Interchromatidal central ridge and transversal symmetry in early metaphasic human chromosome one

The topographic structure of Giemsa‐banded (G‐banded) early metaphase human chromosomes adsorbed on glass was analyzed by atomic force microscope using amplitude modulation mode (AM‐AFM). Longitudinal height measurements for early metaphasic human chromosomes showed a central ridge that was further characterized by transversal height measurements. The heterochromatic regions displayed a high level of transversal symmetry, while the euchromatic ones presented several peaks across the transversal height measurements. We suggest that this central ridge and symmetry patterns point out a transitional arrangement of the early metaphase chromosome and support evidence for interchromatidal interactions prior to disjunction. Copyright © 2008 John Wiley & Sons, Ltd.

[1]  Yong Chen,et al.  Chromosome imaging by atomic force microscopy: influencing factors and comparative evaluation , 2006, Journal of Genetics.

[2]  Yong Chen,et al.  Atomic force microscopic examination of chromosomes treated with trypsin or ethidium bromide. , 2006, Chemical & pharmaceutical bulletin.

[3]  T. Ushiki,et al.  Atomic force microscopy of native human metaphase chromosomes in a liquid. , 2006, Archives of histology and cytology.

[4]  T. Ushiki,et al.  The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy. , 2005, Archives of histology and cytology.

[5]  M. Ergun,et al.  Chromosomes of a balanced translocation case evaluated with atomic force microscopy , 2005 .

[6]  Yuekan Jiao,et al.  Accurate height and volume measurements on soft samples with the atomic force microscope. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[7]  T. Ushiki,et al.  Atomic force microscopy of human metaphase chromosomes after differential staining of sister chromatids. , 2004, Archives of histology and cytology.

[8]  Tai Wang,et al.  Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion. , 2004, Journal of experimental botany.

[9]  S. Grigoryev,et al.  Keeping fingers crossed: heterochromatin spreading through interdigitation of nucleosome arrays , 2004, FEBS letters.

[10]  A. T. Sumner.  Scanning electron microscopy of mammalian chromosomes from prophase to telophase , 1991, Chromosoma.

[11]  Rachel A. Horowitz-Scherer,et al.  Chromatin Compaction by Human MeCP2 , 2003, Journal of Biological Chemistry.

[12]  K. Nasmyth,et al.  Chromosomal Cohesin Forms a Ring , 2003, Cell.

[13]  Rachel A. Horowitz-Scherer,et al.  Chromatin Compaction by Human MeCP2 ASSEMBLY OF NOVEL SECONDARY CHROMATIN STRUCTURES IN THE ABSENCE OF DNA METHYLATION* , 2003 .

[14]  T. Ushiki,et al.  The structure of human metaphase chromosomes: its histological perspective and new horizons by atomic force microscopy. , 2002, Archives of histology and cytology.

[15]  Ricardo Garcia,et al.  Dynamic atomic force microscopy methods , 2002 .

[16]  T. Ushiki,et al.  Three-dimensional structure of G-banded human metaphase chromosomes observed by atomic force microscopy. , 2001, Archives of histology and cytology.

[17]  R. Stark,et al.  GTG banding pattern on human metaphase chromosomes revealed by high resolution atomic‐force microscopy , 2001, Journal of microscopy.

[18]  M. Miles,et al.  Human chromosome structure studied by scanning force microscopy after an enzymatic digestion of the covering cell material. , 2000, Ultramicroscopy.

[19]  M. Miles,et al.  Selective cleaning of the cell debris in human chromosome preparations studied by scanning force microscopy. , 1999, Journal of structural biology.

[20]  William S. Klug,et al.  Concepts of Genetics , 1999 .

[21]  S. Kudo.  Methyl-CpG-Binding Protein MeCP2 Represses Sp1-Activated Transcription of the Human Leukosialin Gene When the Promoter Is Methylated , 1998, Molecular and Cellular Biology.

[22]  Stefan Thalhammer,et al.  The AFM as a tool for chromosomal dissection – the influence of physical parameters , 1998 .

[23]  W. Fritzsche,et al.  Application of atomic force microscopy to visualization of DNA, chromatin, and chromosomes. , 1997, Critical reviews in eukaryotic gene expression.

[24]  J. Wienberg,et al.  Analysis of banded human chromosomes and in situ hybridization patterns by scanning force microscopy. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[25]  K. Adolph,et al.  Assembly of chromatin fibers into metaphase chromosomes analyzed by transmission electron microscopy and scanning electron microscopy. , 1986, Biophysical journal.

[26]  C. Harrison,et al.  High-resolution scanning electron microscopy of human metaphase chromosomes. , 1982, Journal of cell science.

[27]  D. W. Bath.  Surface ultrastructure of trypsin-banded chromosomes. , 1976, Experimental cell research.