Effects of base-pretreatment on continuous enriched culture for hydrogen production from food waste

Abstract This study aimed to achieve a reliable start-up method for continuous enriched culture for hydrogen production from food waste. When ground and diluted food waste (volatile solids (VS) 4.4 ± 0.2% containing 27 g carbohydrate-chemical oxygen demand/L) was fed, H 2 production decreased below 7.1 mL H 2 /g VS (0.10 mol H 2 /mol hexose added ) within 20 days, because the substrate was consumed via non-H 2 -producing acidogenesis. To suppress the unintended microbial reactions, three methods were examined: lowering H 2 content by continuous CO 2 sparging, acid-pretreatment of food waste at pH 2.0 for 1 day, and base-pretreatment of food waste at pH 12.5 for 1 day. The base-pretreatment reduced indigenous anaerobic bacteria in food waste by 4.9 log and enabled a stable long-term operation over 90 days with the H 2 yields of 62.6 mL H 2 /g VS (0.87 mol H 2 /mol hexose added ). All bacterial species were affiliated with H 2 -producing Clostridium spp. at the cases. Base dosage was increased by 11% compared to the un-pretreated condition.

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