A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins

[1]  Hui Zheng,et al.  Practical considerations for choosing a mouse model of Alzheimer’s disease , 2017, Molecular Neurodegeneration.

[2]  P. Hunter.  The reproducibility “crisis” , 2017, EMBO reports.

[3]  B. Winblad,et al.  APP mouse models for Alzheimer's disease preclinical studies , 2017, The EMBO journal.

[4]  F. Korner‐Nievergelt,et al.  The earth is flat (p > 0.05): significance thresholds and the crisis of unreplicable research , 2017, PeerJ.

[5]  A. Buisson,et al.  The amyloid-β oligomer Aβ*56 induces specific alterations in neuronal signaling that lead to tau phosphorylation and aggregation , 2017, Science Signaling.

[6]  D. Drucker,et al.  Never Waste a Good Crisis: Confronting Reproducibility in Translational Research. , 2016, Cell metabolism.

[7]  Emma Lundberg,et al.  A proposal for validation of antibodies , 2016, Nature Methods.

[8]  Thomas C. Südhof,et al.  Truth in Science Publishing: A Personal Perspective , 2016, PLoS biology.

[9]  M. Baker.  1,500 scientists lift the lid on reproducibility , 2016, Nature.

[10]  M. Frosch,et al.  The aqueous phase of Alzheimer's disease brain contains assemblies built from ∼4 and ∼7 kDa Aβ species , 2015, Alzheimer's & Dementia.

[11]  M. Baker.  Reproducibility crisis: Blame it on the antibodies , 2015, Nature.

[12]  Andreas Plückthun,et al.  Reproducibility: Standardize antibodies used in research , 2015, Nature.

[13]  J. Ioannidis,et al.  Reproducibility in Science: Improving the Standard for Basic and Preclinical Research , 2015, Circulation research.

[14]  C. Begley,et al.  Drug development: Raise standards for preclinical cancer research , 2012, Nature.

[15]  F. Prinz,et al.  Believe it or not: how much can we rely on published data on potential drug targets? , 2011, Nature Reviews Drug Discovery.

[16]  R. S. Stainton,et al.  OR—a personal perspective , 2010, J. Oper. Res. Soc..

[17]  R. Gravel,et al.  Paradoxical Regulation of Biotin Utilization in Brain and Liver and Implications for Inherited Multiple Carboxylase Deficiency* , 2004, Journal of Biological Chemistry.

[18]  Carl W. Cotman,et al.  Common Structure of Soluble Amyloid Oligomers Implies Common Mechanism of Pathogenesis , 2003, Science.

[19]  P. Selby,et al.  Use of a sensitive EnVision™+‐based detection system for Western blotting: avoidance of streptavidin binding to endogenous biotin and biotin‐containing proteins in kidney and other tissues , 2003, Proteomics.

[20]  Kang Hu,et al.  High-Level Neuronal Expression of Aβ1–42 in Wild-Type Human Amyloid Protein Precursor Transgenic Mice: Synaptotoxicity without Plaque Formation , 2000, The Journal of Neuroscience.

[21]  J. Pevsner,et al.  Detection of endogenous biotin in various tissues: novel functions in the hippocampus and implications for its use in avidin-biotin technology , 1999, Cell and Tissue Research.

[22]  R. Sanderson,et al.  Modification of enzyme-conjugated streptavidin-biotin western blot technique to avoid detection of endogenous biotin-containing proteins. , 1999, BioTechniques.

[23]  C. Praul,et al.  Detection of endogenous biotin-containing proteins in bone and cartilage cells with streptavidin systems. , 1998, Biochemical and biophysical research communications.

[24]  S. Younkin,et al.  Correlative Memory Deficits, Aβ Elevation, and Amyloid Plaques in Transgenic Mice , 1996, Science.

[25]  D. Borchelt,et al.  Age-related CNS disorder and early death in transgenic FVB/N mice overexpressing Alzheimer amyloid precursor proteins , 1995, Neuron.

[26]  N. T. Davis,et al.  Biotin-containing proteins of the insect nervous system, a potential source of interference with immunocytochemical localization procedures. , 1995, Insect biochemistry and molecular biology.

[27]  O. Mäkelä,et al.  The Alternative Binding Site for Protein A in the Fab Fragment of Immunoglobulins , 1993, Scandinavian journal of immunology.

[28]  T. Haneji,et al.  Transblot identification of biotin-containing proteins in rat liver. , 1989, Analytical biochemistry.

[29]  L. Björck,et al.  Streptococcal protein G has affinity for both Fab- and Fc-fragments of human IgG. , 1988, Molecular immunology.

[30]  D. Wolock,et al.  Staphylococcal protein A binding to the Fab fragments of mouse monoclonal antibodies. , 1984, Journal of immunology.

[31]  S. Lesné,et al.  Detecting aβ*56 oligomers in brain tissues. , 2011, Methods in molecular biology.