Formulation and Evaluation of Cat Fish Slim Mucin Ointment for Wound Healing

Purpose: To evaluate the effect of fish mucin ointment on wound healing in a rat model. Methods : Fish mucin was formulated into an ointment using soft paraffin ointment base. Its woundhealing activity and toxicity were evaluated using an incision and excision wound model in rats. A range of concentrations (2.5 - 10 % w/w) of mucin in the ointment was tested to determine the concentration that will give optimum wound healing activity The ointment base (i.e., without mucin) was used as control while 2 % nitrofurazone served as positive control. Results : Wound healing activity increased with increase in mucin concentration up to a maximum of 5 %. Negative control produced wound healing of 41.2 ± 0.0 % on the 19 th day with the mean hydroxyproline content and tensile strength being 682.07 ± 0.11 µg/g and 874.11 ± 0.39 g/cm 2 , respectively. Mucin ointment (5 %) exhibited 100 % wound healing properties on day 12. Significant increase (p > 0.05) in skin tensile strength (1311.02 ± 0.16 g/cm 2 ) and hydroxyproline (1163.11± 0.16 µg/g) was found for mucin ointment as against 1151.21 ± 0.41 g/cm 2 and 875.12 ± 0.84 µg/g, respectively, for positive control. At the concentrations tested, no toxicity was recorded for mucin ointment. Conclusion: This study demonstrates that the fish mucin ointment promotes wound-healing.

[1]  M. Momoh,et al.  In vitro Evaluation of PEGylated-Mucin Matrix as Carrier for Oral Delivery of Metformin Hydrochloride , 2014 .

[2]  Huige Li,et al.  Neuroprotective and Antioxidative Effect of Cactus Polysaccharides In Vivo and In Vitro , 2009, Cellular and Molecular Neurobiology.

[3]  M. Momoh,et al.  The Effect of Cast Films of Bovine Mucin and Prosopis Gum Containing a Cicatrin® Powder on Wound Healing , 2008 .

[4]  A. Mittal,et al.  Glycoproteins in the Epithelium of Lips and Associated Structures of a Hill Stream Fish Garra lamta (Cyprinidae, Cypriniformes): A Histochemical Investigation , 2008, Anatomia, histologia, embryologia.

[5]  M. Adikwu,et al.  Application of snail mucin dispersed in detarium gum gel in wound healing , 2007 .

[6]  A. Corfield,et al.  Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal disease , 2000, Gut.

[7]  O. Thulesius,et al.  Multiple vasoactive factors in epidermal secretions of the Arabian Gulf catfish, Arius bilineatus (Valenciennes). , 1997, General pharmacology.

[8]  P. Mukherjee,et al.  Wound healing activity of Leucas lavandulaefolia Rees. , 1997, Journal of ethnopharmacology.

[9]  R. Gruen,et al.  Optimizing the hospital management of leg ulcers. , 1996, The Australian and New Zealand journal of surgery.

[10]  S. Mortazavi,et al.  A comparative study on the role played by mucus glycoproteins in the rheological behaviour of the mucoadhesive/mucosal interface , 1993 .

[11]  M. Sigel,et al.  IgM Antibodies in Fish Mucus 1 , 1971, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[12]  J. Prudden,et al.  THE CLINICAL ACCELERATION OF HEALING WITH A CARTILAGE PREPARATION; A CONTROLLED STUDY. , 1965, JAMA.

[13]  G. Winter,et al.  Formation of the Scab and the Rate of Epithelization of Superficial Wounds in the Skin of the Young Domestic Pig , 1962, Nature.

[14]  J. Al-Hassan Diabetic ulcer healing preparations from the skin of the Arabian Gulf catfish (Arius bilineatus Val.): a novel and effective treatment. , 1990, International journal of tissue reactions.