Impact of food matrix on isoflavone metabolism and cardiovascular biomarkers in adults with hypercholesterolemia.

The role of food matrix and gender on soy isoflavone metabolism and biomarkers of activity were examined in twenty free-living adults (34.7 ± 11.5 years old) with hypercholesterolemia (221.9 ± 18.7mg dL(-1)). In a randomized crossover design study, participants consumed soy bread (3 wk) or a soy beverage (3 wk) containing 20 g soy protein with 99 and 93 mg isoflavones aglycone equivalents per day, respectively. During soy bread intervention, women had significantly greater microbial metabolite excretion (P = 0.05) of isoflavonoids than men. In men, isoflavone metabolite excretion was not discernibly different between the two matrices. Significant reductions (P≤ 0.05) in triglycerides (24.8%), LDL cholesterol (6.0%), apolipoprotein A-I (12.3%), and lipid oxidative stress capacity (25.5%), were observed after soy food intervention. Our findings suggest that the food matrix significantly impacts soy isoflavone metabolism, particularly microbial metabolites in women.

[1]  L. Wilkens,et al.  Apparent bioavailability of isoflavones after intake of liquid and solid soya foods , 2009, British Journal of Nutrition.

[2]  C. Takimoto,et al.  Dietary genistein inhibits metastasis of human prostate cancer in mice. , 2008, Cancer research.

[3]  F. Welty,et al.  Effect of soy nuts on blood pressure and lipid levels in hypertensive, prehypertensive, and normotensive postmenopausal women. , 2007, Archives of internal medicine.

[4]  R. Karas,et al.  Effect of soy protein from differently processed products on cardiovascular disease risk factors and vascular endothelial function in hypercholesterolemic subjects. , 2007, The American journal of clinical nutrition.

[5]  A. Wu,et al.  Estimated Asian Adult Soy Protein and Isoflavone Intakes , 2006, Nutrition and cancer.

[6]  J. Barrett.  The Science of Soy: What Do We Really Know? , 2006, Environmental health perspectives.

[7]  Huihua Huang,et al.  Effect of thermal processing on genistein, daidzein and glycitein content in soymilk , 2006 .

[8]  B. Trock,et al.  Meta-analysis of soy intake and breast cancer risk. , 2006, Journal of the National Cancer Institute.

[9]  J. Hallund,et al.  Absorption of isoflavones in humans: effects of food matrix and processing. , 2006, The Journal of nutritional biochemistry.

[10]  A. McTiernan,et al.  Postmenopausal bone mineral density in relation to soy isoflavone-metabolizing phenotypes. , 2006, Maturitas.

[11]  M. Riaz,et al.  Soyfoods: market and products. , 2006 .

[12]  K. Setchell,et al.  Factors affecting the bioavailability of soy isoflavones in humans after ingestion of physiologically relevant levels from different soy foods. , 2006, The Journal of nutrition.

[13]  J. Boye,et al.  Soybean isoflavones: Efficacy of extraction conditions and effect of food type on extractability , 2005 .

[14]  D. Moore,et al.  Molecular mechanisms of action of the soy isoflavones includes activation of promiscuous nuclear receptors. A review. , 2005, The Journal of nutritional biochemistry.

[15]  J. Lampe,et al.  Gut Bacterial Metabolism of the Soy Isoflavone Daidzein: Exploring the Relevance to Human Health , 2005, Experimental biology and medicine.

[16]  J. Thrasher,et al.  The role of soy phytoestrogens in prostate cancer , 2005, Current opinion in urology.

[17]  M. Messina.  Western soy intake is too low to produce health effects. , 2004, The American journal of clinical nutrition.

[18]  Jonathan E. Brown,et al.  Urinary isoflavone kinetics: the effect of age, gender, food matrix and chemical composition , 2004, British Journal of Nutrition.

[19]  A. Pantuck,et al.  Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents. , 2004, Japanese journal of clinical oncology.

[20]  H. Adlercreutz,et al.  Metabolism of the soy isoflavones daidzein, genistein and glycitein in human subjects. Identification of new metabolites having an intact isoflavonoid skeleton , 2003, The Journal of Steroid Biochemistry and Molecular Biology.

[21]  D. Kostelac,et al.  Phytoestrogens modulate binding response of estrogen receptors α and β to the estrogen response element , 2003 .

[22]  C. Takimoto,et al.  Phase I pharmacokinetic and pharmacodynamic analysis of unconjugated soy isoflavones administered to individuals with cancer. , 2003, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[23]  P. Murphy,et al.  Rapid gut transit time and slow fecal isoflavone disappearance phenotype are associated with greater genistein bioavailability in women. , 2003, The Journal of nutrition.

[24]  K. Setchell,et al.  Dietary phytoestrogens and their effect on bone: evidence from in vitro and in vivo, human observational, and dietary intervention studies. , 2003, The American journal of clinical nutrition.

[25]  V. Irribarra,et al.  Isolated soy protein improves endothelial function in postmenopausal hypercholesterolemic women , 2003, European Journal of Clinical Nutrition.

[26]  A. Villablanca,et al.  Soy protein with isoflavones has favorable effects on endothelial function that are independent of lipid and antioxidant effects in healthy postmenopausal women. , 2003, The American journal of clinical nutrition.

[27]  I. Shaw,et al.  Bioactive isoflavones in functional foods: the importance of gut microflora on bioavailability. , 2003, Nutrition reviews.

[28]  S. Clinton,et al.  The consumption of processed tomato products enhances plasma lycopene concentrations in association with a reduced lipoprotein sensitivity to oxidative damage. , 2003, The Journal of nutrition.

[29]  G. Rechkemmer,et al.  Phytoestrogens modulate binding response of estrogen receptors alpha and beta to the estrogen response element. , 2003, Journal of agricultural and food chemistry.

[30]  K. Setchell,et al.  The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones. , 2002, The Journal of nutrition.

[31]  M. Schell,et al.  Safety and pharmacokinetics of purified soy isoflavones: single-dose administration to postmenopausal women. , 2002, The American journal of clinical nutrition.

[32]  P. Walsh.  Risks and benefits of soy phytoestrogens in cardiovascular diseases, cancer, climacteric symptoms and osteoporosis. , 2002, The Journal of urology.

[33]  L. Kolonel,et al.  Liquid chromatographic-photodiode array mass spectrometric analysis of dietary phytoestrogens from human urine and blood. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[34]  P. Murphy,et al.  Solvent extraction selection in the determination of isoflavones in soy foods. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[35]  M. Kurzer.  Hormonal effects of soy in premenopausal women and men. , 2002, The Journal of nutrition.

[36]  W. Heizer,et al.  Clinical characteristics and pharmacokinetics of purified soy isoflavones: single-dose administration to healthy men. , 2002, The American journal of clinical nutrition.

[37]  A. Oto,et al.  Soy protein diet significantly improves endothelial function and lipid parameters , 2000, Clinical cardiology.

[38]  Z. Djuric,et al.  Effect of soy isoflavone supplementation on markers of oxidative stress in men and women. , 2001, Cancer letters.

[39]  A. Hossaini,et al.  Estrogenic activity of flavonoids in mice. The importance of estrogen receptor distribution, metabolism and bioavailability. , 2000, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[40]  A. Duncan,et al.  Premenopausal equol excretors show plasma hormone profiles associated with lowered risk of breast cancer. , 2000, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[41]  J. Cooke,et al.  Phytoestrogens and cardiovascular health. , 2000, Journal of the American College of Cardiology.

[42]  P. Murphy,et al.  Neither background diet nor type of soy food affects short-term isoflavone bioavailability in women. , 2000, The Journal of nutrition.

[43]  H. Adlercreutz,et al.  Identification of isoflavone metabolites dihydrodaidzein, dihydrogenistein, 6'-OH-O-dma, and cis-4-OH-equol in human urine by gas chromatography-mass spectroscopy using authentic reference compounds. , 1999, Analytical biochemistry.

[44]  J. Crouse,et al.  A randomized trial comparing the effect of casein with that of soy protein containing varying amounts of isoflavones on plasma concentrations of lipids and lipoproteins. , 1999, Archives of internal medicine.

[45]  M. Messina.  Legumes and soybeans: overview of their nutritional profiles and health effects. , 1999, The American journal of clinical nutrition.

[46]  P. Murphy,et al.  Urinary disposition of the soybean isoflavones daidzein, genistein and glycitein differs among humans with moderate fecal isoflavone degradation activity. , 1999, The Journal of nutrition.

[47]  K. Setchell,et al.  Dietary isoflavones: biological effects and relevance to human health. , 1999, The Journal of nutrition.

[48]  T. Sanders,et al.  Metabolism of oestrogens and phytoestrogens: role of the gut microflora. , 1999, Biochemical Society transactions.

[49]  S. Barnes,et al.  Chemical modification of isoflavones in soyfoods during cooking and processing. , 1998, The American journal of clinical nutrition.

[50]  K. Anderson,et al.  Sex and long-term soy diets affect the metabolism and excretion of soy isoflavones in humans. , 1998, The American journal of clinical nutrition.

[51]  M. Anthony,et al.  Effects of soy isoflavones on atherosclerosis: potential mechanisms. , 1998, The American journal of clinical nutrition.

[52]  G. Gordon,et al.  Soy, its components, and cancer prevention: a review of the in vitro, animal, and human data. , 1998, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[53]  William L. Haskell,et al.  Potential Health Benefits of Dietary Phytoestrogens: A Review of the Clinical, Epidemiological, and Mechanistic Evidence1 , 1998 .

[54]  R. A. King,et al.  Plasma and urinary kinetics of the isoflavones daidzein and genistein after a single soy meal in humans. , 1998, The American journal of clinical nutrition.

[55]  S. Ojala,et al.  Effect of soybean phytoestrogen intake on low density lipoprotein oxidation resistance. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[56]  C D Gardner,et al.  Clinical review 97: Potential health benefits of dietary phytoestrogens: a review of the clinical, epidemiological, and mechanistic evidence. , 1998, The Journal of clinical endocrinology and metabolism.

[57]  P. Murphy,et al.  Mass Balance Study of Isoflavones during Soybean Processing , 1996 .

[58]  P. Wilson,et al.  Reference intervals for plasma apolipoprotein A-1 determined with a standardized commercial immunoturbidimetric assay: results from the Framingham Offspring Study. , 1996, Clinical chemistry.

[59]  K. Anderson,et al.  Altered kinetics and extent of urinary daidzein and genistein excretion in women during chronic soya exposure. , 1996, Nutrition and cancer.

[60]  P. Wilson,et al.  Reference intervals for plasma apolipoprotein B determined with a standardized commercial immunoturbidimetric assay: results from the Framingham Offspring Study. , 1996, Clinical chemistry.

[61]  M. Nair,et al.  Metabolism of daidzein and genistein by intestinal bacteria. , 1995, Journal of natural products.

[62]  P. Murphy,et al.  Bioavailability of soybean isoflavones depends upon gut microflora in women. , 1995, The Journal of nutrition.

[63]  M. Waring,et al.  A urinary profile study of dietary phytoestrogens. The identification and mode of metabolism of new isoflavonoids , 1995, The Journal of Steroid Biochemistry and Molecular Biology.

[64]  P. Golbitz.  Traditional soyfoods: processing and products. , 1995, The Journal of nutrition.

[65]  M. Waring,et al.  The Variable Metabolic Response to Dietary Isoflavones in Humans , 1995, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[66]  S. Barnes,et al.  Rationale for the use of genistein‐containing soy matrices in chemoprevention trials for breast and prostate cancer , 1995, Journal of cellular biochemistry. Supplement.

[67]  P. Murphy,et al.  Isoflavone content in commercial soybean foods , 1994 .

[68]  S. Barnes,et al.  Genistein, daidzein, and their .beta.-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets , 1993 .

[69]  H. Adlercreutz.  Western diet and Western diseases: some hormonal and biochemical mechanisms and associations. , 1990, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[70]  J. Dilawari,et al.  Dietary fibres, fibre analogues, and glucose tolerance: importance of viscosity. , 1978, British medical journal.

[71]  P. Fu,et al.  Enzymatic determination of total serum cholesterol. , 1974, Clinical chemistry.

[72]  G Bucolo,et al.  Quantitative determination of serum triglycerides by the use of enzymes. , 1973, Clinical chemistry.

[73]  R. Levy,et al.  Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. , 1972, Clinical chemistry.