Two Methyl Jasmonate-Insensitive Mutants Show Altered Expression of AtVsp in Response to Methyl Jasmonate and Wounding

Jasmonates are plant signal molecules that are derived from lipids through the action of lipoxygenase. Jasmonates regulate gene expression during plant development and in response to water deficit, wounding, and pathogen elicitors. The signal transduction chain that mediates jasmonate action was investigated by isolating and studying two methyl jasmonate (MeJA)-insensitive mutants of Arabidopsis thaliana. The recessive mutants, jin1 and jin4, are nonallelic and neither corresponds to coi1, a previously identified MeJA-insensitive mutant. Both mutants showed reduced sensitivity to MeJA-mediated root growth inhibition as well as reduced MeJA induction of AtVsp in leaves. Expression of AtVsp in flowers was not altered in the mutants. Furthermore, MeJA modulation of the jasmonate-responsive lipoxygenase and phenylalanine ammonia lyase genes was not altered in the mutants. jin4 plants exhibited increased sensitivity to abscisic acid in seed germination assays, whereas jin1 plants showed wild-type sensitivity. Neither mutant showed altered sensitivity to ethylene in hypocotyl growth inhibition assays. jin1 and jin4 identify genes that modulate the response of AtVsp to MeJA in leaves of A. thaliana.

[1]  A. Jagendorf,et al.  Signals involved in wound-induced proteinase inhibitor II gene expression in tomato and potato plants. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[2]  B. Feys,et al.  Arabidopsis Mutants Selected for Resistance to the Phytotoxin Coronatine Are Male Sterile, Insensitive to Methyl Jasmonate, and Resistant to a Bacterial Pathogen. , 1994, The Plant cell.

[3]  S. Reinbothe,et al.  Methyl jasmonate-regulated translation of nuclear-encoded chloroplast proteins in barley (Hordeum vulgare L. cv. salome). , 1993, The Journal of biological chemistry.

[4]  J E Mullet,et al.  Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[5]  E. Farmer,et al.  Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. , 1992, The Plant cell.

[6]  S. Reinbothe,et al.  JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens. , 1994, The Plant cell.

[7]  J. Mullet,et al.  Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. , 1990, The Plant cell.

[8]  D. Hildebrand,et al.  The soybean 94-kilodalton vegetative storage protein is a lipoxygenase that is localized in paraveinal mesophyll cell vacuoles. , 1991, The Plant cell.

[9]  T. Yokota,et al.  Identification of Jasmonic Acid in Three Species of Higher Plants and Its Biological Activities , 1981 .

[10]  R. Creelman,et al.  Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Zenk,et al.  Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[12]  G. An,et al.  Identification of G-Box Sequence as an Essential Element for Methyl Jasmonate Response of Potato Proteinase Inhibitor II Promoter. , 1992, Plant physiology.

[13]  B. Vick,et al.  Biosynthesis of jasmonic Acid by several plant species. , 1984, Plant physiology.

[14]  V. Wittenbach.  Effect of pod removal on leaf senescence in soybeans. , 1982, Plant physiology.

[15]  J. D. Goeschl,et al.  Concentration dependencies of some effects of ethylene on etiolated pea, peanut, bean, and cotton seedlings. , 1975, Plant physiology.

[16]  J. Mullet,et al.  Characterization of an Arabidopsis Lipoxygenase Gene Responsive to Methyl Jasmonate and Wounding , 1993, Plant physiology.

[17]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[18]  J. Mullet,et al.  Identification of a methyl jasmonate-responsive domain in the soybean vspB promoter. , 1993, The Plant cell.

[19]  M. Becker‐André,et al.  Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene. , 1989, The EMBO journal.

[20]  R. Weidhase,et al.  Species and Tissue Specificity of Jasmonate-induced Abundant Proteins , 1989 .

[21]  M. Saniewski,et al.  Stimulation of ethylene production and ethylene-forming enzyme activity in fruits of the non-ripening nor and rin tomato mutants by methyl jasmonate , 1992 .

[22]  D. Xie,et al.  COI1-Dependent Expression of an Arabidopsis Vegetative Storage Protein in Flowers and Siliques and in Response to Coronatine or Methyl Jasmonate , 1995, Plant physiology.

[23]  Y. Koda.  The role of jasmonic acid and related compounds in the regulation of plant development. , 1992, International review of cytology.

[24]  E. Farmer,et al.  Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic Acid. , 1992, Plant physiology.

[25]  R. Weidhase,et al.  Degradation of ribulose‐1,5‐bisphosphate carboxylase and chlorophyll in senescing barley leaf segments triggered by jasmonic acid methylester, and counteraction by cytokinin , 1987 .

[26]  P. Hasegawa,et al.  Plant Defense Genes Are Synergistically Induced by Ethylene and Methyl Jasmonate. , 1994, The Plant cell.

[27]  P. Staswick,et al.  Methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an Arabidopsis thaliana mutant. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[28]  K. Skriver,et al.  The barley 60 kDa jasmonate-induced protein (JIP60) is a novel ribosome-inactivating protein. , 1994, The Plant journal : for cell and molecular biology.

[29]  V. Wittenbach.  Effect of pod removal on leaf photosynthesis and soluble protein composition of field-grown soybeans. , 1983, Plant physiology.

[30]  M. Estelle,et al.  Molecular Genetic Approaches to Plant Hormone Biology , 1991 .

[31]  F. Ausubel,et al.  An Arabidopsis thaliana Lipoxygenase Gene Can Be Induced by Pathogens, Abscisic Acid, and Methyl Jasmonate , 1993, Plant physiology.