Distribution of GLUT3 glucose transporter protein in human tissues.

To investigate the tissue distribution of the GLUT3 glucose transporter isoform in human tissue we produced affinity purified antibodies to the COOH terminus of the human GLUT3. Both antibodies recognize a specific GLUT3 band in oocytes injected with GLUT3 mRNA but not in those injected with H2O or GLUT1, 2, 4, 5 mRNA. This immunoreactive band in GLUT3 injected oocytes is photolabelled by cytochalasin-B in the presence of L- but not D-glucose indicating that it is a glucose transporter. A high cross reactivity between the human GLUT3 antibodies and a 43 kDa cytoskeletal actin band was identified in all oocyte lysates and many human tissues. However, the specific GLUT3 band could be distinguished from the actin band by carbonate treatment which preferentially solubilized the actin band. Using these antibodies we show that GLUT3 is present as a 45-48 kDa protein in human brain with lower levels detectable in heart, placenta, liver and a barely detectable level in kidney. No GLUT3 was detected in membranes from any of 3 skeletal muscle groups investigated. We conclude that a major role of GLUT3 in humans is as the brain neuronal glucose transporter.

[1]  E. Horton,et al.  Identification of an intracellular pool of glucose transporters from basal and insulin-stimulated rat skeletal muscle. , 1990, The Journal of biological chemistry.

[2]  H. Lodish,et al.  Expression of the low Km GLUT-1 glucose transporter is turned on in perivenous hepatocytes of insulin-deficient diabetic rats. , 1991, Endocrinology.

[3]  H. Imura,et al.  Tissue distribution and species difference of the brain type glucose transporter (GLUT3). , 1991, Biochemical and biophysical research communications.

[4]  C. Burant,et al.  Fructose transporter in human spermatozoa and small intestine is GLUT5. , 1992, The Journal of biological chemistry.

[5]  G I Bell,et al.  Molecular Biology of Mammalian Glucose Transporters , 1990, Diabetes Care.

[6]  J. Lessard,et al.  The nucleotide sequence of a rat vascular smooth muscle α-actin cDNA , 1988 .

[7]  S. Vannucci,et al.  Expression of mouse-GLUT3 and human-GLUT3 glucose transporter proteins in brain. , 1992, Biochemical and biophysical research communications.

[8]  G I Bell,et al.  Evidence for a family of human glucose transporter-like proteins. Sequence and gene localization of a protein expressed in fetal skeletal muscle and other tissues. , 1988, The Journal of biological chemistry.

[9]  G. Bell,et al.  Expression of human glucose transporters in Xenopus oocytes: kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms. , 1991, Biochemistry.

[10]  J. Kornhauser,et al.  Glucose transporter expression in brain. cDNA sequence of mouse GLUT3, the brain facilitative glucose transporter isoform, and identification of sites of expression by in situ hybridization. , 1992, The Journal of biological chemistry.

[11]  B. Kahn.  Alterations in glucose transporter expression and function in diabetes: Mechanisms for insulin resistance , 1992, Journal of cellular biochemistry.

[12]  S. Baldwin,et al.  Anatomical mapping of glucose transporter protein and pyruvate dehydrogenase in rat brain: an immunogold study , 1989, Brain Research.

[13]  M. Weber,et al.  Differential regulation of glucose transporter isoforms by the src oncogene in chicken embryo fibroblasts , 1991, Molecular and cellular biology.

[14]  J. Vandekerckhove,et al.  The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation. , 1979, Differentiation; research in biological diversity.