Multi-components of T2 relaxation in ex vivo cartilage and tendon.

The multi-components of T2 relaxation in cartilage and tendon were investigated by microscopic MRI (microMRI) at 13 and 26 microm transverse resolutions. Two imaging protocols were used to quantify T2 relaxation in the specimens, a 5-point sampling and a 60-point sampling. Both multi-exponential and non-negative-least-square (NNLS) fitting methods were used to analyze the microMRI signal. When the imaging voxel size was 6.76 x 10(-4)mm3 and within the limit of practical signal-to-noise ratio (SNR) in microscopic imaging experiments, we found that (1) canine tendon has multiple T2 components; (2) bovine nasal cartilage has a single T2 component; and (3) canine articular cartilage has a single T2 component. The T2 profiles from both 5-point and 60-point methods were found to be consistent in articular cartilage. In addition, the depletion of the glycosaminoglycan component in cartilage by the trypsin digestion method was found to result in a 9.81-20.52% increase in T2 relaxation in articular cartilage, depending upon the angle at which the tissue specimen was oriented in the magnetic field.

[1]  G. Lust,et al.  Origin of cartilage laminae in MRI , 1997, Journal of magnetic resonance imaging : JMRI.

[2]  L H Kidder,et al.  Imaging of collagen and proteoglycan in cartilage sections using Fourier transform infrared spectral imaging. , 2001, Arthritis and rheumatism.

[3]  I. Clarke,et al.  Articular cartilage: a review and scanning electron microscope study. 1. The interterritorial fibrillar architecture. , 1971, The Journal of bone and joint surgery. British volume.

[4]  Hisham A Alhadlaq,et al.  Orientational dependence of T2 relaxation in articular cartilage: A microscopic MRI (μMRI) study , 2002, Magnetic resonance in medicine.

[5]  S. Nelson,et al.  Measurement of in vivo multi-component T2 relaxation times for brain tissue using multi-slice T2 prep at 1.5 and 3 T. , 2006, Magnetic resonance imaging.

[6]  P. Gillis,et al.  Fiber-to-field angle dependence of proton nuclear magnetic relaxation in collagen. , 1990, Magnetic resonance imaging.

[7]  S. Swanson,et al.  A three-component model for magnetization transfer. Solution by projection-operator technique, and application to cartilage. , 1996, Journal of magnetic resonance. Series B.

[8]  Alex L. MacKay,et al.  Quantitative interpretation of NMR relaxation data , 1989 .

[9]  I. Clarke.  Articular cartilage: a review and scanning electron microscope study. II. The territorial fibrillar architecture. , 1974, Journal of anatomy.

[10]  G. Fullerton,et al.  Orientation of tendons in the magnetic field and its effect on T2 relaxation times. , 1985, Radiology.

[11]  M. Bronskill,et al.  Variation in measured transverse relaxation in tissue resulting from spin locking with the CPMG sequence , 1988 .

[12]  Y. Xia,et al.  Magic-Angle Effect in Magnetic Resonance Imaging of Articular Cartilage: A Review , 2000, Investigative radiology.

[13]  A. Maroudas,et al.  Methods in cartilage research , 1990 .

[14]  Y. Xia,et al.  The depth-dependent anisotropy of articular cartilage by Fourier-transform infrared imaging (FTIRI). , 2007, Osteoarthritis and cartilage.

[15]  Garry E Gold,et al.  Dynamic and Functional Imaging of the Musculoskeletal System , 2003, Seminars in musculoskeletal radiology.

[16]  H. Rechl,et al.  Structure, function, and degeneration of bovine hyaline cartilage: assessment with MR imaging in vitro. , 1989, Radiology.

[17]  R Mendelsohn,et al.  FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. , 2001, Biopolymers.

[18]  Charles L. Lawson,et al.  Solving least squares problems , 1976, Classics in applied mathematics.

[19]  A. Maroudas,et al.  Biophysical chemistry of cartilaginous tissues with special reference to solute and fluid transport. , 1975, Biorheology.

[20]  C. Glüer,et al.  Detection of changes in cartilage water content using MRI T2-mapping in vivo. , 2002, Osteoarthritis and cartilage.

[21]  Yang Xia,et al.  Analysis of multi-exponential relaxation data with very short components using linear regularization. , 2004, Journal of magnetic resonance.

[22]  G. Ling.  Studies on the physical state of water in living cells and model systems. VII. Exclusion of sugars and sugar alcohols from the water in sulfonate ion exchange resins: the "size rule". , 1987, Physiological chemistry and physics and medical NMR.

[23]  Y. Xia,et al.  Characteristics of topographical heterogeneity of articular cartilage over the joint surface of a humeral head. , 2002, Osteoarthritis and cartilage.

[24]  J R Matyas,et al.  Detecting structural changes in early experimental osteoarthritis of tibial cartilage by microscopic magnetic resonance imaging and polarised light microscopy , 2004, Annals of the rheumatic diseases.

[25]  John C. Gore,et al.  Quantitative T2 measurement of a single voxel with arbitrary shape using pinwheel excitation and CPMG acquisition , 2007, Magnetic Resonance Materials in Physics, Biology and Medicine.

[26]  A. Maroudas,et al.  Structure of proteoglycans from different layers of human articular cartilage. , 1983, The Biochemical journal.

[27]  Y. Xia,et al.  Relaxation anisotropy in cartilage by NMR microscopy (muMRI) at 14-microm resolution. , 1998, Magnetic resonance in medicine.

[28]  J Silvennoinen,et al.  T2 relaxation reveals spatial collagen architecture in articular cartilage: A comparative quantitative MRI and polarized light microscopic study , 2001, Magnetic resonance in medicine.

[29]  B. Rutt,et al.  In vivo measurements of multi-component T2 relaxation behaviour in guinea pig brain. , 1999, Magnetic resonance imaging.

[30]  X. Edward Guo,et al.  Mechano-electrochemical properties of articular cartilage: their inhomogeneities and anisotropies. , 2002, Annual review of biomedical engineering.

[31]  Van,et al.  Spatial variation of T2 in human articular cartilage. , 1997, Radiology.

[32]  Albert Macovski,et al.  MR Spectroscopic imaging of collagen: Tendons and knee menisci , 1995, Magnetic resonance in medicine.

[33]  P. Torzilli,et al.  Proton spin-spin relaxation study of molecular dynamics and proteoglycan hydration in articular cartilage. , 2000, Biomaterials.

[34]  R M Henkelman,et al.  Diffusional anisotropy of T2 components in bovine optic nerve , 1998, Magnetic resonance in medicine.

[35]  W Gründer,et al.  MR‐microscopic visualization of anisotropic internal cartilage structures using the magic angle technique , 1998, Magnetic resonance in medicine.

[36]  J Silvennoinen,et al.  Quantitative MR microscopy of enzymatically degraded articular cartilage , 2000, Magnetic resonance in medicine.

[37]  T J Mosher,et al.  Human articular cartilage: influence of aging and early symptomatic degeneration on the spatial variation of T2--preliminary findings at 3 T. , 2000, Radiology.

[38]  M. Bronskill,et al.  Analysis of discrete T2 components of NMR relaxation for aqueous solutions in hollow fiber capillaries , 1994, Magnetic resonance in medicine.

[39]  H. Kresse,et al.  Light and electron microscopical immunohistochemical localization of the small proteoglycan core proteins decorin and biglycan in human knee joint cartilage , 2004, The Histochemical Journal.

[40]  G. Marsh,et al.  Multicomponent T2 relaxation of in vivo skeletal muscle , 1999, Magnetic resonance in medicine.

[41]  P Olivier,et al.  Structural evaluation of articular cartilage: potential contribution of magnetic resonance techniques used in clinical practice. , 2001, Arthritis and rheumatism.

[42]  Y. Hirasawa,et al.  Characteristics of proton NMR T(2) relaxation of water in the normal and regenerating tendon. , 2000, The Japanese journal of physiology.

[43]  N. Murase,et al.  Multiexponential proton relaxation processes of compartmentalized water in gels , 1992, Magnetic resonance in medicine.

[44]  J L Potter,et al.  NMR relaxation of protons in tissues and other macromolecular water solutions. , 1982, Magnetic resonance imaging.

[45]  L W Jelinski,et al.  Self-diffusion monitors degraded cartilage. , 1995, Archives of biochemistry and biophysics.

[46]  A. Maroudas,et al.  Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. I. Chemical composition. , 1977, Annals of the rheumatic diseases.

[47]  V. Mlynárik,et al.  Transverse relaxation mechanisms in articular cartilage. , 2004, Journal of magnetic resonance.

[48]  G. Navon,et al.  Multinuclear NMR and MRI studies of the maturation of pig articular cartilage , 2006, Magnetic resonance in medicine.

[49]  Y. Xia,et al.  Quantitative in situ correlation between microscopic MRI and polarized light microscopy studies of articular cartilage. , 2001, Osteoarthritis and cartilage.

[50]  Yang Xia,et al.  Imaging the physical and morphological properties of a multi‐zone young articular cartilage at microscopic resolution , 2003, Journal of magnetic resonance imaging : JMRI.

[51]  R E Snyder,et al.  T2 relaxation of peripheral nerve measured in vivo. , 1995, Magnetic resonance imaging.

[52]  K. Marshall,et al.  Macromolecule and water magnetization exchange modeling in articular cartilage , 2000, Magnetic resonance in medicine.

[53]  C. Lawson,et al.  Solving least squares problems , 1976, Classics in applied mathematics.

[54]  V. Mlynárik,et al.  Investigation of laminar appearance of articular cartilage by means of magnetic resonance microscopy. , 1996, Magnetic resonance imaging.

[55]  M. Bronskill,et al.  Anisotropy of NMR properties of tissues , 1994, Magnetic resonance in medicine.

[56]  Peter T Fox,et al.  Three‐pool model of white matter , 2003, Journal of magnetic resonance imaging : JMRI.

[57]  R. Meyer,et al.  Pixel T2 distribution in functional magnetic resonance images of muscle. , 1999, Journal of applied physiology.

[58]  W Gründer,et al.  Action of Compression and Cations on the Proton and Deuterium Relaxation in Cartilage , 1995, Magnetic resonance in medicine.