Preparation of a biphasic scaffold for osteochondral tissue engineering

Abstract Tissue engineering has been developed as a prospective approach for the repair of articular cartilage defects. Engineered osteochondral implants can facilitate the fixation and integration with host tissue, and therefore promote the regeneration of osteochondral defects. A biphasic scaffold with a stratified two-layer structure for osteochondral tissue engineering was developed from biodegradable synthetic and naturally derived polymers. The upper layer of the scaffold for cartilage engineering was collagen sponge; the lower layer for bone engineering was a composite sponge of poly(DL-lactic-co-glycolic acid) (PLGA) and naturally derived collagen. The PLGA–collagen composite sponge layer had a composite structure with collagen microsponge formed in the pores of a skeleton PLGA sponge. The collagen sponge in the two respective layers was connected. Observation of the collagen/PLGA–collagen biphasic scaffold by scanning electron microscopy (SEM) demonstrated the connected stratified structure. The biphasic scaffold was used for culture of canine bone-marrow-derived mesenchymal stem cells. The cell/scaffold construct was implanted in an osteochondral defect in the knee of a one-year old beagle. Osteochondral tissue was regenerated four months after implantation. Cartilage- and bone-like tissues were formed in the respective layers. The collagen/PLGA–collagen biphasic scaffold will be useful for osteochondral tissue engineering.

[1]  Hwa-Chang Liu,et al.  Cartilage tissue engineering on the surface of a novel gelatin-calcium-phosphate biphasic scaffold in a double-chamber bioreactor. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[2]  Guoping Chen,et al.  The use of a novel PLGA fiber/collagen composite web as a scaffold for engineering of articular cartilage tissue with adjustable thickness. , 2003, Journal of biomedical materials research. Part A.

[3]  Guoping Chen,et al.  Redifferentiation of dedifferentiated bovine chondrocytes when cultured in vitro in a PLGA–collagen hybrid mesh , 2003, FEBS letters.

[4]  M Sittinger,et al.  Tissue engineering of biphasic joint cartilage transplants. , 1999, Biomaterials.

[5]  Guoping Chen,et al.  Evaluation of PLLA-collagen hybrid sponge as a scaffold for cartilage tissue engineering , 2004 .

[6]  Junzo Tanaka,et al.  Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold. , 2004, Biochemical and biophysical research communications.

[7]  Guoping Chen,et al.  Tissue engineering of cartilage using a hybrid scaffold of synthetic polymer and collagen. , 2004, Tissue engineering.

[8]  Takashi Ushida,et al.  Collagen hybridization with poly(l-lactic acid) braid promotes ligament cell migration , 2001 .

[9]  R Kujat,et al.  Engineering of osteochondral tissue with bone marrow mesenchymal progenitor cells in a derivatized hyaluronan-gelatin composite sponge. , 1999, Tissue engineering.

[10]  Mark A. Randolph,et al.  Tissue Engineered Neocartilage Using Plasma Derived Polymer Substrates and Chondrocytes , 1998, Plastic and reconstructive surgery.

[11]  Guoping Chen,et al.  Application of PLGA-collagen hybrid mesh for three-dimensional culture of canine anterior cruciate ligament cells , 2004 .

[12]  R Langer,et al.  Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. , 1993, Journal of biomedical materials research.

[13]  R Langer,et al.  Cell seeding in porous transplantation devices. , 1993, Biomaterials.

[14]  Alan Grodzinsky,et al.  Tissue-engineered composites for the repair of large osteochondral defects. , 2002, Arthritis and rheumatism.

[15]  K. Fujii,et al.  Use of a biphasic graft constructed with chondrocytes overlying a beta-tricalcium phosphate block in the treatment of rabbit osteochondral defects. , 2005, Tissue engineering.

[16]  Véronique Maquet,et al.  Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study. , 2004, Biomaterials.

[17]  Guoping Chen,et al.  Regeneration of cartilage tissue by combination of canine chondrocyte and a hybrid mesh scaffold , 2004 .

[18]  R Langer,et al.  In vitro generation of osteochondral composites. , 2000, Biomaterials.

[19]  Scott C. Brown,et al.  A three-dimensional osteochondral composite scaffold for articular cartilage repair. , 2002, Biomaterials.

[20]  Scott J Hollister,et al.  Engineered osteochondral grafts using biphasic composite solid free-form fabricated scaffolds. , 2004, Tissue engineering.

[21]  H Planck,et al.  Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage. , 1998, Journal of biomedical materials research.

[22]  A I Caplan,et al.  Tissue-engineered fabrication of an osteochondral composite graft using rat bone marrow-derived mesenchymal stem cells. , 2001, Tissue engineering.

[23]  Takashi Ushida,et al.  Development of biodegradable porous scaffolds for tissue engineering , 2001 .

[24]  C. Archer,et al.  Current strategies for articular cartilage repair. , 2005, European cells & materials.

[25]  J. Mason,et al.  Cartilage tissue engineering: current limitations and solutions. , 1999, Clinical orthopaedics and related research.

[26]  L. Hangody,et al.  Arthroscopic autogenous osteochondral mosaicplasty for the treatment of femoral condylar articular defects A preliminary report , 1997, Knee Surgery, Sports Traumatology, Arthroscopy.

[27]  Guoping Chen,et al.  Scaffold Design for Tissue Engineering , 2002 .

[28]  Jeremy J Mao,et al.  Tissue-engineered osteochondral constructs in the shape of an articular condyle. , 2005, The Journal of bone and joint surgery. American volume.