Maize Haploid Identification via LSTM-CNN and Hyperspectral Imaging Technology

Accurate and fast identification of seed cultivars is crucial to plant breeding, with accelerating breeding of new products and increasing its quality. In our study, the first attempt to design a high-accurate identification model of maize haploid seeds from diploid ones based on optimum waveband selection of the LSTM-CNN algorithm is realized via deep learning and hyperspectral imaging technology, with accuracy reaching 97% in the determining optimum waveband of 1367.6-1526.4nm. The verification of testing another cultivar achieved an accuracy of 93% in the same waveband. The model collected images of 256 wavebands of seeds in the spectral region of 862.9-1704.2nm. The high-noise waveband intervals were found and deleted by the LSTM. The optimum-data waveband intervals were determined by CNN's waveband-based detection. The optimum sample set for network training only accounted for 1/5 of total sample data. The accuracy was significantly higher than the full-waveband modeling or modeling of any other wavebands. Our study demonstrates that the proposed model has outstanding effect on maize haploid identification and it could be generalized to some extent.

[1]  Yan Wang,et al.  Genetic purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata) , 2013 .

[2]  Colm P. O'Donnell,et al.  Hyperspectral imaging – an emerging process analytical tool for food quality and safety control , 2007 .

[3]  Min Huang,et al.  Classification of maize seeds of different years based on hyperspectral imaging and model updating , 2016, Comput. Electron. Agric..

[4]  Peter Stamp,et al.  Inducer line generated double haploid seeds for combined waxy and opaque 2 grain quality in subtropical maize (Zea mays. L.) , 2011, Euphytica.

[5]  Fang Cheng,et al.  Spectral and Image Integrated Analysis of Hyperspectral Data for Waxy Corn Seed Variety Classification , 2015, Sensors.

[6]  P. Leroy,et al.  Molecular and morphological evaluation of doubled haploid lines in maize. 1. Homogeneity within DH lines , 1993, Theoretical and Applied Genetics.

[7]  S. Emamgholizadeh,et al.  Seed yield prediction of sesame using artificial neural network , 2015 .

[8]  Digvir S. Jayas,et al.  Hyperspectral imaging to classify and monitor quality of agricultural materials , 2015 .

[9]  Daniel Rivero,et al.  Using genetic algorithms and k-nearest neighbour for automatic frequency band selection for signal classification , 2012, IET Signal Process..

[10]  Corinna Cortes,et al.  Support-Vector Networks , 1995, Machine Learning.

[11]  Indra D. Bhatt,et al.  Characterization of Agro-diversity by Seed Storage Protein Electrophoresis: Focus on Rice Germplasm from Uttarakhand Himalaya, India , 2010 .

[12]  Qingming Huang,et al.  Fully-Automated High-Throughput NMR System for Screening of Haploid Kernels of Maize (Corn) by Measurement of Oil Content , 2016, PloS one.

[13]  Albrecht E. Melchinger,et al.  Doubled Haploids in Tropical Maize: I. Effects of Inducers and Source Germplasm on in vivo Haploid Induction Rates , 2011 .

[14]  J. O. Rawlings,et al.  Appropriate characters for racial classification in maize , 2008, Economic Botany.

[15]  Chu Zhang,et al.  Rice Seed Cultivar Identification Using Near-Infrared Hyperspectral Imaging and Multivariate Data Analysis , 2013, Sensors.

[16]  S. Chalyk,et al.  The Use of Matroclinous Maize Haploids for Recurrent Selection , 2001, Russian Journal of Genetics.

[17]  J. Eder,et al.  In vivo haploid induction in maize , 2002, Theoretical and Applied Genetics.

[18]  Liu Zhi,et al.  The Breeding and Identification of Haploid Inducer with High Frequ ency Parthenogenesis in Maize , 2000 .

[19]  Dong Xu,et al.  NOISE ESTIMATION OF HYPERSPECTRAL REMOTE SENSING IMAGE BASED ON MULTIPLE LINEAR REGRESSION AND WAVELET TRANSFORM , 2013 .

[20]  Radford M. Neal Pattern Recognition and Machine Learning , 2007, Technometrics.

[21]  Pengcheng Nie,et al.  Spectral Multivariable Selection and Calibration in Visible-Shortwave Near-Infrared Spectroscopy for Non-Destructive Protein Assessment of Spirulina Microalga Powder , 2013 .

[22]  Kang Tu,et al.  Measurement of moisture, soluble solids, sucrose content and mechanical properties in sugar beet using portable visible and near-infrared spectroscopy , 2015 .

[23]  Noel D.G. White,et al.  Feasibility of near-infrared hyperspectral imaging to differentiate Canadian wheat classes , 2008 .

[24]  Albrecht E. Melchinger,et al.  Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize , 2013, Scientific Reports.

[25]  Myong Kee Jeong,et al.  An evolutionary algorithm with the partial sequential forward floating search mutation for large-scale feature selection problems , 2015, J. Oper. Res. Soc..

[26]  Jürgen Schmidhuber,et al.  Learning Precise Timing with LSTM Recurrent Networks , 2003, J. Mach. Learn. Res..

[27]  Bernhard E. Boser,et al.  A training algorithm for optimal margin classifiers , 1992, COLT '92.

[28]  Caroline Fossati,et al.  Nonwhite Noise Reduction in Hyperspectral Images , 2012, IEEE Geoscience and Remote Sensing Letters.

[29]  Yong He,et al.  Application of Hyperspectral Imaging and Chemometric Calibrations for Variety Discrimination of Maize Seeds , 2012, Sensors.

[30]  Renfu Lu,et al.  Hyperspectral Imaging-Based Classification and Wavebands Selection for Internal Defect Detection of Pickling Cucumbers , 2013, Food and Bioprocess Technology.

[31]  Honglak Lee,et al.  An Analysis of Single-Layer Networks in Unsupervised Feature Learning , 2011, AISTATS.

[32]  Birte Boelt,et al.  Classification of Viable and Non-Viable Spinach (Spinacia Oleracea L.) Seeds by Single Seed near Infrared Spectroscopy and Extended Canonical Variates Analysis , 2011 .