Sacrificial polymers for nanofluidic channels in biological applications

Chip based bio/chemical analysis relies on networks of fluidic channels that are connected to reaction chambers and sensors. For sensitive detection it is important to scale down the size of the channels so that they approach the relevant length scales of the molecules of interest. Here we have made sealed channels on the 100 nm scale using nanoimprinting to pattern the sacrificial polymer polynorbornene over areas of several square centimetres. We have combined channels of different cross sections and we have shown that the nanochannels can be made hydrophilic with DNA transported electrophoretically in these self-sealed channels.

[1]  S S Chan,et al.  Separation of 100-kilobase DNA molecules in 10 seconds. , 2001, Analytical chemistry.

[2]  大房 健.  基礎講座 電気泳動(Electrophoresis) , 2005 .

[3]  Robert A. Shick,et al.  Functionalized polynorbornene dielectric polymers: Adhesion and mechanical properties , 1999 .

[4]  H. Craighead,et al.  Separation of long DNA molecules in a microfabricated entropic trap array. , 2000, Science.

[5]  S S Chan,et al.  Sorting by diffusion: an asymmetric obstacle course for continuous molecular separation. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[6]  H. Craighead,et al.  Heat-depolymerizable polycarbonates as electron beam patternable sacrificial layers for nanofluidics , 2001 .

[7]  P. Colinvaux,et al.  A Long Pollen Record from Lowland Amazonia: Forest and Cooling in Glacial Times , 1996, Science.

[8]  J. Legrange,et al.  Dependence of DNA helix flexibility on base composition , 1983, Nature.

[9]  S. Quake,et al.  From micro- to nanofabrication with soft materials. , 2000, Science.

[10]  V W Weedn,et al.  Real-time microchip PCR for detecting single-base differences in viral and human DNA. , 1998, Analytical chemistry.

[11]  P. Kohl,et al.  Fabrication of air-channel structures for microfluidic, microelectromechanical, and microelectronic applications , 2001 .

[12]  Stephen Y. Chou,et al.  Imprint of sub-25 nm vias and trenches in polymers , 1995 .

[13]  H. Carlson,et al.  Sorting of White Blood Cells in a Lattice , 1997 .

[14]  P. Kohl,et al.  Fabrication of microchannels using polycarbonates as sacrificial materials , 2001 .

[15]  R. Austin,et al.  Design of a microfabricated magnetic cell separator , 2001, Electrophoresis.

[16]  Robert H. Austin,et al.  Fabrication of 10 nm enclosed nanofluidic channels , 2002 .

[17]  H. McAdams,et al.  Global analysis of the genetic network controlling a bacterial cell cycle. , 2000, Science.

[18]  S. Chou,et al.  Imprint Lithography with 25-Nanometer Resolution , 1996, Science.