Growing foliose lichens on cover slips: a method for asexual propagation and observing development

Abstract Two temperate foliose lichen species, Parmelia sulcata and Physcia adscendens, were grown outdoors on plastic cover slips to characterize development and study the environmental conditions suitable for development. It was found that aposymbiotic hyphal extensions that adhere to this substratum appeared within two weeks under favorable conditions and usually within five weeks. Pigmentation, rhizines, and epicortical development were seen within six months. The method and month of inoculation onto the cover slips had an impact on developmental success. Our results also suggest suitable combinations of humidity and temperature that may allow routine culture of these lichens in the laboratory setting.

[1]  Y. Gauslaa,et al.  Establishment success of lichen diaspores in young and old boreal rainforests: a comparison between Lobaria pulmonaria and L. scrobiculata , 2011, The Lichenologist.

[2]  T. Bradwell,et al.  Growth of foliose lichens: a review , 2011, Symbiosis.

[3]  S. Joneson,et al.  Fungal and algal gene expression in early developmental stages of lichen-symbiosis , 2011, Mycologia.

[4]  E. Stocker-Wörgötter.  Stress and Developmental Strategies in Lichens , 2010 .

[5]  Y. Kon,et al.  Regeneration of Juvenile Thalli from Transplanted Soredia of Parmotrema clavuliferum and Ramalina yasudae , 2010 .

[6]  S. Joneson,et al.  Compatibility and thigmotropism in the lichen symbiosis: A reappraisal , 2010, Symbiosis.

[7]  M. Sugino,et al.  Cultivation of the lichenParmotrema tinctorum in growth cabinets , 1995, Journal of Plant Research.

[8]  W. B. Sanders,et al.  Observing microscopic phases of lichen life cycles on transparent substrata placed in situ , 2005, The Lichenologist.

[9]  H. Jahns.  Culture experiments with lichens , 1993, Plant Systematics and Evolution.

[10]  R. Türk,et al.  Artificial cultures of the cyanobacterial lichenPeltigera didactyla (Peltigeraceae) in the natural environment , 1989, Plant Systematics and Evolution.

[11]  M. Galun,et al.  A method for the culture of lichen thalli under controlled conditions , 2004, Archiv für Mikrobiologie.

[12]  D. Fahselt,et al.  Cellulose-acetate Disks as Novel Substrate for the Resynthesis and Culture of Lichens , 2003 .

[13]  W. B. Sanders,et al.  In situ development of the foliicolous lichen Phyllophiale (Trichotheliaceae) from propagule germination to propagule production. , 2002, American journal of botany.

[14]  C. Ringli,et al.  Morphological and molecular analysis of early stages in the resynthesis of the lichen Baeomyces rufus , 2002 .

[15]  R. Staples,et al.  Thigmo responses in plants and fungi. , 2002, American journal of botany.

[16]  E. Stocker-Wörgötter.  Experimental Lichenology and Microbiology of Lichens: Culture Experiments, Secondary Chemistry of Cultured Mycobionts, Resynthesis, and Thallus Morphogenesis , 2001 .

[17]  T. Green,et al.  Hydration-dependent photosynthetic production of lichens: what do laboratory studies tell us about field performance? , 2001, Journal of experimental botany.

[18]  I. Brodo,et al.  Lichens of North America , 2002 .

[19]  S. Zoller,et al.  Juvenile Development and Diaspore Survival in the Threatened Epiphytic Lichen Species Sticta fuliginosa, Leptogium saturninum and Menegazzia terebrata: Conclusions for in situ Conservation , 2000 .

[20]  R. Honegger.  Experimental studies with foliose macrolichens: fungal responses to spatial disturbance at the organismic level and to spatial problems at the cellular level during drought stress events I , 1995 .

[21]  E. Stocker-Wörgötter.  Experimental cultivation of lichens and lichen symbionts , 1995 .

[22]  C. Scheidegger.  Early Development of Transplanted Isidioid Soredia of Lobaria Pulmonaria in an Endangered Population , 1995, The Lichenologist.

[23]  R. Honegger.  Developmental biology of lichens. , 1993, The New phytologist.

[24]  R. Honegger.  A simple outdoor culturing system for the foliose macrolichens Xanthoria parietina (L.) Th. Fr. and Parmelia sulcata Taylor , 1993 .

[25]  D. Armaleo.  Experimental Microbiology of Lichens: Mycelia Fragmentation, A Novel Growth Chamber, and the Origins of Thallus Differentiation , 1991 .

[26]  H. Jahns.  The establishment, individuality and growth of lichen thalli , 1988 .

[27]  S. Ott.  Differences in the developmental rates of lichens , 1987 .

[28]  S. Ott.  The Juvenile Development of Lichen Thalli from Vegetative Diaspores , 1987 .

[29]  P. Modenesi,et al.  The Cortical Surfaces in Parmelia Saxatilis and P.Caperata: A Histochemical Approach , 1986, The Lichenologist.

[30]  S. Ott,et al.  Artificial Cultures of Lichens in the Natural Environment , 1985, The Lichenologist.

[31]  C. Veltkamp,et al.  The Upper Cortex of Parmelia Saxatilis and other Lichen Thalli , 1982, The Lichenologist.

[32]  M. E. Hale.  Fine structure of the cortex in the lichen family Parmeliaceae viewed with the scanning-electron microscope , 1973 .

[33]  L. Pearson.  VARYING ENVIRONMENTAL FACTORS IN ORDER TO GROW INTACT LICHENS UNDER LABORATORY CONDITIONS , 1970 .

[34]  Harold C. Bold,et al.  The Morphology of Chlamydomonas chlamydogama, Sp. Nov. , 1949 .

[35]  K. Goebel.  Morphologische und biologische Bemerkungen: 32. Induzierte Dorsiventralität bei Flechten. , 1926 .