Large-Scale Targeted Protein Quantification Using WiSIM-DIA on an Orbitrap Fusion Tribrid Mass Spectrometer

Introduction Proteomics studies are rapidly turning from qualitative to largely quantitative experiments in order to better understand the functions and interactions of proteins in biological systems and to verify long lists of putative biomarkers. The extreme complexity and large dynamic range of proteins in standard biological samples challenge traditional data-dependent workflows. They necessitate very fast tandem mass spectrometry (MS/MS) with simultaneous high sensitivity and reproducibility. Without these, it is difficult to achieve the depth of sample interrogation necessary to identify and quantify the same targets in replicate runs to accurately determine differences amongst samples.