Platelet Anti‐BAKb Antibody Associated with Neonatal Alloimmune Thrombocytopenia

Abstract. A platelet alloantibody of IgG class but unknown specificity was detected in the serum from the mother of an infant with neonatal alloimmune thrombocytopenia. The antibody reacted by indirect immunofluorescence with 33/65 random donors, 10/11 Baka‐negative donors and 23/54 Baka‐positive donors. The mother's platelet phenotype was PLA1, Koa, Kob, Baka and the father's was PLA1, Koa, Kob, Baka. Immunoblotting and immunoprecipitation of maternal serum with paternal platelets produced a band at molecular weight 140 kilodaltons identical to the band obtained with Baka antiserum. Family studies confirmed the allelic distribution of Baka and the unknown antigen. The platelet‐specific antibody in this patient meets the criteria for an antibody to the new platelet antigen, Bakb.

[1]  R. Aster,et al.  Identification of Bakb, a new platelet-specific antigen associated with posttransfusion purpura , 1988 .

[2]  K.,et al.  Identification of Bakb, a new platelet-specific antigen associated with posttransfusion purpura. , 1988, Blood.

[3]  A. Borne,et al.  Characterization of platelet‐specific alloantigens by immimoblotting: localization of Zw and Bak antigens , 1986, British journal of haematology.

[4]  L. D. de Waal,et al.  Alloimmunization against the platelet-specific Zwa antigen, resulting in neonatal alloimmune thrombocytopenia or posttransfusion purpura, is associated with the supertypic DRw52 antigen including DR3 and DRw6. , 1986, Human immunology.

[5]  P. D. de Groot,et al.  Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa. , 1985, The Journal of biological chemistry.

[6]  R. Nordhagen,et al.  Chloroquine Removal of HLA Antigens from Platelets for the Platelet Immunofluorescence Test , 1985, Vox sanguinis.

[7]  A. Mulder,et al.  Immunochemical characterization of platelet-specific alloantigens. , 2009, Scandinavian journal of haematology.

[8]  F. Verheugt,et al.  Baka, a New Platelet‐Specific Antigen Involved in Neonatal Allo‐Immune Thrombocytopenia , 1980, Vox sanguinis.

[9]  F. Oosterhof,et al.  A Simple Immunofluorescence Test for the Detection of Platelet Antibodies , 1978, British journal of haematology.

[10]  P. Terasaki,et al.  Microdroplet testing for HLA-A, -B, -C, and -D antigens. The Phillip Levine Award Lecture. , 1978, American journal of clinical pathology.

[11]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.