论文信息 - Optimized CUBIC protocol for 3 D imaging of chicken embryos at single-cell resolution

Optimized CUBIC protocol for 3 D imaging of chicken embryos at single-cell resolution

The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole earlystage chicken embryos, and in the whole brains of late-stage embryos. D ev el o pm en t • A dv an ce a rt ic le

[1] K. E. Godden,et al. Early Expression of Hypocretin/Orexin in the Chick Embryo Brain , 2014, PloS one.

[2] K. E. Godden,et al. Activation of state-regulating neurochemical systems in newborn and embryonic chicks , 2016, Neuroscience.

[3] Johannes E. Schindelin,et al. Fiji: an open-source platform for biological-image analysis , 2012, Nature Methods.

[4] Henrik Mouritsen,et al. Cryptochromes and neuronal-activity markers colocalize in the retina of migratory birds during magnetic orientation. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[5] Dimitri Perrin,et al. Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging , 2015, Nature Protocols.

[6] Benjamin Schmid,et al. A high-level 3D visualization API for Java and ImageJ , 2010, BMC Bioinformatics.