Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA‐1 subunit‐specific monoclonal antibodies

We have attempted to characterize the rat leukocyte integrin, CD11/CD18, by the use of newly generated monoclonal antibodies (mAb) WT.1 (anti‐CD11a) and WT.3 (anti‐CD18) in conjunction with an mAb, OX42, reactive with a rat integrin‐like molecule, with respect to the biochemistry, cellular distribution and function. The conclusion that the mAb WT.1 and WT.3 specifically recognize the rat CD11a and CD18, respectively, was based on: (a) their ability to inhibit homotypic aggregation of splenic concanavalin A (Con A) blasts; (b) sodium dodecyl sulfate‐polyacrylamide gel electrophoretic analysis of the antigens recognized; (c) their ability to inhibit binding of Con A blasts to the purified ligand, namely the ICAM‐1 antigen and (d) their blocking abilities in mixed leukocyte reaction.

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