The Effect of Neuroactive Compounds on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae

ABSTRACT We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), γ-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of 42.7 ± 2.7%, followed by GABA (35.4 ± 2.0%), 5-HT (29.1 ± 2.2%), L-DOPA (19.2 ± 2.1%), epinephrine (15.2 ± 0.9%), and norepinephrine (11.0 ± 1.2%). The chemicals γ-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of 1.97 ± 1.42 and 2.37 ± 1.86 ind/cm 2 , reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery. Keywords : Neuroactive compounds, Induce settlement, Settlement rate, Larvae, Crassosterea gigas

[1]  D. Kimmel,et al.  Chemical induction of settlement behavior in larvae of the eastern oyster Crassostrea virginica (Gmelin) , 2013 .

[2]  Xiao Liang,et al.  Effects of neuroactive compounds, ions and organic solvents on larval metamorphosis of the mussel Mytilus coruscus , 2013 .

[3]  Verónica Maneiro,et al.  Effects of chemical cues on larval settlement of the flat oyster (Ostrea edulis L.): A hatchery approach , 2013 .

[4]  P. Southgate,et al.  Effects of settlement cues on behaviour and substrate attachment of hatchery reared winged pearl oyster (Pteria penguin) larvae , 2012 .

[5]  I. Martínez‐Pita,et al.  Induction of Settlement in Larvae of the Mussel Mytilus Galloprovincialis Using Neuroactive Compounds , 2012 .

[6]  Y. Hur,et al.  The Effect of Water Temperature and Salinity on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae , 2012 .

[7]  S. H. Tan,et al.  Epinephrine and l-DOPA promote larval settlement and metamorphosis of the tropical oyster, Crassostrea iredalei (Faustino, 1932): An oyster hatchery perspective , 2012 .

[8]  A. Alfaro,et al.  Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms , 2011 .

[9]  Jin-Long Yang,et al.  Larval metamorphosis of the mussel Mytilus galloprovincialis Lamarck, 1819 in response to neurotransmitter blockers and tetraethylammonium , 2011, Biofouling.

[10]  A. Alfaro,et al.  The role of bacterial biofilms and exudates on the settlement of mussel (Perna canaliculus) larvae , 2010 .

[11]  T. Blackburn,et al.  Serotonin (5-Hydroxytryptamine; 5-HT): Receptors , 2009 .

[12]  Jin-Long Yang,et al.  Induction of metamorphosis of pediveliger larvae of the mussel Mytilus galloprovincialis Lamarck, 1819 using neuroactive compounds, KCl, NH4Cl and organic solvents , 2008, Biofouling.

[13]  Yan Yan,et al.  The effect of chemical cues on settlement of pearl oyster Pinctada fucata martensii (Dunker) larvae , 2008 .

[14]  M. Najiah,et al.  Bacteria flora and heavy metals in cultivated oysters Crassostrea iredalei of Setiu Wetland, East Coast Peninsular Malaysia , 2008, Veterinary Research Communications.

[15]  Y. Hur,et al.  Larvae Growth and Biochemical Composition Change of the Pacific Oyster Crassostra gigas, Larvae during Artificial Seed Production , 2008 .

[16]  T. Subramoniam,et al.  Larval Settlement and Surfaces: Implications in Development of Antifouling strategies , 2008 .

[17]  Yaqing Chang,et al.  Enhancement of larval settlement and metamorphosis through biological and chemical cues in the abalone Haliotis diversicolor supertexta , 2006 .

[18]  Liu Wen-biao.  Induction of larval settlement and metamorphosis of Coelomactra antiquata using some chemicals , 2006 .

[19]  M. Abad,et al.  Effects of GABA and epinephrine on the settlement and metamorphosis of the larvae of four species of bivalve molluscs , 2005 .

[20]  Antonio Terlizzi,et al.  The interplay of substrate nature and biofilm formation in regulating Balanus amphitrite Darwin, 1854 larval settlement , 2004 .

[21]  K. Kang,et al.  Induction of larval settlement and metamorphosis of the abalone, Haliotis discus hannai larvae using bromomethane and potassium chloride , 2004 .

[22]  P. Qian.  Larval settlement of polychaetes , 1999, Hydrobiologia.

[23]  P. Qian,et al.  Larval settlement of the silver- or goldlip pearl oyster Pinctada maxima (Jameson) in response to natural biofilms and chemical cues , 2003 .

[24]  P. Southgate,et al.  The effect of chemical cues on settlement behaviour of blacklip pearl oyster (Pinctada margaritifera) larvae , 2002 .

[25]  P. Qian,et al.  Larval settlement and metamorphosis in the slipper limpet Crepidula onyx (Sowerby) in response to conspecific cues and the cues from biofilm , 2002 .

[26]  N. Targett,et al.  Isolation and characterization of the metamorphic inducer of the common mud crab, Panopeus herbstii. , 2001, Journal of experimental marine biology and ecology.

[27]  B. Baker,et al.  Marine Chemical Ecology , 2001 .

[28]  E. Leise,et al.  Serotonin and Nitric Oxide Regulate Metamorphosis in the Marine Snail Ilyanassa obsoleta1 , 2001 .

[29]  V. Paul,et al.  Natural Chemical Cues for Settlement and Metamorphosis of Marine-Invertebrate Larvae , 2001 .

[30]  Lin Bi.  Induction of larval settlement and metamorphosis of two oysters Crassostrea gigas and Ostrea cucullata by some chemicals , 2001 .

[31]  M. Keough,et al.  Responses of settling invertebrate larvae to bioorganic films : effects of large-scale variation in films , 1996 .

[32]  Shimizu,et al.  Visualization of cement exocytosis in the cypris cement gland of the barnacle Megabalanus rosa , 1996, The Journal of experimental biology.

[33]  E. Leise,et al.  Serotonin Injections Induce Metamorphosis in Larvae of the Gastropod Mollusc Ilyanassa obsoleta. , 1996, The Biological bulletin.

[34]  J. Widdows,et al.  Induction of metamorphosis in larvae of the oyster Crassostrea gigas using neuroactive compounds , 1995 .

[35]  M. Tamburri,et al.  Chemical identity and ecological implications of a waterborne, larval settlement cue , 1994 .

[36]  Y. Miyanohana.  The Present States and Prospects of Acoustical Survey Methods of Fisheries Resources. Contribution of the Swimbladder to the Backscattering by Fish. , 1994 .

[37]  Minoru Suzuki,et al.  Dibromomethane, a Chemical Inducer of Larval Settlement and Metamorphosis of the Sea Urchin Strongylocentroutus nudus , 1994 .

[38]  T. Leitz,et al.  The marine bacterium Alteromonas espejiana induces metamorphosis of the hydroid Hydractinia echinata , 1993 .

[39]  N. Inestrosa,et al.  Settlement of benthic marine invertebrates , 1993 .

[40]  M. Tamburri,et al.  Natural Sources and Properties of Chemical Inducers Mediating Settlement of Oyster Larvae: A Re-examination. , 1992, The Biological bulletin.

[41]  N. P. Wilkins.  Remote setting and nursery culture for shellfish growers: T.Y. Nosho and K.K. Chew (organisers). Washington Sea Grant Program, University of Washington, Seattle, WA, 68 pp., US$9.95, ISBN 0-934539-15-4 , 1992 .

[42]  J. Pawlik.  Chemical ecology of the settlement of benthic marine invertebrates , 1992 .

[43]  C. Johnson,et al.  Characteristic bacteria associated with surfaces of coralline algae: a hypothesis for bacterial induction of marine invertebrate larvae , 1991 .

[44]  W. Fitt,et al.  Ammonia Induces Settlement Behavior in Oyster Larvae. , 1990, The Biological bulletin.

[45]  W. Fitt,et al.  Competence and delay of metamorphosis in the Pacific oysterCrassostrea gigas , 1990 .

[46]  D. Morse,et al.  Artificial induction of larval metamorphosis by free fatty acids , 1990 .

[47]  D. Rittschof,et al.  Factors Controlling Attachment of Bryozoan Larvae: A Comparison of Bacterial Films and Unfilmed Surfaces , 1989 .

[48]  R. Weiner,et al.  Chemical production of cultchless oyster spat using epinephrine and norepinephrine , 1986 .

[49]  R. Weiner,et al.  Induction of settlement and metamorphosis of the pacific oyster, Crassostrea gigas (Thunberg), by L-DOPA and catecholamines , 1985 .

[50]  C. Todd.  Settlement-timing hypothesis: reply to Grant and Williamson , 1985 .

[51]  D. Morse,et al.  IONIC CONTROL OF SETTLEMENT AND METAMORPHOSIS IN LARVAL HALIOTIS RUFESCENS (GASTROPODA) , 1984 .

[52]  D. Crisp.  Overview of Research on Marine Invertebrate Larvae, 1940–1980 , 1984 .

[53]  B. Henderson.  Handling and remote setting techniques for Pacific oyster larvae Crassostrea gigas , 1983 .

[54]  D. Morse,et al.  γ-Aminobutyric Acid, a Neurotransmitter, Induces Planktonic Abalone Larvae to Settle and Begin Metamorphosis , 1979, Science.

[55]  N. Osborne.  Occurrence of GABA and taurine in the nervous systems of the dogfish and some invertebrates. , 1971, Comparative and general pharmacology.