Serologic and genetic identification of Peromyscus maniculatus as the primary rodent reservoir for a new hantavirus in the southwestern United States.

An outbreak of hantavirus pulmonary syndrome (HPS) in the southwestern United States was etiologically linked to a newly recognized hantavirus. Knowledge that hantaviruses are maintained in rodent reservoirs stimulated a field and laboratory investigation of 1696 small mammals of 31 species. The most commonly captured rodent, the deer mouse (Peromyscus maniculatus), had the highest antibody prevalence (30%) to four hantavirus antigens. Antibody also was detected in 10 other species of rodent and in 1 species of rabbit. Reverse transcriptase-polymerase chain reaction (RT-PCR) products of hantavirus from rodent tissues were indistinguishable from those from human HPS patients. More than 96% of the seropositive P. maniculatus were positive by RT-PCR, suggesting chronic infection. Antibody prevalences were similar among P. maniculatus trapped from Arizona (33%), New Mexico (29%), and Colorado (29%). The numeric dominance of P. maniculatus, the high prevalence of antibody, and the RT-PCR findings implicate this species as the primary rodent reservoir for a new hantavirus in the southwestern United States.

[1]  G. Glass,et al.  Domestic cases of hemorrhagic fever with renal syndrome in the United States. , 1994, Nephron.

[2]  J. Childs,et al.  Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. , 1993, Science.

[3]  S. Nichol,et al.  Rapid detection of vesicular stomatitis virus New Jersey serotype in clinical samples by using polymerase chain reaction , 1993, Journal of clinical microbiology.

[4]  J. Childs,et al.  Hantavirus infection--southwestern United States: interim recommendations for risk reduction. Centers for Disease Control and Prevention. , 1993, MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports.

[5]  Update: outbreak of hantavirus infection--southwestern United States, 1993. , 1993, MMWR. Morbidity and mortality weekly report.

[6]  J. Leduc,et al.  Characterization of Dobrava virus: A hantavirus from Slovenia, Yugoslavia , 1992, Journal of medical virology.

[7]  G. Glass,et al.  Grouping of hantaviruses by small (S) genome segment polymerase chain reaction and amplification of viral RNA from wild-caught rats. , 1992, The American journal of tropical medicine and hygiene.

[8]  S. Xiao,et al.  Belgrade virus: a new hantavirus causing severe hemorrhagic fever with renal syndrome in Yugoslavia. , 1992, The Journal of infectious diseases.

[9]  R. Yanagihara.  Hantavirus infection in the United States: epizootiology and epidemiology. , 1990, Reviews of infectious diseases.

[10]  G. Glass,et al.  Serologic evidence of hantaviral infections within small mammal communities of Baltimore, Maryland: spatial and temporal patterns and host range. , 1989, The American journal of tropical medicine and hygiene.

[11]  J. Dalrymple,et al.  Manual of Hemorrhagic Fever with Renal Syndrome , 1989 .

[12]  M. Miyazaki,et al.  Leakey virus: a new hantavirus isolated from Mus musculus in the United States. , 1988, The Journal of general virology.

[13]  J. Leduc.  Epidemiology of Hantaan and related viruses. , 1987, Laboratory animal science.

[14]  G. Glass,et al.  Epizootiology of Hantavirus infections in Baltimore: isolation of a virus from Norway rats, and characteristics of infected rat populations. , 1987, American journal of epidemiology.

[15]  P. C. Lee,et al.  Serological survey of Prospect Hill virus infection in indigenous wild rodents in the USA. , 1987, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[16]  H. Asada,et al.  Persistent infection of rats with haemorrhagic fever with renal syndrome virus and their antibody responses. , 1986, The Journal of general virology.

[17]  D. Goldgaber,et al.  Partial characterization of Prospect Hill virus isolated from meadow voles in the United States. , 1985, The Journal of infectious diseases.

[18]  D. Gajdusek,et al.  Experimental infection with Puumala virus, the etiologic agent of nephropathia epidemica, in bank voles (Clethrionomys glareolus) , 1985, Journal of virology.

[19]  J. McCormick,et al.  Serological and virological evidence of a Hantaan virus-related enzootic in the United States. , 1985, The Journal of infectious diseases.

[20]  J. Childs,et al.  Geographical distribution and age related prevalence of antibody to Hantaan-like virus in rat populations of Baltimore, Maryland, USA. , 1985, American Journal of Tropical Medicine and Hygiene.

[21]  T. Traavik,et al.  Reservoir animals for nephropathia epidemica in Norway: indications of a major role for the bank vole (C. glareolus) in comparison with the woodmouse (A. sylvaticus) , 1985, Journal of Hygiene.

[22]  J. Leduc,et al.  Hantaan-like viruses from domestic rats captured in the United States. , 1984, The American journal of tropical medicine and hygiene.

[23]  R. Traub,et al.  Prospect Hill virus: serologic evidence for infection in mammologists. , 1984, The New England journal of medicine.

[24]  I. Seong,et al.  Intraspecific transmission of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in the rodent Apodemus agrarius. , 1981, The American journal of tropical medicine and hygiene.