Anaerobic degradation of dimethyl phthalate in wastewater in a UASB reactor.

Over 99% of dimethyl phthalate (DMP) and 93% of chemical oxygen demand (COD) were effectively removed in a continuous upflow anaerobic sludge blanket (UASB) reactor from a wastewater containing 600 mg/L DMP at 8h of hydraulic retention time (HRT), corresponding to a loading rate of 3g-COD/(Ld). Each gram of sludge, expressed as volatile suspended solids (VSS), had a maximum specific methanogenic activity (SMA) of 24 mg-CH(4)/(g-VSSd) using DMP as the sole carbon source. The sludge yield was estimated as 0.08 g-VSS/g-COD. During anaerobic degradation, DMP was de-esterified, first to mono-methyl phthalate (MMP) and then to phthalate, before being de-aromatized and subsequently converted to CH(4) and CO(2). The maximum specific degradation rates of DMP, MMP and phthalate were 415, 88 and 36 mg/(g-VSSd), respectively. Analysis based on polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) showed a gradual shift of microbial population with the increase of DMP loading.

[1]  J. Autian.  Toxicity and health threats of phthalate esters: review of the literature. , 1973, Environmental health perspectives.

[2]  Wei-Zhong Wu,et al.  Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells. , 2003, Biomedical and environmental sciences : BES.

[3]  Dennis R. Peterson,et al.  The environmental fate of phthalate esters: A literature review , 1997 .

[4]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.

[5]  J. Rintala,et al.  Occurrence and removal of organic pollutants in sewages and landfill leachates. , 2003, The Science of the total environment.

[6]  Yu-You Li,et al.  Degradation of phenol in wastewater in an upflow anaerobic sludge blanket reactor , 1996 .

[7]  Yingying Wang,et al.  Dimethyl phthalate ester degradation by two planktonic and immobilized bacterial consortia , 2004 .

[8]  Tong Zhang,et al.  Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities , 2000, Biotechnology Letters.

[9]  J. Toppari,et al.  Possible impact of phthalates on infant reproductive health. , 2006, International journal of andrology.

[10]  Tong Zhang,et al.  Denitrifying degradation of dimethyl phthalate , 2007, Applied Microbiology and Biotechnology.

[11]  Gatze Lettinga,et al.  Anaerobic biodegradability of phthalic acid isomers and related compounds , 1999, Biodegradation.

[12]  B. Svensson,et al.  Anaerobic degradation of phthalic acid esters during digestion of municipal solid waste under landfilling conditions , 1996, Biodegradation.

[13]  Owen P. Ward,et al.  Biodegradation of dimethyl phthalate with high removal rates in a packed-bed reactor , 2002 .

[14]  J. Tiedje,et al.  Anaerobic biodegradation of phthalic acid esters in sludge. , 1984, Environmental science & technology.

[15]  Thomas Otto,et al.  Occurrence of phthalates and bisphenol A and F in the environment. , 2002, Water research.

[16]  Tong Zhang,et al.  Aerobic degradation of diethyl phthalate by Sphingomonas sp. , 2007, Bioresource technology.