Sequence of PSE-2 beta-lactamase

The nucleotide sequence of PSE-2 beta-lactamase, an enzyme that readily hydrolyzes both carbenicillin and oxacillin, has been determined. The deduced sequence of 266 amino acids contained 93 residues identical to those of OXA-2 beta-lactamase and the Ser-Thr-Phe-Lys tetrad also found in the active site of TEM-1 beta-lactamase.

[1]  J. Sutcliffe,et al.  Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M. Matthew.  Properties of the β-lactamase specified by the Pseudomonas plasmid R151 , 1978 .

[3]  J. W. Dale,et al.  Sequence of the OXA2 β‐lactamase: comparison with other penicillin‐reactive enzymes , 1985, FEBS letters.

[4]  T. Keys.  Beta-Lactam Antibiotics for Clinical Use , 1987 .

[5]  J. Messing,et al.  Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. , 1983, Gene.

[6]  T. Grundström,et al.  ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Knowles,et al.  Inactivation of the RTEM beta-lactamase from Escherichia coli. Interaction of penam sulfones with enzyme. , 1981, Biochemistry.

[8]  M. Boissinot,et al.  Development of natural and synthetic DNA probes for OXA-2 and TEM-1 beta-lactamases , 1987, Antimicrobial Agents and Chemotherapy.

[9]  J. Richards,et al.  Active-site mutants of beta-lactamase: use of an inactive double mutant to study requirements for catalysis. , 1986, Biochemistry.

[10]  D. Lipman,et al.  Rapid and sensitive protein similarity searches. , 1985, Science.

[11]  G. Jacoby,et al.  Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa , 1983, Antimicrobial Agents and Chemotherapy.

[12]  L. Bryan,et al.  Gentamicin Resistance in Pseudomonas aeruginosa: R-Factor-Mediated Resistance , 1974, Antimicrobial Agents and Chemotherapy.

[13]  M. Matthew.  Plasmid-mediated beta-lactamases of Gram-negative bacteria: properties and distribution. , 1979, The Journal of antimicrobial chemotherapy.

[14]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.