Detoxification of Jatropha curcas seed cake using chemical treatment: Analysis with a central composite rotatable design

Abstract Jatropha curcas is a plant whose seeds are rich in oil that can be used for biofuel production, and the seed cake has a high nutritional value. The presence of phorbol ester (PE), a toxic constituent, restricts the cake use in animal food. The detoxification of this by-product is the aim of this work and the seed cake was extracted using Soxhlet for PE removal. A statistical analysis was used to evaluate the PE extraction as function of extraction time, mixture of methanol and ethanol and solute/solvent ratio. The detoxification was reached, reducing the PE content in 97.30% (0.10 mg/g) using a mixture of 50% of methanol, extraction time of 8 h and solute/solvent ratio of 1:10 (w/v). The treatments had a positive effect on protein content and the dry matter, ash and dietary fiber content ranged from 88.47 to 90.70%, 4.89–6.16% and 33.09–38.23%, respectively.

[1]  K. Becker,et al.  Toxicity of Jatropha curcas phorbol esters in mice. , 2010, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[2]  José Maria Rodrigues da Luz,et al.  Bio-Detoxification of Jatropha Seed Cake and Its Use in Animal Feed , 2012 .

[3]  Harinder P. S. Makkar,et al.  Detoxification of a toxic variety of Jatropha curcas using heat and chemical treatments, and preliminary nutritional evaluation with rats , 2003 .

[4]  R. Roscoe,et al.  Caracterização química de sementes e tortas de pinhão‑manso, nabo‑forrageiro e crambe , 2009 .

[5]  Harinder P. S. Makkar,et al.  Chemical composition and effect of heat on organic matter- and nitrogen-degradability and some antinutritional components of Jatropha meal , 1997 .

[6]  Michael Wink,et al.  Studies on nutritive potential and toxic constituents of different provenances of Jatropha curcas , 1997 .

[7]  K. Becker,et al.  Nutritional studies on rats and fish (carp Cyprinus carpio) fed diets containing unheated and heated Jatropha curcas meal of a non-toxic provenance , 1999, Plant foods for human nutrition.

[8]  K. Becker,et al.  Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico , 2006 .

[9]  Harinder P. S. Makkar,et al.  Comparative evaluation of non-toxic and toxic varieties of Jatropha curcas for chemical composition, digestibility, protein degradability and toxic factors , 1998 .

[10]  S E Adam,et al.  Effects of Jatropha curcas on Calves , 1979, Veterinary pathology.

[11]  E. Hecker,et al.  Irritant phorbol derivatives from four Jatropha species , 1984 .

[12]  K. Becker,et al.  Dietary inclusion of detoxified Jatropha curcas kernel meal: effects on growth performance and metabolic efficiency in common carp, Cyprinus carpio L. , 2010, Fish Physiology and Biochemistry.

[13]  K. Narasimhamurthy,et al.  Toxicity studies of detoxified Jatropha meal (Jatropha curcas) in rats. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[14]  A. Ratnadass,et al.  Potential of sorghum and physic nut (Jatropha curcas) for management of plant bugs (Hemiptera: Miridae) and cotton bollworm (Helicoverpa armigera) on cotton in an assisted trap-cropping strategy , 2007 .

[15]  R. Singh,et al.  Jatropha Poisoning in Children. , 2010, Medical journal, Armed Forces India.

[16]  C. Parsons,et al.  Nutritional evaluation of soybean meals varying in oligosaccharide content. , 2000, Poultry science.

[17]  Hui Zhang,et al.  Evaluation of detoxification methods on toxic and antinutritional composition and nutritional quality of proteins in Jatropha curcas meal. , 2011, Journal of agricultural and food chemistry.

[18]  M. J. Mulky,et al.  Toxicological studies on ratanjyot oil. , 1995, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[19]  K. Becker,et al.  Isolation, stability and bioactivity of Jatropha curcas phorbol esters. , 2012, Fitoterapia.

[20]  K. Becker,et al.  Phorbol Esters: Structure, Biological Activity, and Toxicity in Animals , 2007, International journal of toxicology.

[21]  M. Pleasant,et al.  Effects Of Dietary Jatropha curcas Meal on Percent Packed Cell Volume, Serum Glucose, Cholesterol and Triglyceride Concentration and Alpha-Amylase Activity of Weaned Fattening Pigs. , 2006 .

[22]  P. Srinophakun,et al.  Prospect of Deoiled Jatropha curcas Seedcake as Fertilizer for Vegetables Crops – A Case Study , 2011 .

[23]  Ashwani Kumar,et al.  An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): A review , 2008 .

[24]  Prasanna Kumar Panda,et al.  Effects of Fertilizer Application on Growth and Yield of Jatropha curcas L. in an Aeric Tropaquept of Eastern India , 2011 .

[25]  Martin Mittelbach,et al.  Novel 12-deoxy-16-hydroxyphorbol diesters isolated from the seed oil of Jatropha curcas. , 2002, Journal of natural products.

[26]  Harinder P. S. Makkar,et al.  Jatropha curcas, a promising crop for the generation of biodiesel and value-added coproducts , 2009 .

[27]  Hui Zhang,et al.  Comparative evaluation of Jatropha curcas L. seed meals obtained by different methods of defatting on toxic, antinutritional and nutritive factors , 2014, Journal of Food Science and Technology.