Phenolic compounds and antioxidant activity of blueberry cultivars grown in Brazil

O objetivo deste trabalho foi avaliar a concentracao de compostos fenolicos totais, antocianinas monomericas totais e a atividade antioxidante in vitro das principais cultivares de mirtilo (Vaccinium sp.) produzidas no Brasil. O metodo Folin-Ciocalteau foi aplicado para quantificar os compostos fenolicos totais e os metodos ABTS, DPPH, FRAPe β-caroteno/acido linoleico para avaliar a atividade antioxidante. O teor de compostos fenolicos totais encontrado variou de 274,48 a 694,60 mg de equivalente a acido galico (EAG).100 g-1 em peso fresco (PF). A concentracao de antocianinas monomericas variou da cultivar Bluecrop ate a Tifblue, com valores de, respectivamente, 40,62 a 378,31 mg.100 g-1 PF. A atividade antioxidante medida pelos metodos ABTS, DPPH e FRAP mostrou grande amplitude entre as cultivares estudadas, com valores variando de, respectivamente, 1238,48 a 2445,96, 1014,24 a 2055,06 e 699,78 a 1740,25 µmol TEAC.100 g-1 PF. Os resultados confirmam o mirtilo como fonte de compostos fenolicos com elevada atividade antioxidante. Demonstram ainda que existem diferentes niveis de concentracoes de compostos fenolicos e atividade antioxidante de acordo com a cultivar e o local de producao desta fruta.

[1]  S. Buratti,et al.  Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties , 2009 .

[2]  D. Ingram,et al.  A blueberry-enriched diet provides cellular protection against oxidative stress and reduces a kainate-induced learning impairment in rats , 2008, Neurobiology of Aging.

[3]  R. Liu,et al.  Cellular antioxidant activity of common fruits. , 2008, Journal of agricultural and food chemistry.

[4]  T. Finkel,et al.  Free radicals and senescence. , 2008, Experimental cell research.

[5]  N. Seeram Berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease. , 2008, Journal of agricultural and food chemistry.

[6]  M. Camire,et al.  Characteristics of wild blueberry–soy beverages , 2007 .

[7]  C. Akoh,et al.  Effect of storage conditions on the biological activity of phenolic compounds of blueberry extract packed in glass bottles. , 2007, Journal of agricultural and food chemistry.

[8]  R. Mateos,et al.  Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to macromolecules (DNA, lipids, and proteins). , 2007, Journal of separation science.

[9]  G. Beecher,et al.  Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. , 2004, Journal of agricultural and food chemistry.

[10]  J. Exon,et al.  Content and profile of flavanoid and phenolic acid compounds in conjunction with the antioxidant capacity for a variety of northwest Vaccinium berries. , 2004, Journal of agricultural and food chemistry.

[11]  J. Rivas-Gonzalo,et al.  Evaluation of the antioxidant properties of fruits , 2004 .

[12]  Shiow Y. Wang,et al.  Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. , 2003, Journal of agricultural and food chemistry.

[13]  P. Kefalas,et al.  Correlation of Pigment and Flavanol Content with Antioxidant Properties in Selected Aged Regional Wines from Greece , 2002 .

[14]  C. Akoh,et al.  Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries. , 2002, Journal of agricultural and food chemistry.

[15]  R. Wrolstad,et al.  Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. , 2002, Journal of agricultural and food chemistry.

[16]  J. Luby,et al.  Genotypic and Environmental Variation in Antioxidant Activity, Total Phenolic Content, and Anthocyanin Content among Blueberry Cultivars , 2002 .

[17]  Charanjit Kaur,et al.  Antioxidants in fruits and vegetables – the millennium’s health , 2001 .

[18]  R. Wrolstad,et al.  Characterization and Measurement of Anthocyanins by UV‐Visible Spectroscopy , 2001 .

[19]  C. S. Yang,et al.  Inhibition of carcinogenesis by dietary polyphenolic compounds. , 2001, Annual review of nutrition.

[20]  J. German,et al.  The health benefits of wine. , 2003, Annual review of nutrition.

[21]  A. Crozier,et al.  Plant-derived phenolic antioxidants , 2000, Current opinion in clinical nutrition and metabolic care.

[22]  C. Forney,et al.  Antioxidant capacity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits. , 1999, Journal of agricultural and food chemistry.

[23]  C. Rice-Evans,et al.  Antioxidant activity applying an improved ABTS radical cation decolorization assay. , 1999, Free radical biology & medicine.

[24]  C. Scaccini,et al.  Benzoic and cinnamic acid derivatives as antioxidants: structure-activity relation. , 1999, Journal of agricultural and food chemistry.

[25]  W. Kalt,et al.  Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity, and variety of Vaccinium species , 1998 .

[26]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[27]  H. E. Miller A simplified method for the evaluation of antioxidants , 1971 .

[28]  G. Marco A rapid method for evaluation of antioxidants , 1968 .

[29]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.