Single Molecule Spectroscopy of the Green Fluorescent Protein: A Critical Assessment

[1]  Toshio Yanagida,et al.  Single-Molecule Imaging of Signaling Molecules in Living Cells , 2000 .

[2]  Jerker Widengren,et al.  Protonation kinetics of GFP and FITC investigated by FCS — aspects of the use of fluorescent indicators for measuring pH , 1999 .

[3]  M. A. Bopp,et al.  Fluorescence and photobleaching dynamics of single light-harvesting complexes. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[4]  E. Peterman,et al.  Optical methods for exploring dynamics of single copies of green fluorescent protein. , 1999, Cytometry.

[5]  W. E. Moerner,et al.  Single-Molecule Fluorescence Resonant Energy Transfer in Calcium Concentration Dependent Cameleon , 2000 .

[6]  Ronald D. Vale,et al.  Imaging individual green fluorescent proteins , 1997, Nature.

[7]  W E Moerner,et al.  Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Werner Baumgartner,et al.  Characterization of Photophysics and Mobility of Single Molecules in a Fluid Lipid Membrane , 1995 .

[9]  Douglas C. Youvan,et al.  Time-resolved spectroscopy of wild-type and mutant Green Fluorescent Proteins reveals excited state deprotonation consistent with fluorophore-protein interactions , 1996 .

[10]  S. Weiss Fluorescence spectroscopy of single biomolecules. , 1999, Science.

[11]  R. Rigler,et al.  Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study , 1995 .

[12]  J. Haseloff,et al.  Molecular Characterization of Recombinant Green Fluorescent Protein by Fluorescence Correlation Microscopy , 1995 .

[13]  B. Steipe,et al.  Confocal microscopy of single molecules of the green fluorescent protein , 1998 .

[14]  W. M. Westler,et al.  Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. , 1993, Biochemistry.

[15]  S J Remington,et al.  Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. Verkman,et al.  Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. , 1997, Biophysical journal.

[17]  X. Xie,et al.  Optical studies of single molecules at room temperature. , 1998, Annual review of physical chemistry.

[18]  W. Webb,et al.  Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. , 1999, Biophysical journal.

[19]  A S Verkman,et al.  Green fluorescent protein as a noninvasive intracellular pH indicator. , 1998, Biophysical journal.

[20]  V. Subramaniam,et al.  Photophysics and optical switching in green fluorescent protein mutants. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Gerald Kada,et al.  Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy , 2000, The EMBO journal.

[22]  Ronald D Vale,et al.  The Directional Preference of Kinesin Motors Is Specified by an Element outside of the Motor Catalytic Domain , 1997, Cell.

[23]  Notker Rösch,et al.  Structure and rotation barriers for ground and excited states of the isolated chromophore of the green fluorescent protein , 1998 .

[24]  G. Phillips,et al.  The molecular structure of green fluorescent protein , 1996, Nature Biotechnology.

[25]  R. Tsien,et al.  Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer , 1996, Current Biology.

[26]  T. Kues,et al.  Imaging and tracking of single GFP molecules in solution. , 2000, Biophysical journal.

[27]  B G de Grooth,et al.  Visualising individual green fluorescent proteins with a near field optical microscope. , 1999, Cytometry.

[28]  R. Vale,et al.  Assaying processive movement of kinesin by fluorescence microscopy. , 1998, Methods in enzymology.

[29]  R. Vale,et al.  Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics. , 1999 .

[30]  B. Steipe,et al.  Stable Low-Temperature Photoproducts and Hole Burning of Green Fluorescent Protein (GFP) , 1999 .

[31]  Toshio Yanagida,et al.  Single-molecule imaging of EGFR signalling on the surface of living cells , 2000, Nature Cell Biology.

[32]  A. Oijen,et al.  Unraveling the electronic structure of individual photosynthetic pigment-protein complexes , 1999, Science.

[33]  C. Scharnagl,et al.  Molecular basis for pH sensitivity and proton transfer in green fluorescent protein: protonation and conformational substates from electrostatic calculations. , 1999, Biophysical journal.

[34]  R. Tsien,et al.  On/off blinking and switching behaviour of single molecules of green fluorescent protein , 1997, Nature.

[35]  W. E. Moerner,et al.  The Fluorescence Dynamics of Single Molecules of Green Fluorescent Protein , 1999 .

[36]  X. Xie,et al.  Single-molecule enzymatic dynamics. , 1998, Science.

[37]  M. Eigen,et al.  Sorting single molecules: application to diagnostics and evolutionary biotechnology. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[38]  A Wlodawer,et al.  Spectral variants of green fluorescent protein. , 1999, Methods in enzymology.

[39]  Notker Rösch,et al.  Quantum chemical modeling of structure and absorption spectra of the chromophore in green fluorescent proteins , 1998 .

[40]  Notker Rösch,et al.  PROTONATION EFFECTS ON THE CHROMOPHORE OF GREEN FLUORESCENT PROTEIN. QUANTUM CHEMICAL STUDY OF THE ABSORPTION SPECTRUM , 1997 .

[41]  R. Tsien,et al.  green fluorescent protein , 2020, Catalysis from A to Z.

[42]  Gregor Jung,et al.  The Role of Dark States in the Photodynamics of the Green Fluorescent Protein Examined with Two-Color Fluorescence Excitation Spectroscopy , 2000 .

[43]  V. Subramaniam,et al.  Three photoconvertible forms of green fluorescent protein identified by spectral hole-burning , 1999, Nature Structural Biology.

[44]  W. Webb,et al.  Fluorescence correlation spectroscopy: diagnostics for sparse molecules. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[45]  M. Giacca,et al.  The Enhanced Green Fluorescent Protein as a Tool for the Analysis of Protein Dynamics and Localization: Local Fluorescence Study at the Single‐molecule Level , 2000, Photochemistry and photobiology.

[46]  S. Boxer,et al.  Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[47]  C. Bräuchle,et al.  Direct spectroscopic observation of quantum jumps of a single molecule , 1995, Nature.

[48]  J. Schlichter,et al.  Protein dynamics at low temperatures , 2000 .

[49]  M. Orrit,et al.  Single pentacene molecules detected by fluorescence excitation in a p-terphenyl crystal. , 1990, Physical review letters.

[50]  W. Webb,et al.  Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[51]  O. Ohara,et al.  Single molecular assay of individual ATP turnover by a myosin‐GFP fusion protein expressed in vitro , 1997, FEBS letters.

[52]  Jerker Widengren,et al.  Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy , 1999 .

[53]  S. Lukyanov,et al.  Fluorescent proteins from nonbioluminescent Anthozoa species , 1999, Nature Biotechnology.

[54]  Roger Y. Tsien,et al.  Crystal Structure of the Aequorea victoria Green Fluorescent Protein , 1996, Science.

[55]  T. Straatsma,et al.  Internal Dynamics of Green Fluorescent Protein , 1999 .

[56]  J Greve,et al.  Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules. , 2000, Proceedings of the National Academy of Sciences of the United States of America.