Excluded Cosolvent in Chromatography.

The concept of cosolvent exclusion was developed by a group of Timasheff's laboratory in 1970-1990 and is currently used widely to explain the effects of a variety of cosolvents on the stability and solubility of macromolecules. Not surprisingly, these concepts have had substantial influence in the fields of formulation, protein folding and unfolding, but they have perhaps more surprisingly found their way into the field of chromatography. A variety of excluded cosolvents have been used to enhance binding and resolution of proteins and other macromolecules in ion exchange, hydroxyapatite, affinity, and hydrophobic interaction chromatography. These cosolvents include salting-out salts, amino acids and polymers, and frequently polyethylene glycol (PEG). A new mode of chromatography, termed "steric exclusion chromatography," was recently introduced. It employs hydroxylated solid phase surfaces. Steric exclusion of the PEG stabilizes the association of macromolecules with the solid phase. Elution is achieved by reducing the PEG concentration. Magnetic particles are also used in this chromatography. This review summarizes the concepts of preferential cosolvent exclusion and its applications in column chromatography.

[1]  P. Gagnon,et al.  Method for obtaining unique selectivities in ion-exchange chromatography by addition of organic polymers to the mobile phase , 1996 .

[2]  J. Lee,et al.  Partial specific volumes and interactions with solvent components of proteins in guanidine hydrochloride. , 1974, Biochemistry.

[3]  Z. Su,et al.  Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography , 1998 .

[4]  J. Lee,et al.  Interaction of calf brain tubulin with poly(ethylene glycols). , 1979, Biochemistry.

[5]  M. Cates,et al.  Depletion force in colloidal systems , 1995 .

[6]  A. Minton,et al.  Influence of macromolecular crowding upon the stability and state of association of proteins: predictions and observations. , 2005, Journal of pharmaceutical sciences.

[7]  S. N. Timasheff,et al.  Interaction of ribonuclease A with aqueous 2-methyl-2,4-pentanediol at pH 5.8. , 1978, Biochemistry.

[8]  S. N. Timasheff,et al.  Preferential binding of solvent components to proteins in mixed water--organic solvent systems. , 1968, Biochemistry.

[9]  D. Ejima,et al.  Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. , 2007, Journal of biochemical and biophysical methods.

[10]  Pete Gagnon,et al.  Improved antibody aggregate removal by hydroxyapatite chromatography in the presence of polyethylene glycol. , 2008, Journal of immunological methods.

[11]  S. N. Timasheff.  Water as ligand: preferential binding and exclusion of denaturants in protein unfolding. , 1992, Biochemistry.

[12]  T. Arakawa,et al.  Mechanism of poly(ethylene glycol) interaction with proteins. , 1985, Biochemistry.

[13]  P. Gagnon,et al.  Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients , 2015, Scientia pharmaceutica.

[14]  P. Gagnon,et al.  High productivity purification of immunoglobulin G monoclonal antibodies on starch-coated magnetic nanoparticles by steric exclusion of polyethylene glycol. , 2014, Journal of chromatography. A.

[15]  D. Ejima,et al.  Solvent modulation of column chromatography. , 2008, Protein and peptide letters.

[16]  D. Bracewell,et al.  A monolith purification process for virus-like particles from yeast homogenate. , 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[17]  M. Popl,et al.  Adsortion elution chromatography of nitrogen-containing heterocycles on alumina , 1972 .

[18]  V. Tuason,et al.  Polyethylene glycol interferes with protein molecular weight determinations by gel filtration. , 1984, Analytical biochemistry.

[19]  S. N. Timasheff,et al.  Preferential interactions of urea with lysozyme and their linkage to protein denaturation. , 2003, Biophysical chemistry.

[20]  T. Arakawa,et al.  The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N'-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. , 1984, The Journal of biological chemistry.

[21]  Marie Tichá,et al.  Some potentialities and drawbacks of contemporary size-exclusion chromatography. , 2003, Journal of biochemical and biophysical methods.

[22]  M. E. Clark,et al.  Living with water stress: evolution of osmolyte systems. , 1982, Science.

[23]  J. F. Wright,et al.  PEG-modulated column chromatography for purification of recombinant adeno-associated virus serotype 9. , 2011, Journal of virological methods.

[24]  T. Arakawa,et al.  Mechanism of protein salting in and salting out by divalent cation salts: balance between hydration and salt binding. , 1984, Biochemistry.

[25]  U. Reichl,et al.  Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol. , 2017, Journal of chromatography. A.

[26]  Fumio Oosawa,et al.  On Interaction between Two Bodies Immersed in a Solution of Macromolecules , 1954 .

[27]  T. Arakawa,et al.  Hydrophobic interaction chromatography in alkaline pH. , 1989, Analytical biochemistry.

[28]  K. Shikama,et al.  Denaturation of Catalase by Freezing and Thawing , 1961, Nature.

[29]  M. Mevarech,et al.  Hydrophobic chromatography and fractionation of enzymes from extremely halophilic bacteria using decreasing concentration gradients of ammonium sulfate. , 1976, Biochemistry.

[30]  J. Lee,et al.  Measurements of preferential solvent interactions by densimetric techniques. , 1979, Methods in enzymology.

[31]  K. D. Collins.  Ion hydration: Implications for cellular function, polyelectrolytes, and protein crystallization. , 2006, Biophysical chemistry.

[32]  S. N. Timasheff,et al.  Protein hydration, thermodynamic binding, and preferential hydration. , 2002, Biochemistry.

[33]  T. Arakawa,et al.  Stabilization of protein structure by sugars. , 1982, Biochemistry.

[34]  S. N. Timasheff,et al.  Preferential and absolute interactions of solvent components with proteins in mixed solvent systems , 1972, Biopolymers.

[35]  T. Arakawa,et al.  Protein stabilization and destabilization by guanidinium salts. , 1984, Biochemistry.

[36]  J. Gibbs.  On the equilibrium of heterogeneous substances , 1878, American Journal of Science and Arts.

[37]  P. Gagnon,et al.  PEG enhances viral clearance on ceramic hydroxyapatite. , 2009, Journal of separation science.

[38]  S. N. Timasheff,et al.  The interaction of beta-lactoglobulin with solvent components in mixed water-organic solvent systems. , 1968, Journal of the American Chemical Society.

[39]  T. Arakawa,et al.  Preferential interactions of proteins with solvent components in aqueous amino acid solutions. , 1983, Archives of biochemistry and biophysics.

[40]  I D Kuntz,et al.  Hydration of Macromolecules , 1969, Science.

[41]  T. Arakawa,et al.  Preferential interactions of proteins with salts in concentrated solutions. , 1982, Biochemistry.

[42]  T. Arakawa,et al.  The stabilization of proteins by osmolytes. , 1985, Biophysical journal.

[43]  Andrés Santos,et al.  The effective colloid interaction in the Asakura-Oosawa model. Assessment of non-pairwise terms from the virial expansion. , 2015, The Journal of chemical physics.

[44]  J. Bi,et al.  Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line. , 2005, Journal of chromatography. A.

[45]  Yan Sun,et al.  Evaluation of steric exclusion chromatography on cryogel column for the separation of serum proteins. , 2014, Journal of chromatography. A.

[46]  J. Traube.  The Attraction Pressure , 1909 .

[47]  A. Podgornik,et al.  Salt tolerant chromatography provides salt tolerance and a better selectivity for protein monomer separations , 2015, Biotechnology journal.

[48]  Solvent modulated linear pH gradient elution for the purification of conventional and bispecific antibodies: Modeling and application. , 2015, Journal of chromatography. A.

[49]  J. Carpenter,et al.  The mechanism of cryoprotection of proteins by solutes. , 1988, Cryobiology.

[50]  Dongxu Zhao,et al.  Polyethylene glycol increases purification and recovery, alters retention behavior in flow-through chromatography of hemoglobin. , 2004, Journal of chromatography. A.

[51]  T. Arakawa,et al.  Interactions of stabilizing additives with proteins during freeze-thawing and freeze-drying. , 1992, Developments in biological standardization.

[52]  U. Reichl,et al.  Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol. , 2017, Journal of chromatography. A.

[53]  D. Winzor,et al.  Effect of sucrose on the dimerization of alpha-chymotrypsin. Allowance for thermodynamic nonideality arising from the presence of a small inert solute. , 1988, Biophysical chemistry.

[54]  S. N. Timasheff.  Protein-solvent interactions and protein conformation , 1970 .

[55]  J. Lee,et al.  The stabilization of proteins by sucrose. , 1981, The Journal of biological chemistry.

[56]  W. Leicht,et al.  Large-scale purification of halophilic enzymes by salting-out mediated chromatography. , 1981, Analytical biochemistry.

[57]  J. Carpenter,et al.  Cryoprotection of phosphofructokinase with organic solutes: characterization of enhanced protection in the presence of divalent cations. , 1986, Archives of biochemistry and biophysics.

[58]  J. Lee,et al.  Preferential solvent interactions between proteins and polyethylene glycols. , 1981, The Journal of biological chemistry.

[59]  S. Neumann,et al.  Mechanism of improved antibody aggregate separation in polyethylene glycol-modulated cation exchange chromatography. , 2012, Journal of separation science.

[60]  S. Ho,et al.  Ion-exchange chromatography of proteins : The effect of neutral polymers in the mobile phase , 1989 .

[61]  T. Arakawa.  The mechanism of increased elution volume of proteins by polyethylene glycol. , 1985, Analytical biochemistry.

[62]  A. Minton,et al.  Quantitative assessment of the relative contributions of steric repulsion and chemical interactions to macromolecular crowding. , 2013, Biopolymers.

[63]  S. N. Timasheff.  Thermodynamic binding and site occupancy in the light of the Schellman exchange concept. , 2002, Biophysical chemistry.

[64]  D. Ejima,et al.  The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals. , 2010, Journal of pharmaceutical sciences.

[65]  S. Sacanna,et al.  Lock and key colloids , 2009, Nature.

[66]  Pete Gagnon,et al.  Principles and applications of steric exclusion chromatography. , 2012, Journal of chromatography. A.

[67]  S. Neumann,et al.  Solvent modulation strategy for superior antibody monomer/aggregate separation in cation exchange chromatography. , 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[68]  Dongxu Zhao,et al.  Purification of Hemoglobin by Ion Exchange Chromatography in Flow-Through Mode with PEG as an Escort , 2004, Artificial cells, blood substitutes, and immobilization biotechnology.

[69]  K. Gekko,et al.  Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures. , 1981, Biochemistry.

[70]  John F. Carpenter,et al.  The basis for toxicity of certain cryoprotectants: A hypothesis , 1990 .