A nanostructured conductive hydrogels-based biosensor platform for human metabolite detection.

The development of a scalable, low-cost, and versatile biosensor platform for the sensitive and rapid detection of human metabolites is of great interest for healthcare, pharmaceuticals, and medical science. On the basis of hierarchically nanostructured conducting polymer hydrogels, we designed a flexible biosensor platform that can detect various human metabolites, such as uric acid, cholesterol, and triglycerides. Owing to the unique features of conducting polymer hydrogels, such as high permeability to biosubstrates and rapid electron transfer, our biosensors demonstrate excellent sensing performance with a wide linear range (uric acid, 0.07-1 mM; cholesterol, 0.3-9 mM, and triglycerides, 0.2-5 mM), high sensitivity, low sensing limit, and rapid response time (∼3 s). Given the facile and scalable processability of hydrogels, the proposed conductive hydrogels-based biosensor platform shows great promise as a low-cost sensor kit for healthcare monitoring, clinical diagnostics, and biomedical devices.

[1]  G. Guan,et al.  A novel electroactive hybrid film electrode with proton buffer effect for detecting hydrogen peroxide and uric acid , 2014 .

[2]  Arnaud Magrez,et al.  High-performance multipanel biosensors based on a selective integration of nanographite petals. , 2014, Nano letters.

[3]  Xiaoquan Lu,et al.  Simultaneous determination of ascorbic acid, dopamine and uric acid based on tryptophan functionalized graphene. , 2014, Analytica chimica acta.

[4]  Proespichaya Kanatharana,et al.  An amperometric uric acid biosensor based on chitosan-carbon nanotubes electrospun nanofiber on silver nanoparticles , 2014, Analytical and Bioanalytical Chemistry.

[5]  P. Xu,et al.  Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides. , 2014, Biosensors & bioelectronics.

[6]  S. Srivastava,et al.  A chitosan-based polyaniline–Au nanocomposite biosensor for determination of cholesterol , 2014 .

[7]  C. Pundir,et al.  Determination of triglycerides with special emphasis on biosensors: a review. , 2013, International journal of biological macromolecules.

[8]  Saurabh Srivastava,et al.  Highly Efficient Bienzyme Functionalized Nanocomposite-Based Microfluidics Biosensor Platform for Biomedical Application , 2013, Scientific Reports.

[9]  Lijia Pan,et al.  3D nanostructured conductive polymer hydrogels for high-performance electrochemical devices , 2013 .

[10]  C. Pundir,et al.  Construction of triglyceride biosensor based on nickel oxide-chitosan/zinc oxide/zinc hexacyanoferrate film. , 2013, International journal of biological macromolecules.

[11]  Meihe Zhang,et al.  Cerium oxide-graphene as the matrix for cholesterol sensor. , 2013, Analytical biochemistry.

[12]  Rong Zhang,et al.  Highly sensitive glucose sensor based on pt nanoparticle/polyaniline hydrogel heterostructures. , 2013, ACS nano.

[13]  A. Tuantranont,et al.  A disposable amperometric biosensor based on inkjet-printed Au/PEDOT-PSS nanocomposite for triglyceride determination , 2013 .

[14]  B. Thakur,et al.  Polyaniline/Prussian‐Blue‐Based Amperometric Biosensor for Detection of Uric Acid , 2013 .

[15]  S. Penner,et al.  Metal–Support Interaction in Pt/VOx and Pd/VOx Systems: A Comparative (HR)TEM Study , 2013, Catalysis Letters.

[16]  C. Pundir,et al.  Construction of an amperometric TG biosensor based on AuPPy nanocomposite and poly (indole-5-carboxylic acid) modified Au electrode , 2013, Bioprocess and Biosystems Engineering.

[17]  B. D. Malhotra,et al.  A novel ternary NiFe2O4/CuO/FeO-chitosan nanocomposite as a cholesterol biosensor , 2012 .

[18]  Youngkwan Lee,et al.  Nonenzymatic cholesterol sensor based on spontaneous deposition of platinum nanoparticles on layer-by-layer assembled CNT thin film , 2012 .

[19]  Zhenan Bao,et al.  Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity , 2012, Proceedings of the National Academy of Sciences.

[20]  C. Pundir,et al.  Construction of amperometric uric acid biosensor based on uricase immobilized on PBNPs/cMWCNT/PANI/Au composite. , 2012, International journal of biological macromolecules.

[21]  J. Narang,et al.  Construction of a triglyceride amperometric biosensor based on chitosan-ZnO nanocomposite film. , 2011, International journal of biological macromolecules.

[22]  Jian Wang,et al.  Microwave-assisted synthesis of a core-shell MWCNT/GONR heterostructure for the electrochemical detection of ascorbic acid, dopamine, and uric acid. , 2011, ACS nano.

[23]  Aicheng Chen,et al.  High-performance electrochemical biosensor for the detection of total cholesterol. , 2011, Biosensors & bioelectronics.

[24]  B D Malhotra,et al.  Recent advances in polyaniline based biosensors. , 2011, Biosensors & bioelectronics.

[25]  A. Safavi,et al.  Electrodeposition of gold-platinum alloy nanoparticles on ionic liquid-chitosan composite film and its application in fabricating an amperometric cholesterol biosensor. , 2011, Biosensors & bioelectronics.

[26]  C. R. Raj,et al.  Development of an Amperometric Cholesterol Biosensor Based on Graphene-Pt Nanoparticle Hybrid Material , 2010 .

[27]  P. Solanki,et al.  Polyaniline/Single-Walled Carbon Nanotubes Composite Based Triglyceride Biosensor , 2010 .

[28]  C. Pundir,et al.  Fabrication of dissolved O 2 metric uric acid biosensor based on uricase bound to PVC membrane , 2010 .

[29]  Chandra Shekhar Pundir,et al.  Construction of an amperometric enzymic sensor for triglyceride determination , 2008 .

[30]  Joseph Wang.  Electrochemical glucose biosensors. , 2008, Chemical reviews.

[31]  C. R. Raj,et al.  Mercaptoethylpyrazine promoted electrochemistry of redox protein and amperometric biosensing of uric acid. , 2007, Biosensors & bioelectronics.

[32]  B. D. Malhotra,et al.  Dithiobissuccinimidyl propionate self assembled monolayer based cholesterol biosensor. , 2007, The Analyst.

[33]  Martin M. F. Choi,et al.  Development and analytical application of an uric acid biosensor using an uricase-immobilized eggshell membrane. , 2007, Biosensors & bioelectronics.

[34]  C. Liu,et al.  An amperometric uric acid biosensor based on modified Ir-C electrode. , 2006, Biosensors & bioelectronics.

[35]  P. Solanki,et al.  Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor. , 2006, Analytica chimica acta.

[36]  Bansi D. Malhotra,et al.  Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films , 2006 .

[37]  Gengfeng Zheng,et al.  Multiplexed electrical detection of cancer markers with nanowire sensor arrays , 2005, Nature Biotechnology.

[38]  G. Li,et al.  Study of carbon nanotube modified biosensor for monitoring total cholesterol in blood. , 2005, Biosensors & bioelectronics.

[39]  E. García-Ruiz,et al.  Amperometric cholesterol biosensors based on the electropolymerization of pyrrole and the electrocatalytic effect of Prussian-Blue layers helped with self-assembled monolayers. , 2004, Talanta.

[40]  Asha Chaubey,et al.  Application of conducting polymers to biosensors. , 2002, Biosensors & bioelectronics.