Site-directed mutagenesis of aspartate aminotransferase from E. coli.

[1]  H. Kagamiyama,et al.  Aspartate aminotransferase of Escherichia coli: nucleotide sequence of the aspC gene. , 1985, Journal of biochemistry.

[2]  A. Fersht,et al.  Hydrogen bonding and biological specificity analysed by protein engineering , 1985, Nature.

[3]  H. K. Schachman,et al.  Site-specific mutagenesis of aspartate transcarbamoylase. Replacement of tyrosine 165 in the catalytic chain by serine reduces enzymatic activity. , 1984, The Journal of biological chemistry.

[4]  H. Kagamiyama,et al.  The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes. , 1984, Biochemical and biophysical research communications.

[5]  G. Eichele,et al.  Mechanism of action of aspartate aminotransferase proposed on the basis of its spatial structure. , 1984, Journal of molecular biology.

[6]  G. Petsko,et al.  Probing the catalytic mechanism of yeast triose phosphate isomerase by site-specific mutagenesis. , 1984, Biochemical Society transactions.

[7]  J. Kraut,et al.  Directed mutagenesis of dihydrofolate reductase. , 1983, Science.

[8]  Keith C. Norris,et al.  Efficient site-directed mutagenesis by simultaneous use of two primers. , 1983, Nucleic acids research.

[9]  D. Hanahan.  Studies on transformation of Escherichia coli with plasmids. , 1983, Journal of molecular biology.

[10]  K. Inokuchi,et al.  Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. , 1982, Nucleic acids research.

[11]  J. Vieira,et al.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. , 1982, Gene.

[12]  J. Vieira,et al.  A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. , 1982, Gene.

[13]  D. Holmes,et al.  A rapid boiling method for the preparation of bacterial plasmids. , 1981, Analytical biochemistry.

[14]  J. Sadler,et al.  Plasmids containing many tandem copies of a synthetic lactose operator. , 1980, Gene.

[15]  H. Kagamiyama,et al.  A sensitive method for the detection of aspartate: 2-oxoglutarate aminotransferase activity of polyacrylamide gels. , 1981, Analytical biochemistry.