The application of cholinesterase potentiometric biosensor for preliminary screening of the toxicity of waste waters

The application of cholinesterase biosensor for preliminary testing of total pollution of domestic and industrial discharges has been investigated. The results indicate that biosensor-based testing is more sensitive than paramecia bioassay. For numerous samples tested, the inhibition degree of biosensor response exceeds the appropriate value of acute toxicity on Paramecium caudatum. Electrochemical oxidation of the samples, before the biosensor testing readily removes oxidizable compounds which do not effect a real risk of environmental pollution. Simultaneous determination of inhibitory effect, acute toxicity and biochemical (chemical) oxygen demand makes it possible to infer a possible source of contamination of waters.

[1]  A L Ghindilis,et al.  Potentiometric biosensors for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysis. , 1996, Biosensors & bioelectronics.

[2]  Jean-Louis Marty,et al.  Biosensors : potential in pesticide detection , 1995 .

[3]  H. Michel,et al.  Detection and estimation of isopropyl methylphosphonofluoridate and O-ethyl S-diisopropylaminoethylmethylphosphonothioate in sea water in parts-per-trillion level. , 1973, Environmental science & technology.

[4]  Krishna C. Persaud,et al.  Development of an enzyme-based biosensor for atrazine detection , 1993 .

[5]  B. Danielsson,et al.  Trace Level Analysis of Mercury Using Urease in Combination with an Ammonia Gas Sensitive Semiconductor Structure , 1988 .

[6]  Andrea Cagnini,et al.  DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING , 1995 .

[7]  A. Cass,et al.  Inhibited enzyme electrodes. Part 3. A sensor for low levels of H2S and HCN. , 1990, Biosensors & bioelectronics.

[8]  Jean-Louis Marty,et al.  Validation of an enzymatic biosensor with various liquid chromatographic techniques for determining organophosphorus pesticides and carbaryl in freeze-dried waters , 1995 .

[9]  J. Hain,et al.  Catalase biosensor for the determination of hydrogen peroxide, fluoride and cyanide , 1995 .

[10]  H. Budnikov,et al.  Influence of surface-active compounds on the response and sensitivity of cholinesterase biosensors for inhibitor determination. , 1996, The Analyst.

[11]  T. Noguer,et al.  Bi-enzyme amperometric sensor for the detection of ditiocarbamate fungicides , 1993 .

[12]  Kim R. Rogers,et al.  Biosensors for environmental applications , 1995 .

[13]  M. Stoytcheva.  Acetylcholinesterase based amperometric sensor. Inhibitor detection , 1994 .

[14]  Z. Komala.  Paramecium bioassay test in studies on cartap , 1982, Bulletin of environmental contamination and toxicology.

[15]  Gennady Evtugyn,et al.  Electrochemical biosensors for inhibitor determination: Selectivity and sensitivity control , 1996 .

[16]  S. Babkina,et al.  An enzyme amperometric sensor for toxicant determination , 1991 .

[17]  G. Christensen,et al.  Effect of selected water toxicants and other chemicals upon adenosine triphosphatase activityin vitro , 1979, Bulletin of environmental contamination and toxicology.

[18]  G. Christensen,et al.  An enzyme-immobilization procedure for the analysis of enzyme-inhibiting chemicals in water. , 1980, International journal of environmental analytical chemistry.

[19]  Llewellyn R. Williams,et al.  Biosensors for environmental monitoring: a regulatory perspective , 1995 .

[20]  J. Valdés,et al.  Choline derivatives and sodium fluoride protect acetylcholinesterase against irreversible inhibition and aging by DFP and paraoxon. , 1994, Journal of biochemical toxicology.

[21]  A. Amine,et al.  Cyanide Determination Using an Amperometric Biosensor Based on Cytochrome Oxidase Inhibition , 1995 .

[22]  Petr Skládal,et al.  Detection of organophosphate and carbamate pesticides using disposable biosensors based on chemically modified electrodes and immobilized cholinesterase , 1992 .