In vitro growth of human adult bone-derived cells on hydroxyapatite plasma-sprayed coatings.

The growth of adult human bone-derived cells on hydroxyapatite (HA) plasma-sprayed coatings was investigated. Such cells were difficult to grow on original plasma-sprayed coatings, even following rinsing and rubbing down. We obtained cell growth only on samples previously immersed 15 or 22 days in complete culture medium. We describe a dissolution/precipitation phenomenon on the HA coating surface assessed by modifications of Ca and P concentrations in the culture medium, by the transformation of the HA coating into carbonated HA (X-ray diffraction and infrared spectrometry and by the presence demonstrated by scanning electron microscopy of spherocrystallites on the HA after 15 days of immersion. Our results show that adult human bone-derived cells are apparently particularly sensitive to the changes in the coating surface induced by liquid immersion. We raise the question of the limits of in vitro investigations on bioactive ceramics such as HA plasma-sprayed coatings susceptible to modification by simple immersion in aqueous solutions such as cell culture medium or physiologic saline.

[1]  B. Labat,et al.  Effects of gamma-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts. , 1995, Journal of biomedical materials research.

[2]  C. G. Groot,et al.  Preliminary ageing study of bioactive glass in a cell culture model , 1995 .

[3]  J. Ong,et al.  Cellular response to well-characterized calcium phosphate coatings and titanium surfaces in vitro. , 1995, Journal of biomedical materials research.

[4]  S. Radin,et al.  Effect of bioactive ceramic composition and structure on in vitro behavior. III. Porous versus dense ceramics. , 1994, Journal of biomedical materials research.

[5]  R. Bareille,et al.  Evaluation of cell colonization on biomaterials: preventing cell attachment to plastic containers. , 1994, Biomaterials.

[6]  A. Dekker,et al.  Cytotoxicity investigations of plasma sprayed calcium phosphate coatings , 1994 .

[7]  C. Ju,et al.  Morphologic variation in plasma-sprayed hydroxyapatite-bioactive glass composite coatings in Hank's solution. , 1994, Journal of biomedical materials research.

[8]  J. Jansen,et al.  A histological evaluation of the effect of hydroxyapatite coating on interfacial response , 1993 .

[9]  U. Joos,et al.  Mechanisms and structure of the bond between bone and hydroxyapatite ceramics. , 1993, Journal of biomedical materials research.

[10]  C. V. van Blitterswijk,et al.  The ultrastructure of the bone-hydroxyapatite interface in vitro. , 1992, Journal of biomedical materials research.

[11]  T. Yamamuro,et al.  Surface reactions of calcium phosphate ceramics to various solutions. , 1990, Journal of biomedical materials research.

[12]  K. Hayashi,et al.  Evaluation of metal implants coated with several types of ceramics as biomaterials. , 1989, Journal of biomedical materials research.

[13]  T. Yamamuro,et al.  The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media. , 1989, Journal of biomedical materials research.

[14]  C. Guerriero,et al.  Adult human bone cells from jaw bones cultured on plasma-sprayed or polished surfaces of titanium or hydroxylapatite discs , 1996 .

[15]  S. Downes,et al.  Comparison of the early production of extracellular matrix on dense hydroxyapatite and hydroxyapatite-coated titanium in cell and organ culture. , 1995, Biomaterials.

[16]  S. Ban,et al.  Calcium phosphate precipitation on the surface of HA-G-Ti composite under physiologic conditions. , 1994, Journal of biomedical materials research.

[17]  P. Frayssinet,et al.  Comparative biological properties of HA plasma-sprayed coatings having different crystallinities , 1994 .

[18]  M. Dunn,et al.  In vitro evaluation of amorphous calcium phosphate and poorly crystallized hydroxyapatite coatings on titanium implants. , 1993, Journal of biomedical materials research.

[19]  P. Patka,et al.  Plasma-sprayed coatings of tetracalciumphosphate, hydroxyl-apatite, and alpha-TCP on titanium alloy: an interface study. , 1991, Journal of biomedical materials research.