Logic Gates Based on Circular DNA Strand Displacement and a Fluorescent Agent

We constructed a three-input OR logic gate based on a single-strand DNA circle and a two-input AND logic gate measuring fluorescence signals produced by strand displacement to detect the outputs. The simple, cost effective OR and AND logic gates produced the expected results, demonstrating their feasibility for future use in DNA computing. Fluorescently labeled DNA oligonucleotide inputs were initially hybridized with a quencher strand, which was displaced when the input strands hybridized to circular DNA.

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