Estimation of genetic variances and heritability in novel crosses

18 Present investigation was carried out to assess the heterotic potential and combining ability of immortal restorer lines [consisting of two recombinant inbred lines (RILs) and two doubled 20 haploid lines (DHLs)] developed from an elite rice hybrid, KRH-2 by crossing them with 21 three popular WA-CMS lines, IR58025A, CRMS32A and APMS6A through line × tester 22 analysis. The doubled haploid line 1 (DHL-1) was observed to be a good general combiner 23 for total grain yield per plant (YLD) and other yield component traits and among the CMS 24 lines, IR58025A was observed to be the best combiner as it showed positive significant 25 values for the traits viz., total grain yield per plant, panicle length and spikelet fertility. Higher preponderance of the variance associated with specific combining ability (SCA) as 27 compared to general combining ability (GCA) variance was observed for most of the traits 28 indicated the predominant role of non-additive gene action in the expression of the traits. Out 29 of twelve novel crosses between the immortal restorer lines derived from KRH-2 and the 30 WA-CMS lines, 66.66% (eight crosses) showed significant and desirable SCA effects for the 31 traits viz., total grain yield per plant, days to fifty percent flowering, plant height, flag leaf 32 length, flag leaf width, number of filled grains per panicle and spikelet fertility. Two crosses 33 IR58025A/RIL-24 and CRMS32A/RIL-24 were observed to be the most promising cross 34 combinations showing standard heterosis of >50% for YLD trait (as compared with KRH-2) 35 with higher prevalence of GCA and SCA, respectively. Heterotic yield advantage of 36 IR58025A/RIL-24 and CRMS32A/RIL-24 was 77.05% and 54.74%, respectively over KRH-37 2 and these can be utilized for developing commercial hybrids. The present study also indicates the potentiality of RILs in providing useful parental lines for developing heterotic hybrids which are hard to get from outside sources in the new intellectual property regime.

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