Visual quantification of Hg on a microfluidic paper-based analytical device using distance-based detection technique

We presented a distance-based detection method for visual quantification of mercury ions on a microfluidic paper-based analytical device (μPAD). Dithizone in NaOH solution was used as chromogenic reagent and deposited onto paper channel delimited by hydrophobic wax barrier. Reactions happened between mercury ions and dithizone to form an insoluble colored complex, producing colored precipitate on the paper channel. The length of colored precipitate could be readily measured using the printed ruler along each device. The length of precipitate increase linearly with the mercury concentrations, mercury in sample solution could be quantified by measuring the length of the colored precipitate. Being free of any electronic instruments, this method has the advantages of portability, ease of use, low cost and disposability. This presented method was used to detect mercury ions in a synthetic sample, demonstrating its potential in on-site and real time analysis.We presented a distance-based detection method for visual quantification of mercury ions on a microfluidic paper-based analytical device (μPAD). Dithizone in NaOH solution was used as chromogenic reagent and deposited onto paper channel delimited by hydrophobic wax barrier. Reactions happened between mercury ions and dithizone to form an insoluble colored complex, producing colored precipitate on the paper channel. The length of colored precipitate could be readily measured using the printed ruler along each device. The length of precipitate increase linearly with the mercury concentrations, mercury in sample solution could be quantified by measuring the length of the colored precipitate. Being free of any electronic instruments, this method has the advantages of portability, ease of use, low cost and disposability. This presented method was used to detect mercury ions in a synthetic sample, demonstrating its potential in on-site and real time analysis.

[1]  N. Pourreza,et al.  Highly selective and portable chemosensor for mercury determination in water samples using curcumin nanoparticles in a paper based analytical device , 2016 .

[2]  Orawon Chailapakul,et al.  Simple and rapid colorimetric detection of Hg(II) by a paper-based device using silver nanoplates. , 2012, Talanta.

[3]  Min Zhang,et al.  Terbium(III)/gold nanocluster conjugates: the development of a novel ratiometric fluorescent probe for mercury(II) and a paper-based visual sensor. , 2015, The Analyst.

[4]  Meng Zhang,et al.  Three-dimensional paper-based electrochemiluminescence device for simultaneous detection of Pb2+ and Hg2+ based on potential-control technique. , 2013, Biosensors & bioelectronics.

[5]  Zhi Zhu,et al.  Microfluidic Distance Readout Sweet Hydrogel Integrated Paper-Based Analytical Device (μDiSH-PAD) for Visual Quantitative Point-of-Care Testing. , 2016, Analytical chemistry.

[6]  C. Henry,et al.  Multiplexed paper analytical device for quantification of metals using distance-based detection. , 2015, Lab on a chip.

[7]  Longfei Cai,et al.  Defining microchannels and valves on a hydrophobic paper by low-cost inkjet printing of aqueous or weak organic solutions. , 2015, Biomicrofluidics.

[8]  D. M. Brum,et al.  Development of a method for total Hg determination in oil samples by cold vapor atomic absorption spectrometry after its extraction induced by emulsion breaking. , 2015, Talanta.

[9]  Arben Merkoçi,et al.  Rapid and highly sensitive detection of mercury ions using a fluorescence-based paper test strip with an N-alkylaminopyrazole ligand as a receptor , 2012 .

[10]  S. T. Phillips,et al.  Fluidic timers for time-dependent, point-of-care assays on paper. , 2010, Analytical chemistry.

[11]  Jilie Kong,et al.  A Simple Paper-Based Colorimetric Device for Rapid Mercury(II) Assay , 2016, Scientific Reports.

[12]  Charles S Henry,et al.  Simple, distance-based measurement for paper analytical devices. , 2013, Lab on a chip.

[13]  Orawon Chailapakul,et al.  Microfluidic paper-based analytical device for aerosol oxidative activity. , 2013, Environmental science & technology.

[14]  Charles S Henry,et al.  Development of a paper-based analytical device for colorimetric detection of select foodborne pathogens. , 2012, Analytical chemistry.

[15]  Mohsen Kompany-Zareh,et al.  Application of generalized artificial neural networks coupled with an orthogonal design to optimization of a system for the kinetic spectrophotometric determination of Hg(II) , 2002 .

[16]  P. Perämäki,et al.  Internal standardization using a dual mode sample introduction system in the determination of As by HG-ICP-MS , 2016 .

[17]  C. Zheng,et al.  Chemical vapor generation from an ionic liquid using a solid reductant: determination of Hg, As and Sb by atomic fluorescence spectrometry , 2016 .

[18]  Hu Wang,et al.  Paper-based three-dimensional microfluidic device for monitoring of heavy metals with a camera cell phone , 2014, Analytical and Bioanalytical Chemistry.

[19]  Sang Mi Park,et al.  Sensitized spectrophotometric determination of trace Hg(II) in benzalkonium chloride media , 2002 .

[20]  Lin Lu,et al.  Determination of Apparent Amylose Content in Rice by Using Paper-Based Microfluidic Chips. , 2015, Journal of agricultural and food chemistry.

[21]  G. Whitesides,et al.  Patterned paper as a platform for inexpensive, low-volume, portable bioassays. , 2007, Angewandte Chemie.