Insulin‐like growth factor‐1 receptors and insulin‐like growth factor‐1‐like activity in human primary breast cancer

In the current study the authors have investigated whether human primary breast cancer specimens contain insulin‐like growth factor‐1 (IGF‐1) receptors (IGF‐1‐R) or IGF‐1‐like activities. Simultaneously, epidermal growth factor (EGF) receptors (EGF‐R) and cytosolic estrogen receptor (ER), progesterone receptor (PR), and EGF‐like activity were determined. All tumors assayed contained a single class of specific iodine 125 (125I)‐IGF‐1 binding sites (Kd: median 106, range 48–755 pM; n = 32) with limited capacity (Bmax: median 147, range 19–11,900 fmol/mg membrane protein). Seventy percent of 44 tumors (50% ER+, PR+), displayed specific 125I‐EGF binding with a wide range of values (median 13, range 2–215 fmol/mg protein, n = 31). A positive relationship was apparent between the amount of IGF‐1‐R with ER and PR (Spearman; 2P < 0.02 and 2P < 0.01, respectively; n = 32), whereas for EGF‐R a negative relationship was observed (for both 2P < 0.01; n = 44). All tumors contained endogeneous IGF‐1‐like and EGF‐like activities as measured by radioreceptor assay on acid‐ethanol extracted cytosols (median 15, range 3–131 ng/mg protein, n = 78 for IGF‐1; and median 86, range 26–517 ng/mg protein, n = 142 for EGF). Tumor contents of IGF‐1‐like and EGF‐like activities showed a negative relationship with ER (2P < 0.1 for IGF‐1, n = 78; and 2P < 0.001 for EGF, n = 142), and with PR (2P < 0.05, n = 78; and 2P < 0.001, n = 142). No relationship was observed between the tumor contents of IGF‐1‐like and EGF‐like activities. In conclusion, these data support the view that IGF‐1 and EGF can act as autocrine or paracrine growth factors in human breast cancer.

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