Posttranslational of ¢t-Tubulin: Acetylated and Detyrosinated Forms in Axons of Rat Cerebellum

The distribution of acetylated ct-tubulin in rat cerebellum was examined and compared with that of total ct-tubulin and tyrosinated ¢t-tubulin. From immunoperoxidase-stained vibratome sections of rat cerebellum it was found that acetylated a-tubulin, de- tectable with monoclonal 6-11B-l, was preferentially enriched in axons compared with dendrites. Parallel fiber axons, in particular, were labeled with 6-11B-1 yet unstained by an antibody recognizing tyrosinated ¢t-tubulin, indicating that parallel fibers contain ¢t-tu- bulin that is acetylated and detyrosinated. Axonal microtubules are known to be highly stable and the distribution of acetylated ct-tubulin in other classes of stable microtubules suggests that acetylation and possi- bly detyrosination may play a role in the maintenance of stable populations of microtubules.

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