Ruthenium-based metal organic framework (Ru-MOF)-derived novel Faraday-cage electrochemiluminescence biosensor for ultrasensitive detection of miRNA-141

Abstract A novel Faraday-cage electrochemiluminescence (ECL) biosensor for the sensitive detection of miRNA-141 was constructed. The capture unit was prepared by immobilizing the capture DNA (cDNA) on the functionalized magnetic nanospheres nanoFe3O4@SiO2@Au, while the signal unit was ruthenium-based metal organic framework Ru-MOF labeled by signal DNA (sDNA). The recognition scaffold was as follows: the capture unit was immobilized onto the magnetic electrode surface; the target miRNA-141 was caught; the signal unit was further hybridized; the Faraday-cage biosensor structure was formed finally. In this case, the signal unit became part of the electrode surface, the outer Helmholtz plane (OHP) of the proposed electrode was extended, all electrochemiluminophores in the signal unit could take part in the electrode reaction, and thus then the detection sensitivity was greatly improved. Taking advantage of the proposed Faraday-cage cascade, the ECL intensity was found to increase with the logarithm of miRNA-141 concentration. The linear range was wide from 1 fM to 10 pM with a limit of detection 0.3 fM. The selectivity, stability, reproducibility, precision and application of this biosensor were validated. This proposed Faraday-cage ECL biosensor has a potential prospect for clinical miRNA detection.

[1]  K. Muzyka.  Current trends in the development of the electrochemiluminescent immunosensors. , 2014, Biosensors & bioelectronics.

[2]  Zhiyong Guo,et al.  In-electrode vs. on-electrode: ultrasensitive Faraday cage-type electrochemiluminescence immunoassay. , 2016, Chemical communications.

[3]  H. Horvitz,et al.  MicroRNA expression profiles classify human cancers , 2005, Nature.

[4]  Jimmy C. Yu,et al.  Preparation, characterization, and catalytic activity of core/shell Fe3O4@polyaniline@au nanocomposites. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[5]  Qian Wang,et al.  Graphene fluorescence switch-based cooperative amplification: a sensitive and accurate method to detection microRNA. , 2014, Analytical chemistry.

[6]  Zhiyong Guo,et al.  Faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of Vibrio vulnificus based on multi-functionalized graphene oxide , 2016, Analytical and Bioanalytical Chemistry.

[7]  Xiaoyan Wu,et al.  An "off-on" electrochemiluminescent biosensor based on DNAzyme-assisted target recycling and rolling circle amplifications for ultrasensitive detection of microRNA. , 2015, Analytical chemistry.

[8]  B. Han,et al.  Ru nanoparticles immobilized on metal–organic framework nanorods by supercritical CO2-methanol solution: highly efficient catalyst , 2011 .

[9]  Guobao Xu,et al.  Applications and trends in electrochemiluminescence. , 2010, Chemical Society reviews.

[10]  Pu Zhang,et al.  Bi-directional DNA Walking Machine and Its Application in an Enzyme-Free Electrochemiluminescence Biosensor for Sensitive Detection of MicroRNAs. , 2017, Analytical chemistry.

[11]  Zhiyong Guo,et al.  Electrochemiluminescence immunosensor for tumor markers based on biological barcode mode with conductive nanospheres. , 2014, Biosensors & bioelectronics.

[12]  Mohammad Rizwan,et al.  Trends and Advances in Electrochemiluminescence Nanobiosensors , 2018, Sensors.

[13]  Y. Chai,et al.  Luminescence-Functionalized Metal-Organic Frameworks Based on a Ruthenium(II) Complex: A Signal Amplification Strategy for Electrogenerated Chemiluminescence Immunosensors. , 2015, Chemistry.

[14]  D. Xing,et al.  Ultrasensitive Detection of MicroRNA in Tumor Cells and Tissues via Continuous Assembly of DNA Probe. , 2015, Biomacromolecules.

[15]  Chad A Mirkin,et al.  Nucleic acid-metal organic framework (MOF) nanoparticle conjugates. , 2014, Journal of the American Chemical Society.

[16]  Yan Deng,et al.  Improvement on controllable fabrication of streptavidin-modified three-layer core-shell Fe3O4@SiO2@Au magnetic nanocomposites with low fluorescence background. , 2013, Journal of biomedical nanotechnology.

[17]  Wei Liu,et al.  Metal–organic frameworks for electrochemical applications , 2016 .

[18]  Y. Chai,et al.  Electrochemiluminescent Graphene Quantum Dots as a Sensing Platform: A Dual Amplification for MicroRNA Assay. , 2015, Analytical chemistry.

[19]  Shu-quan Zhang,et al.  Construction of Interpenetrated Ruthenium Metal-Organic Frameworks as Stable Photocatalysts for CO2 Reduction. , 2015, Inorganic chemistry.

[20]  Guonan Chen,et al.  Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification. , 2014, Chemical communications.

[21]  Zong Dai,et al.  A label-free and PCR-free electrochemical assay for multiplexed microRNA profiles by ligase chain reaction coupling with quantum dots barcodes. , 2014, Biosensors & bioelectronics.

[22]  Yan Wang,et al.  A novel bienzyme glucose biosensor based on three-layer Au–Fe3O4@SiO2 magnetic nanocomposite , 2011 .

[23]  I. Imaz,et al.  Nanoscale metal-organic materials. , 2011, Chemical Society reviews.

[24]  Label-free detection of microRNA: two-step signal enhancement with a hairpin-probe-based graphene fluorescence switch and isothermal amplification. , 2013, Chemistry.

[25]  Xiaoyan Wu,et al.  An electrochemical biosensor for sensitive detection of microRNA-155: combining target recycling with cascade catalysis for signal amplification. , 2015, ACS applied materials & interfaces.

[26]  Matt Trau,et al.  Poly(A) Extensions of miRNAs for Amplification-Free Electrochemical Detection on Screen-Printed Gold Electrodes. , 2016, Analytical chemistry.

[27]  Cheng Wang,et al.  Diffusion-controlled luminescence quenching in metal-organic frameworks. , 2011, Journal of the American Chemical Society.

[28]  Y. Chai,et al.  Multiplexed and amplified electronic sensor for the detection of microRNAs from cancer cells. , 2014, Analytical chemistry.

[29]  Jia Guo,et al.  Highly sensitive detection of target ssDNA based on SERS liquid chip using suspended magnetic nanospheres as capturing substrates. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[30]  V. Ambros,et al.  The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 , 1993, Cell.

[31]  W. Miao.  Electrogenerated chemiluminescence and its biorelated applications. , 2008, Chemical reviews.

[32]  Wenbin Lin,et al.  Amplified luminescence quenching of phosphorescent metal-organic frameworks. , 2012, Journal of the American Chemical Society.

[33]  Kai Zhang,et al.  Label-free and ultrasensitive fluorescence detection of cocaine based on a strategy that utilizes DNA-templated silver nanoclusters and the nicking endonuclease-assisted signal amplification method. , 2014, Chemical communications.

[34]  H. Ju,et al.  Highly selective detection of microRNA based on distance-dependent electrochemiluminescence resonance energy transfer between CdTe nanocrystals and Au nanoclusters. , 2014, Biosensors & bioelectronics.

[35]  Demin Liu,et al.  Nanoscale metal-organic frameworks for biomedical imaging and drug delivery. , 2011, Accounts of chemical research.

[36]  Itamar Willner,et al.  Diagnosing the miR-141 prostate cancer biomarker using nucleic acid-functionalized CdSe/ZnS QDs and telomerase† †Electronic supplementary information (ESI) available: Optimization of detection conditions and tabulation of data in Fig. 3. See DOI: 10.1039/c4sc02104e1 Click here for additional data fi , 2014, Chemical science.

[37]  M. Richter.  Electrochemiluminescence (ECL). , 2004, Chemical reviews.

[38]  Z. Gao,et al.  Ligating Dopamine as Signal Trigger onto the Substrate via Metal-Catalyst-Free Click Chemistry for "Signal-On" Photoelectrochemical Sensing of Ultralow MicroRNA Levels. , 2016, Analytical chemistry.

[39]  Zhiqiang Gao,et al.  A label-free biosensor for electrochemical detection of femtomolar microRNAs. , 2013, Analytical chemistry.

[40]  Jae-pyoung Ahn,et al.  Core–Shell Bimetallic Nanoparticles Robustly Fixed on the Outermost Surface of Magnetic Silica Microspheres , 2013, Scientific Reports.

[41]  Yu-Qiang Liu,et al.  One-step, multiplexed fluorescence detection of microRNAs based on duplex-specific nuclease signal amplification. , 2012, Journal of the American Chemical Society.

[42]  Y. Chai,et al.  Signal-off Electrochemiluminescence Biosensor Based on Phi29 DNA Polymerase Mediated Strand Displacement Amplification for MicroRNA Detection. , 2015, Analytical chemistry.

[43]  Qing Hao,et al.  CdS/MoS2 heterojunction-based photoelectrochemical DNA biosensor via enhanced chemiluminescence excitation. , 2016, Biosensors & bioelectronics.

[44]  Paolo Bertoncello,et al.  Nanostructured materials for electrochemiluminescence (ECL)-based detection methods: recent advances and future perspectives. , 2009, Biosensors & bioelectronics.