Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)

[1]  E. Kiseleva,et al.  Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells , 2007, Nature Protocols.

[2]  E. Kiseleva,et al.  A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) , 2007, Nature Protocols.

[3]  R. C. Chan,et al.  In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts. , 2006, Methods in molecular biology.

[4]  R. Heald,et al.  Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts. , 2006, Methods in molecular biology.

[5]  J. Walter,et al.  Chromosomal DNA replication in a soluble cell-free system derived from Xenopus eggs. , 2006, Methods in molecular biology.

[6]  M. Hetzer,et al.  The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly , 2003, Cell.

[7]  K. Wilson,et al.  Interference with the cytoplasmic tail of gp210 disrupts “close apposition” of nuclear membranes and blocks nuclear pore dilation , 2002, The Journal of cell biology.

[8]  M. Lohka.  Analysis of nuclear envelope assembly using extracts of Xenopus eggs. , 1998, Methods in cell biology.

[9]  K. Wilson,et al.  Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport-dependent 'envelope smoothing' event. , 1997, Journal of cell science.

[10]  K. Wilson,et al.  Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly. , 1997, Journal of cell science.

[11]  D. Forbes,et al.  Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA , 1996, The Journal of cell biology.

[12]  C. Smythe,et al.  Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts. , 1991, Methods in cell biology.

[13]  J. Maller,et al.  Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts , 1985, The Journal of cell biology.

[14]  M. Lohka,et al.  Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. , 1983, Science.