Metagenome-Assembled Genome of a Cyclobacteriaceae Bacterium, HetDA_MAG_MS6, Isolated from a Trichodesmium Consortium from Station ALOHA

Here, we describe the metagenome-assembled genome (MAG) HetDA_MAG_MS6. HetDA_MAG_MS6 was obtained from an enrichment of the heterocystous diazotroph HetDA, which was isolated near Station ALOHA. The MAG was placed in the Cyclobacteriaceae family and is predicted to be a chemoorganoheterotroph with the potential for ammonia uptake, phosphonate transport, and sulfolipid biosynthesis. ABSTRACT Here, we describe the metagenome-assembled genome (MAG) HetDA_MAG_MS6. HetDA_MAG_MS6 was obtained from an enrichment of the heterocystous diazotroph HetDA, which was isolated near Station ALOHA. The MAG was placed in the Cyclobacteriaceae family and is predicted to be a chemoorganoheterotroph with the potential for ammonia uptake, phosphonate transport, and sulfolipid biosynthesis.

[1]  Christopher J Neely,et al.  MetaSanity: an integrated microbial genome evaluation and annotation pipeline , 2020, Bioinform..

[2]  Donovan H Parks,et al.  GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database , 2019, Bioinform..

[3]  Feng Li,et al.  MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies , 2019, PeerJ.

[4]  Donovan H. Parks,et al.  A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life , 2018, Nature Biotechnology.

[5]  P. Pevzner,et al.  metaSPAdes: a new versatile metagenomic assembler. , 2017, Genome research.

[6]  Alexander J Probst,et al.  Recovery of genomes from metagenomes via a dereplication, aggregation and scoring strategy , 2017, Nature Microbiology.

[7]  L. Hudek,et al.  Role of Phosphate Transport System Component PstB1 in Phosphate Internalization by Nostoc punctiforme , 2016, Applied and Environmental Microbiology.

[8]  Elaina D. Graham,et al.  BinSanity: unsupervised clustering of environmental microbial assemblies using coverage and affinity propagation , 2016, bioRxiv.

[9]  J. Brandsma,et al.  Lipid remodelling is a widespread strategy in marine heterotrophic bacteria upon phosphorus deficiency , 2015, The ISME Journal.

[10]  Connor T. Skennerton,et al.  CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes , 2015, Genome research.

[11]  Patrick K. H. Lee,et al.  A novel cohabitation between two diazotrophic cyanobacteria in the oligotrophic ocean , 2014, The ISME Journal.

[12]  Anders F. Andersson,et al.  Binning metagenomic contigs by coverage and composition , 2014, Nature Methods.

[13]  Björn Usadel,et al.  Trimmomatic: a flexible trimmer for Illumina sequence data , 2014, Bioinform..

[14]  J. Montoya,et al.  Nitrogenase (nifH) gene expression in diazotrophic cyanobacteria in the Tropical North Atlantic in response to nutrient amendments , 2012, Front. Microbio..

[15]  E. Carpenter,et al.  Trichodesmium – a widespread marine cyanobacterium with unusual nitrogen fixation properties , 2012, FEMS microbiology reviews.

[16]  Steven L Salzberg,et al.  Fast gapped-read alignment with Bowtie 2 , 2012, Nature Methods.

[17]  J. McGrath,et al.  The Genes and Enzymes of Phosphonate Metabolism by Bacteria, and Their Distribution in the Marine Environment , 2011, Front. Microbio..

[18]  Gabrielle Rocap,et al.  Sulfolipids dramatically decrease phosphorus demand by picocyanobacteria in oligotrophic marine environments. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[19]  J. Zehr,et al.  GROWTH AND NITROGEN FIXATION OF THE DIAZOTROPHIC FILAMENTOUS NONHETEROCYSTOUS CYANOBACTERIUM TRICHODESMIUM SP. IMS 101 IN DEFINED MEDIA: EVIDENCE FOR A CIRCADIAN RHYTHM 1 , 1996 .