Morphological , anatomical , macro and micromolecular markers for Solanum cernuum identi cation Tânia

The plants consumed as remedy by the population may have imprecise taxonomical identi cation. If these plants are used for the production of phytomedicines such misidenti cation may affect the quality of the product. Hereby, we describe markers for identi cation of the entire plant or grounded plant material or the crude extract of Solanum cernuum Vell. (Solanaceae). Specimens from four localities were collected, analyzed and compared. Morphological characters were used to identify the plant when it is not grounded or extracted. However, when the plant material is grounded, the set of trichomes may be used as anatomical marker. The region ITS1, 5.8S and ITS2 of the nuclear ribosomal DNA was cloned and sequenced. The sequence, with length of about 600 base pairs, being 48.1% AT , was deposited in GenBank under the accession number DQ837371. Once this sequence is speci c to S. cernuum, it was used as marker for this species. For the crude extract, chromatographic pro les of the leaves extracts were obtained by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Two avonoids were isolated and identi ed as quercitrin and afzelin. So, this study presents morphological, anatomical, macro and micromolecular markers to identify S. cernuum.

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