Photodynamic Treatment of Red Blood Cell Concentrates For Virus Inactivation Enhances Red Blood Cell Aggregation: Protection with Antioxidants

Abstract— Photodynamic treatment (PDT) using phthalocyanines and red light appears to be a promising procedure for decontamination of red blood cell (RBC) concentrates for transfusion. A possible complication of this treatment may be induced aggregation of RBC. The production of RBC aggregates was measured with a novel computerized cell flow properties analyzer (CFA). The PDT of RBC concentrates with sulfonated aluminum phthalocy‐anine (AIPcS4) and the silicon phthalocyanine Pc 4 under virucidal conditions markedly enhanced RBC aggregation and higher shear stress was required to disperse these aggregates. The clusters of cells were huge and abnormally shaped, unlike the rouleaux formed by untreated RBC. This aggregation was prevented when a mixture of antioxidants was included during PDT. Addition of the antioxidants after PDT reduced aggregation only partially. It is concluded that inclusion of antioxidants during PDT of RBC concentrates prior to transfusion may reduce or eliminate the hemodynamic risk that the virucidal treatment may present to the recipient.

[1]  E. Ben-hur,et al.  Virus inactivation in blood. , 1996, AIDS.

[2]  D. Kessel,et al.  Delayed Oxidative Photodamage induced by Photodynamic Therapy , 1996, Photochemistry and photobiology.

[3]  P. Gottlieb,et al.  Inactivation by phthalocyanine photosensitization of multiple forms of human immunodeficiency virus in red cell concentrates , 1996, Transfusion.

[4]  H. Klein,et al.  Safety of the blood supply. , 1995, JAMA.

[5]  Y Mahler,et al.  Monitoring of erythrocyte aggregate morphology under flow by computerized image analysis. , 1995, Biorheology.

[6]  M. Reid,et al.  Selective protection against IgG binding to red cells treated with phthalocyanines and red light for virus inactivation , 1995, Transfusion.

[7]  N. Geacintov,et al.  Virus inactivation in red cell concentrates by photosensitization with phthalocyanines: protection of red cells but not of vesicular stomatitis virus with a water‐soluble analogue of vitamin E , 1995, Transfusion.

[8]  G. Lowe,et al.  Increased Red Cell Aggregation in Diabetes Mellitus: Association with Cardiovascular Risk Factors , 1993, Diabetic medicine : a journal of the British Diabetic Association.

[9]  N. Geacintov,et al.  IMPORTANCE OF TYPE I AND TYPE II MECHANISMS IN THE PHOTODYNAMIC INACTIVATION OF VIRUSES IN BLOOD WITH ALUMINUM PHTHALOCYANINE DERIVATIVES , 1992, Photochemistry and photobiology.

[10]  A. Libretti,et al.  Microcirculation and Hemorheology in NIDDM Patients , 1990, Angiology.

[11]  J G Jones,et al.  New Aspects of Red Cell Aggregation , 1990 .

[12]  N. Mohandas,et al.  Oxidation-induced changes in microrheologic properties of the red blood cell membrane. , 1990, Blood.

[13]  S Chien,et al.  Mechanics of Rouleau formation. , 1981, Biophysical journal.

[14]  H. H. Lipowsky,et al.  The Distribution of Blood Rheological Parameters in the Microvasculature of Cat Mesentery , 1978, Circulation research.

[15]  Yona Mahler,et al.  Monitoring of red blood cell aggregability in a flow-chamber by computerized image analysis , 1994 .

[16]  E. Evans,et al.  Rheology of blood cells , 1987 .

[17]  Shu Chien,et al.  Physiological and pathophysiological significance of hemorheology , 1987 .

[18]  S. Chien,et al.  Chapter 26 – Biophysical Behavior of Red Cells in Suspensions , 1975 .

[19]  M. H. Knisely,et al.  Sludged Blood. , 1947, Science.