A Simple High Performance Liquid Chromatography for the Analysis of Cefazolin in Bone

A simple reverse phase – high performance liquid chromatography-photodiode array detection (RP-HPLC-DAD) method for quantitative analysis of cefazolin in human bone was developed. For component separation, the method utilized a C18 column with isocratic elution of an aqueous mobile phase of acetonitrile and 0.1 % (v/v) formic acid (80 : 20) at a flow rate of 1 ml/min. Under the optimal condition, validation of the method demonstrated a good sensitivity, precision and accuracy. The calibration curve was linear for a plot between the peak area ratio of cefazolin and salicylic acid (internal standard), ranging from 0.05-70 mg/L (R 2 > 0.9995). The limits of detection and quantitation were 0.03 and 0.05 mg/L, respectively. The developed method gave satisfactory precision in terms of intraday and interday with RSD of 1.82 and 3.11 (n = 10), respectively. The accuracy of the method was reported as the recovery of nominal spiked amounts versus measured component concentrations. The recoveries were in the range of 97-101 %. The developed method has been applied to determine cefazolin content in cefazolin for injection and the human bone such as tibia and femur.