Colorimetric Detection of Escherichia coli Based on the Enzyme-Induced Metallization of Gold Nanorods.

A novel enzyme-induced metallization colorimetric assay is developed to monitor and measure beta-galactosidase (β-gal) activity, and is further employed for colorimetric bacteriophage (phage)-enabled detection of Escherichia coli (E. coli). This assay relies on enzymatic reaction-induced silver deposition on the surface of gold nanorods (AuNRs). In the presence of β-gal, the substrate p-aminophenyl β-d-galactopyranoside is hydrolyzed to produce p-aminophenol (PAP). Reduction of silver ions by PAP generates a silver shell on the surface of AuNRs, resulting in the blue shift of the longitudinal localized surface plasmon resonance peak and multicolor changes of the detection solution from light green to orange-red. Under optimized conditions, the detection limit for β-gal is 128 pM, which is lower than the conventional colorimetric assay. Additionally, the assay has a broader dynamic range for β-gal detection. The specificity of this assay for the detection of β-gal is demonstrated against several protein competitors. Additionally, this technique is successfully applied to detect E. coli bacteria cells in combination with bacteriophage infection. Due to the simplicity and short incubation time of this enzyme-induced metallization colorimetric method, the assay is well suited for the detection of bacteria in low-resource settings.

[1]  G. Whitesides,et al.  Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. , 2008, Analytical chemistry.

[2]  Vincent M Rotello,et al.  UV-nanoimprint lithography as a tool to develop flexible microfluidic devices for electrochemical detection. , 2015, Lab on a chip.

[3]  Catherine J. Murphy,et al.  Seed-Mediated Synthesis of Gold Nanorods: Role of the Size and Nature of the Seed , 2004 .

[4]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[5]  P A Singer,et al.  Grand Challenges in Global Health , 2003, Science.

[6]  Chad A Mirkin,et al.  Capillary force-driven, large-area alignment of multi-segmented nanowires. , 2014, ACS nano.

[7]  X. Qu,et al.  Colorimetric Biosensing Using Smart Materials , 2011, Advanced materials.

[8]  Catherine J. Murphy,et al.  Wet Chemical Synthesis of High Aspect Ratio Cylindrical Gold Nanorods , 2001 .

[9]  Zoraida P. Aguilar,et al.  Amperometric determination of live Escherichia coli using antibody-coated paramagnetic beads , 2005, Analytical and bioanalytical chemistry.

[10]  Sindy K. Y. Tang,et al.  Filter-based assay for Escherichia coli in aqueous samples using bacteriophage-based amplification. , 2013, Analytical chemistry.

[11]  S. Hou,et al.  Electrochemical nanoparticle-enzyme sensors for screening bacterial contamination in drinking water. , 2015, The Analyst.

[12]  Zhen Fan,et al.  Nanomaterials for targeted detection and photothermal killing of bacteria. , 2012, Chemical Society reviews.

[13]  J Rishpon,et al.  Combined phage typing and amperometric detection of released enzymatic activity for the specific identification and quantification of bacteria. , 2003, Analytical chemistry.

[14]  Xiaomei Yan,et al.  Trace detection of specific viable bacteria using tetracysteine-tagged bacteriophages. , 2014, Analytical chemistry.

[15]  Sarit S. Agasti,et al.  Gold nanoparticles in chemical and biological sensing. , 2012, Chemical reviews.

[16]  Pierre Servais,et al.  Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. , 2002, Journal of microbiological methods.

[17]  Catherine J. Murphy,et al.  An Improved Synthesis of High‐Aspect‐Ratio Gold Nanorods , 2003 .

[18]  Wei Qian,et al.  Cancer cells assemble and align gold nanorods conjugated to antibodies to produce highly enhanced, sharp, and polarized surface Raman spectra: a potential cancer diagnostic marker. , 2007, Nano letters.

[19]  H. Anany,et al.  Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout , 2014, Analytical and Bioanalytical Chemistry.

[20]  M. El-Sayed,et al.  Simulation of the Optical Absorption Spectra of Gold Nanorods as a Function of Their Aspect Ratio and the Effect of the Medium Dielectric Constant , 1999 .

[21]  Ratmir Derda,et al.  Portable self-contained cultures for phage and bacteria made of paper and tape. , 2012, Lab on a chip.

[22]  Chao Zhang,et al.  Time--temperature indicator for perishable products based on kinetically programmable Ag overgrowth on Au nanorods. , 2013, ACS nano.

[23]  Joseph Irudayaraj,et al.  Gold nanorod probes for the detection of multiple pathogens. , 2008, Small.

[24]  P. V. van Helden,et al.  Phage-based detection of bacterial pathogens. , 2014, The Analyst.

[25]  Vincent M Rotello,et al.  Detection of Escherichia coli in drinking water using T7 bacteriophage-conjugated magnetic probe. , 2015, Analytical chemistry.

[26]  Vincent M. Rotello,et al.  Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor. , 2011, Journal of the American Chemical Society.

[27]  Tianran Lin,et al.  Visual and colorimetric detection of p-aminophenol in environmental water and human urine samples based on anisotropic growth of Ag nanoshells on Au nanorods. , 2016, Talanta.

[28]  Zhiqiang Gao,et al.  Enzyme-catalysed deposition of ultrathin silver shells on gold nanorods: a universal and highly efficient signal amplification strategy for translating immunoassay into a litmus-type test. , 2015, Chemical communications.

[29]  Kate E. Jones,et al.  Global trends in emerging infectious diseases , 2008, Nature.

[30]  Bradley Duncan,et al.  Bacteriophage-based nanoprobes for rapid bacteria separation. , 2015, Nanoscale.

[31]  A. Jayaraman,et al.  Preventing adhesion of Escherichia coli O157:H7 and Salmonella Typhimurium LT2 on tomato surfaces via ultrathin polyethylene glycol film. , 2014, International journal of food microbiology.

[32]  Paul Yager,et al.  Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection. , 2012, Analytical chemistry.

[33]  Sam R. Nugen,et al.  Rapid detection of Salmonella using a redox cycling-based electrochemical method , 2016 .

[34]  Manuel Miró,et al.  High-resolution colorimetric assay for rapid visual readout of phosphatase activity based on gold/silver core/shell nanorod. , 2014, ACS applied materials & interfaces.

[35]  Chad A Mirkin,et al.  Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties. , 2007, Nano letters.

[36]  Hongje Jang,et al.  Graphene oxide for fluorescence-mediated enzymatic activity assays. , 2014, Journal of materials chemistry. B.

[37]  Eva Baldrich,et al.  Amperometric detection of Enterobacteriaceae in river water by measuring β-galactosidase activity at interdigitated microelectrode arrays. , 2010, Analytica chimica acta.

[38]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[39]  B. Nikoobakht,et al.  種結晶を媒介とした成長法を用いた金ナノロッド(NR)の調製と成長メカニズム , 2003 .

[40]  S. Nugen,et al.  Nanoimprinted Patterned Pillar Substrates for Surface-Enhanced Raman Scattering Applications. , 2015, ACS applied materials & interfaces.

[41]  Huan‐Tsung Chang,et al.  Detection of mercury(II) ions using colorimetric gold nanoparticles on paper-based analytical devices. , 2014, Analytical chemistry.