007, The Journal of Neuroscience.

[39]  Jun Zou,et al.  Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror. , 2012, Journal of biomedical optics.

[40]  Zhixing Xie,et al.  Laser-scanning optical-resolution photoacoustic microscopy. , 2009, Optics letters.

[41]  Lihong V. Wang,et al.  Second generation optical-resolution photoacoustic microscopy , 2011, BiOS.

[42]  Vasilis Ntziachristos,et al.  Hybrid optical and acoustic resolution optoacoustic endoscopy. , 2016, Optics letters.

[43]  Junjie Yao,et al.  Sensitivity of photoacoustic microscopy , 2014, Photoacoustics.